Detailed information
Bioinformatically predicted
Overview
| Name | nucA/comI | Type | Machinery gene |
| Locus tag | D9C09_RS17400 | Genome accession | NZ_CP032852 |
| Coordinates | 3250131..3250541 (-) | Length | 136 a.a. |
| NCBI ID | WP_009967785.1 | Uniprot ID | A0A6I4D881 |
| Organism | Bacillus subtilis subsp. subtilis strain GFR-12 | ||
| Function | cleavage of dsDNA into ssDNA (predicted from homology) DNA processing |
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Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 3245498..3273403 | 3250131..3250541 | within | 0 |
Gene organization within MGE regions
Location: 3245498..3273403
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| D9C09_RS17375 (D9C09_17375) | yqeF | 3245665..3246396 (-) | 732 | WP_003229964.1 | SGNH/GDSL hydrolase family protein | - |
| D9C09_RS17380 (D9C09_17380) | cwlH | 3246648..3247400 (-) | 753 | WP_069837642.1 | N-acetylmuramoyl-L-alanine amidase CwlH | - |
| D9C09_RS17385 (D9C09_17385) | yqeD | 3247587..3248213 (+) | 627 | WP_014480319.1 | TVP38/TMEM64 family protein | - |
| D9C09_RS17390 (D9C09_17390) | gnd | 3248232..3249125 (-) | 894 | WP_069837643.1 | phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) | - |
| D9C09_RS17395 (D9C09_17395) | yqeB | 3249376..3250098 (+) | 723 | WP_014480321.1 | hypothetical protein | - |
| D9C09_RS17400 (D9C09_17400) | nucA/comI | 3250131..3250541 (-) | 411 | WP_009967785.1 | sporulation-specific Dnase NucB | Machinery gene |
| D9C09_RS17405 (D9C09_17405) | sigK | 3250737..3251465 (+) | 729 | WP_013308023.1 | RNA polymerase sporulation sigma factor SigK | - |
| D9C09_RS17410 (D9C09_17410) | - | 3251465..3251563 (+) | 99 | WP_031600702.1 | hypothetical protein | - |
| D9C09_RS23025 | - | 3251560..3251772 (-) | 213 | Protein_3365 | recombinase family protein | - |
| D9C09_RS17420 (D9C09_17420) | fumC | 3251991..3253379 (-) | 1389 | WP_014480325.1 | class II fumarate hydratase | - |
| D9C09_RS17425 (D9C09_17425) | - | 3253546..3254436 (+) | 891 | WP_014480326.1 | LysR family transcriptional regulator | - |
| D9C09_RS17430 (D9C09_17430) | - | 3255378..3255773 (+) | 396 | WP_014480327.1 | VOC family protein | - |
| D9C09_RS17440 (D9C09_17440) | - | 3256513..3257640 (-) | 1128 | WP_014480328.1 | Rap family tetratricopeptide repeat protein | - |
| D9C09_RS23155 (D9C09_17445) | - | 3257822..3259748 (+) | 1927 | Protein_3370 | T7SS effector LXG polymorphic toxin | - |
| D9C09_RS17450 (D9C09_17450) | - | 3259762..3260049 (+) | 288 | WP_014480331.1 | hypothetical protein | - |
| D9C09_RS17460 (D9C09_17460) | - | 3260452..3260892 (+) | 441 | WP_014480332.1 | SMI1/KNR4 family protein | - |
| D9C09_RS17465 (D9C09_17465) | - | 3260991..3261443 (+) | 453 | WP_014480333.1 | SMI1/KNR4 family protein | - |
| D9C09_RS17470 (D9C09_17470) | cdiI | 3261540..3261899 (+) | 360 | WP_014480334.1 | ribonuclease toxin immunity protein CdiI | - |
| D9C09_RS17475 (D9C09_17475) | - | 3262004..3262483 (+) | 480 | WP_224588637.1 | hypothetical protein | - |
| D9C09_RS22585 | - | 3262787..3262990 (-) | 204 | WP_123772462.1 | hypothetical protein | - |
| D9C09_RS17480 (D9C09_17480) | - | 3263070..3263303 (+) | 234 | WP_224588641.1 | hypothetical protein | - |
| D9C09_RS17485 (D9C09_17485) | atxG | 3263560..3264137 (+) | 578 | Protein_3378 | suppressor of fused domain protein | - |
| D9C09_RS17490 (D9C09_17490) | - | 3264247..3264537 (+) | 291 | WP_014480337.1 | contact-dependent growth inhibition system immunity protein | - |
| D9C09_RS17500 (D9C09_17500) | istA | 3265378..3266925 (+) | 1548 | WP_014480339.1 | IS21 family transposase | - |
| D9C09_RS17505 (D9C09_17505) | istB | 3266922..3267680 (+) | 759 | WP_014479891.1 | IS21-like element helper ATPase IstB | - |
| D9C09_RS23045 | - | 3267998..3268095 (-) | 98 | Protein_3382 | N-acetylmuramoyl-L-alanine amidase | - |
| D9C09_RS17515 (D9C09_17515) | - | 3268300..3268359 (+) | 60 | WP_076458313.1 | putative holin-like toxin | - |
| D9C09_RS23255 (D9C09_17520) | - | 3268646..3269082 (-) | 437 | Protein_3384 | phage tail tube protein | - |
| D9C09_RS22720 | terS | 3269080..3269645 (-) | 566 | Protein_3385 | phage terminase small subunit | - |
| D9C09_RS22590 | - | 3269772..3270077 (+) | 306 | WP_123772463.1 | hypothetical protein | - |
| D9C09_RS23060 | - | 3270250..3270315 (-) | 66 | Protein_3387 | hypothetical protein | - |
| D9C09_RS17530 (D9C09_17530) | - | 3270470..3270949 (-) | 480 | WP_014480344.1 | hypothetical protein | - |
| D9C09_RS17535 (D9C09_17535) | - | 3271566..3271832 (+) | 267 | WP_033881358.1 | hypothetical protein | - |
| D9C09_RS17540 (D9C09_17540) | - | 3271971..3272123 (-) | 153 | WP_049832653.1 | XtrA/YqaO family protein | - |
| D9C09_RS17545 (D9C09_17545) | - | 3272206..3272328 (-) | 123 | Protein_3391 | RusA family crossover junction endodeoxyribonuclease | - |
| D9C09_RS17550 (D9C09_17550) | - | 3272291..3272539 (-) | 249 | Protein_3392 | hypothetical protein | - |
| D9C09_RS23065 | - | 3272684..3272914 (-) | 231 | WP_224588644.1 | hypothetical protein | - |
| D9C09_RS17560 (D9C09_17560) | - | 3273223..3273403 (-) | 181 | Protein_3394 | hypothetical protein | - |
Sequence
Protein
Download Length: 136 a.a. Molecular weight: 14967.97 Da Isoelectric Point: 5.1853
>NTDB_id=319200 D9C09_RS17400 WP_009967785.1 3250131..3250541(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain GFR-12]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ
Nucleotide
Download Length: 411 bp
>NTDB_id=319200 D9C09_RS17400 WP_009967785.1 3250131..3250541(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain GFR-12]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| nucA/comI | Bacillus subtilis subsp. subtilis str. 168 |
62.609 |
84.559 |
0.529 |