Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   D8Z77_RS07350 Genome accession   NZ_CP032848
Coordinates   1575849..1577213 (+) Length   454 a.a.
NCBI ID   WP_096888201.1    Uniprot ID   A0AAE6WN44
Organism   Brevibacillus laterosporus strain Bl-zj     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1570849..1582213
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D8Z77_RS07330 (D8Z77_07330) - 1570998..1571462 (+) 465 WP_003333834.1 CtsR family transcriptional regulator -
  D8Z77_RS07335 (D8Z77_07335) - 1571540..1572076 (+) 537 WP_003333833.1 UvrB/UvrC motif-containing protein -
  D8Z77_RS07340 (D8Z77_07340) - 1572103..1573167 (+) 1065 WP_003345125.1 protein arginine kinase -
  D8Z77_RS07345 (D8Z77_07345) - 1573195..1575657 (+) 2463 WP_022585126.1 ATP-dependent Clp protease ATP-binding subunit -
  D8Z77_RS07350 (D8Z77_07350) radA 1575849..1577213 (+) 1365 WP_096888201.1 DNA repair protein RadA Machinery gene
  D8Z77_RS07355 (D8Z77_07355) disA 1577223..1578269 (+) 1047 WP_121473234.1 DNA integrity scanning diadenylate cyclase DisA -
  D8Z77_RS07360 (D8Z77_07360) pssA 1578410..1579129 (+) 720 WP_003345131.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  D8Z77_RS07365 (D8Z77_07365) - 1579231..1579623 (-) 393 WP_003345132.1 hypothetical protein -
  D8Z77_RS07370 (D8Z77_07370) - 1579931..1581022 (+) 1092 WP_003333826.1 PIN/TRAM domain-containing protein -
  D8Z77_RS07375 (D8Z77_07375) ispD 1581053..1581754 (+) 702 WP_003333825.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49541.11 Da        Isoelectric Point: 6.5110

>NTDB_id=319115 D8Z77_RS07350 WP_096888201.1 1575849..1577213(+) (radA) [Brevibacillus laterosporus strain Bl-zj]
MSKVKTKFVCQECGYESPKWLGKCPGCNNWNTMVEELERKQAGGRDRGMSTKQKAQPITEVISEEEPRLDTTLGELNRVL
GGGLVLGSLVLVGGDPGIGKSTLLLQTSFALAHQNNKVLYISGEESVKQIKMRADRLGVQTSNLLVVTETDLEQVEEQIT
FVDPDVVIIDSIQTIFHPAVTSAAGSVAQVRECTSHLMRIAKSKGIAIFIVGHVTKEGAIAGPRMLEHMVDAVLYFEGDR
HNTFRILRAVKNRFGSTNEMGIFEMKEKGLAEVENPSELFLAERPIGVAGSTVVASMEGTRPVLVELQALVSPTSFATPR
RMATGVDHQRIAMIMAVLEKRVGLMLQNQDAYVNVAGGVRLDEPAIDLAIAVSIASSFRDHSTNPYDVVIGEIGLTGEVR
GVSRIEQRIREAEKLGFKRVIIPERSIRGLDKPAGIEVIGVKNVEEALQYALRG

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=319115 D8Z77_RS07350 WP_096888201.1 1575849..1577213(+) (radA) [Brevibacillus laterosporus strain Bl-zj]
ATGTCAAAAGTAAAAACAAAGTTTGTGTGTCAGGAATGCGGGTACGAAAGTCCTAAATGGCTGGGCAAATGCCCAGGCTG
TAATAATTGGAATACAATGGTTGAGGAATTAGAGCGTAAACAAGCCGGTGGTAGAGATCGTGGTATGTCCACTAAACAAA
AGGCACAGCCCATCACAGAGGTCATAAGTGAGGAGGAACCACGGTTAGATACCACCTTAGGTGAATTAAACCGTGTCTTA
GGTGGCGGTTTAGTTTTGGGATCGTTGGTGCTTGTTGGGGGAGATCCGGGGATCGGAAAGTCTACCTTACTTCTCCAAAC
TTCCTTTGCTCTTGCCCATCAGAATAATAAAGTGTTGTATATCTCCGGTGAGGAGTCTGTTAAACAAATTAAAATGCGAG
CTGATCGGCTTGGTGTACAAACATCAAATCTGCTAGTAGTGACGGAGACTGATTTGGAGCAGGTGGAGGAGCAGATCACG
TTTGTCGACCCTGATGTGGTTATTATTGACTCGATCCAAACCATTTTTCATCCTGCTGTCACATCCGCAGCAGGGAGTGT
AGCTCAAGTCCGAGAGTGTACCAGCCATCTGATGCGAATAGCAAAAAGTAAAGGTATCGCTATCTTTATTGTCGGACATG
TTACAAAAGAAGGAGCAATCGCTGGACCGCGTATGCTTGAGCATATGGTTGATGCTGTTCTTTACTTTGAAGGAGATCGA
CATAATACCTTCCGTATTTTACGAGCGGTTAAAAATCGATTTGGTTCTACAAATGAAATGGGTATTTTTGAAATGAAAGA
AAAAGGGCTAGCTGAAGTAGAAAACCCATCTGAGCTATTCTTAGCGGAACGACCAATTGGAGTTGCTGGATCAACGGTAG
TAGCAAGTATGGAAGGAACTCGCCCCGTGCTTGTTGAACTACAGGCATTAGTTTCCCCAACCAGCTTTGCTACTCCTCGT
CGAATGGCAACAGGGGTAGACCATCAGCGAATCGCTATGATTATGGCAGTTTTGGAGAAACGAGTAGGCTTAATGCTACA
AAATCAAGATGCCTATGTAAACGTAGCAGGTGGGGTTCGGTTAGATGAGCCTGCTATTGACTTAGCGATTGCGGTAAGCA
TTGCTAGTAGTTTTCGAGATCATTCAACGAACCCATATGATGTAGTGATTGGAGAAATAGGTTTAACAGGCGAAGTCCGC
GGTGTTTCACGAATTGAGCAACGTATACGCGAAGCGGAAAAACTGGGCTTTAAGCGGGTCATTATTCCAGAGAGAAGCAT
ACGAGGACTAGATAAGCCTGCTGGGATTGAGGTAATTGGTGTGAAAAATGTGGAGGAAGCTTTGCAATATGCATTAAGGG
GGTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(340-425)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(68-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

68.777

100

0.694
  radA Streptococcus mitis NCTC 12261

59.111

99.119

0.586
  radA Streptococcus mitis SK321

59.111

99.119

0.586
  radA Streptococcus pneumoniae Rx1

59.111

99.119

0.586
  radA Streptococcus pneumoniae D39

59.111

99.119

0.586
  radA Streptococcus pneumoniae R6

59.111

99.119

0.586
  radA Streptococcus pneumoniae TIGR4

59.111

99.119

0.586

Multiple sequence alignment