Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   D8M16_RS02320 Genome accession   NZ_CP032841
Coordinates   483935..485368 (-) Length   477 a.a.
NCBI ID   WP_003861864.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain C15117     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 478935..490368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D8M16_RS02290 elbB 480468..481121 (+) 654 WP_062933422.1 isoprenoid biosynthesis glyoxalase ElbB -
  D8M16_RS02295 mtgA 481118..481843 (+) 726 WP_047051240.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  D8M16_RS02300 npr 481882..482154 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  D8M16_RS02305 rapZ 482151..483005 (-) 855 WP_003861870.1 RNase adapter RapZ -
  D8M16_RS02310 ptsN 483051..483542 (-) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  D8M16_RS02315 hpf 483625..483912 (-) 288 WP_003861866.1 ribosome hibernation promoting factor -
  D8M16_RS02320 rpoN/rpoN1 483935..485368 (-) 1434 WP_003861864.1 RNA polymerase factor sigma-54 Machinery gene
  D8M16_RS02325 lptB 485416..486141 (-) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  D8M16_RS02330 lptA 486148..486702 (-) 555 WP_015571984.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  D8M16_RS02335 lptC 486671..487246 (-) 576 WP_015571983.1 LPS export ABC transporter periplasmic protein LptC -
  D8M16_RS02340 kdsC 487243..487809 (-) 567 WP_047051238.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  D8M16_RS02345 kdsD 487824..488810 (-) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  D8M16_RS02350 - 488824..489801 (-) 978 WP_003861852.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54099.96 Da        Isoelectric Point: 4.3358

>NTDB_id=319082 D8M16_RS02320 WP_003861864.1 483935..485368(-) (rpoN/rpoN1) [Enterobacter hormaechei strain C15117]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=319082 D8M16_RS02320 WP_003861864.1 483935..485368(-) (rpoN/rpoN1) [Enterobacter hormaechei strain C15117]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAACTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGTGCAGACTACCAGGATGA
TGAACTCCCGGTCTACCAGGGCGAAACCACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTAGAGCTGACCCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTGGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATTGAAGCCGTCCTGAAACGCGTTCAGCGTTTCGACCC
GATTGGCGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAATCACGACTTCCGCACCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCACTTGATCCGCGTCCGGGGCAGTCGATCCAGACCAG
CGAACCGGAATATGTCATTCCTGATGTGCTGGTGAGAAAACACAATGGCCGGTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCACGTAATGACGCTGATAATCAATATATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATCAAGAGTCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCCATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment