Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   D9C10_RS22000 Genome accession   NZ_CP032853
Coordinates   4052314..4052763 (-) Length   149 a.a.
NCBI ID   WP_121549304.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis strain MH-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4047314..4057763
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D9C10_RS21960 (D9C10_21960) - 4047645..4047842 (+) 198 WP_121509395.1 hypothetical protein -
  D9C10_RS21965 (D9C10_21965) yckC 4047862..4048317 (+) 456 WP_014478793.1 RDD family protein -
  D9C10_RS21970 (D9C10_21970) yckD 4048399..4048731 (+) 333 WP_014478794.1 YckD family protein -
  D9C10_RS21975 (D9C10_21975) - 4048859..4049281 (+) 423 WP_014478795.1 MarR family transcriptional regulator -
  D9C10_RS21980 (D9C10_21980) - 4049269..4049589 (+) 321 WP_014478796.1 DUF3147 family protein -
  D9C10_RS21985 (D9C10_21985) - 4049608..4050352 (+) 745 Protein_4244 AAA family ATPase -
  D9C10_RS21990 (D9C10_21990) bglC 4050425..4051852 (+) 1428 Protein_4245 6-phospho-beta-glucosidase -
  D9C10_RS21995 (D9C10_21995) nin/comJ 4051889..4052287 (-) 399 WP_103330359.1 DNA-entry nuclease inhibitor Regulator
  D9C10_RS22000 (D9C10_22000) nucA/comI 4052314..4052763 (-) 450 WP_121549304.1 DNA-entry nuclease Machinery gene
  D9C10_RS22005 (D9C10_22005) tlpC 4052931..4054652 (-) 1722 Protein_4248 methyl-accepting chemotaxis protein TlpC -
  D9C10_RS22010 (D9C10_22010) hxlB 4054763..4055320 (-) 558 WP_121549305.1 6-phospho-3-hexuloisomerase -
  D9C10_RS22015 (D9C10_22015) hxlA 4055326..4055958 (-) 633 WP_103330355.1 3-hexulose-6-phosphate synthase -
  D9C10_RS22020 (D9C10_22020) hxlR 4056190..4056552 (+) 363 WP_003246265.1 transcriptional activator HxlR -
  D9C10_RS23000 - 4056559..4056795 (-) 237 WP_205589300.1 hypothetical protein -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.48 Da        Isoelectric Point: 4.5282

>NTDB_id=319287 D9C10_RS22000 WP_121549304.1 4052314..4052763(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain MH-1]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKMSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=319287 D9C10_RS22000 WP_121549304.1 4052314..4052763(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain MH-1]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAATGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTTCCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment