Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LP314_RS03330 Genome accession   NZ_CP032757
Coordinates   703689..705068 (+) Length   459 a.a.
NCBI ID   WP_050339194.1    Uniprot ID   F6IVW0
Organism   Lactiplantibacillus pentosus strain DSM 20314     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 698689..710068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LP314_RS03310 (LP314_03310) - 700021..701352 (-) 1332 WP_050339197.1 aminopeptidase C -
  LP314_RS03315 (LP314_03315) rpiA 701428..702111 (-) 684 WP_050339196.1 ribose-5-phosphate isomerase RpiA -
  LP314_RS03320 (LP314_03320) - 702121..702417 (-) 297 WP_003637758.1 GNAT family N-acetyltransferase -
  LP314_RS03325 (LP314_03325) - 703016..703552 (+) 537 WP_050339195.1 dUTP diphosphatase -
  LP314_RS03330 (LP314_03330) radA 703689..705068 (+) 1380 WP_050339194.1 DNA repair protein RadA Machinery gene
  LP314_RS03335 (LP314_03335) - 705087..706292 (+) 1206 WP_056952826.1 PIN/TRAM domain-containing protein -
  LP314_RS03340 (LP314_03340) gltX 707344..708834 (+) 1491 WP_050339298.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50031.33 Da        Isoelectric Point: 7.3885

>NTDB_id=318539 LP314_RS03330 WP_050339194.1 703689..705068(+) (radA) [Lactiplantibacillus pentosus strain DSM 20314]
MAKAKTQFVCSNCGYSTPRYLGRCPNCGEWNTLTEEHFDTKPDRKSRVSFAGKSAKPQRLKEVSLTKTPRVKTKLKEFNR
VLGGGVVPGSLVLIGGDPGIGKSTLLLQVSGQLAETGGSVLYVTGEESAAQVKMRADRLAVSSEDFFLYPETDMSNIRST
IEETHPDFVVIDSVQTMQEPDMDSAIGSVSQIREITAELLQISKTNGITIFIVGHVTKGGAIAGPKILEHMVDTVLYFEG
DLHHTYRILRAVKNRFGSTNELGIFEMRTGGLYEVANPSEIFLEERLKDATGSAIVVSMEGTRPILVEVQALVTPTVFGN
AKRTASGLDHNRVSLIMAVLEKRASLMLQNQDAYLKAAGGVKLDEPAIDLAIAVSVASSYRDQQTRPSDCFVGEVGLTGE
IRRVNRIESRVGEAAKLGFKRIFIPKNNLQGWDIPTGIEVIGVATLKEALQLALQQPNY

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=318539 LP314_RS03330 WP_050339194.1 703689..705068(+) (radA) [Lactiplantibacillus pentosus strain DSM 20314]
GTGGCAAAAGCTAAAACTCAGTTTGTTTGTTCAAACTGCGGTTATTCGACACCGCGATATTTAGGCCGGTGTCCGAACTG
TGGTGAATGGAATACGTTGACGGAAGAACATTTTGATACGAAGCCAGACCGCAAGTCCCGGGTGAGCTTTGCCGGTAAGT
CGGCGAAACCACAACGATTAAAAGAAGTTTCACTGACGAAGACGCCGCGCGTCAAAACCAAGTTGAAAGAGTTCAACCGC
GTTCTAGGTGGTGGGGTCGTTCCTGGTTCCTTGGTGTTAATTGGTGGGGATCCCGGCATCGGGAAGTCGACGCTACTATT
GCAAGTTTCTGGCCAGCTGGCTGAAACGGGGGGCTCTGTGCTATACGTCACGGGGGAAGAAAGTGCTGCGCAAGTTAAGA
TGCGCGCTGACCGTTTGGCCGTTAGTAGCGAAGATTTCTTTTTATATCCTGAAACGGACATGTCAAATATTCGGTCAACG
ATTGAAGAGACTCATCCGGACTTTGTCGTGATCGACTCGGTTCAAACGATGCAGGAGCCAGATATGGATTCAGCGATTGG
TTCCGTTTCCCAGATTCGAGAAATTACGGCAGAATTGTTGCAAATTTCCAAGACCAACGGCATTACGATTTTTATCGTCG
GTCACGTGACTAAGGGCGGCGCGATCGCCGGTCCAAAGATTTTGGAACACATGGTGGATACGGTGCTGTACTTTGAAGGT
GACCTGCACCACACTTATCGGATTTTACGTGCGGTTAAGAACCGGTTTGGCTCCACAAATGAACTCGGTATTTTTGAAAT
GCGGACGGGGGGACTTTACGAAGTTGCTAATCCATCCGAAATCTTCTTGGAAGAGCGGCTCAAAGATGCGACTGGTTCAG
CCATTGTCGTTTCAATGGAAGGGACTCGGCCAATCTTGGTGGAAGTCCAGGCGCTGGTCACGCCAACGGTATTTGGTAAC
GCCAAGCGGACAGCGAGTGGTTTGGACCACAACCGGGTCTCGCTTATCATGGCGGTACTGGAAAAGCGTGCCAGCTTGAT
GCTTCAAAATCAAGATGCCTATTTGAAGGCCGCTGGTGGCGTCAAACTAGATGAACCAGCGATTGATTTGGCGATTGCCG
TCAGTGTAGCTTCCAGTTATCGCGACCAACAAACGCGTCCGAGTGACTGTTTTGTCGGTGAAGTTGGTTTGACTGGTGAA
ATTCGGCGGGTCAATCGGATCGAATCTCGGGTCGGTGAGGCCGCTAAGTTAGGCTTCAAACGTATTTTTATTCCGAAGAA
TAATCTGCAAGGCTGGGATATTCCAACGGGCATCGAAGTGATTGGGGTGGCGACCTTAAAGGAAGCGCTCCAGTTGGCAT
TACAACAACCAAATTACTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(362-428)

AAA domain (AAA_25)

(59-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F6IVW0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

67.627

98.257

0.664
  radA Streptococcus pneumoniae D39

67.627

98.257

0.664
  radA Streptococcus pneumoniae R6

67.627

98.257

0.664
  radA Streptococcus pneumoniae TIGR4

67.627

98.257

0.664
  radA Streptococcus mitis SK321

67.627

98.257

0.664
  radA Streptococcus mitis NCTC 12261

67.627

98.257

0.664
  radA Bacillus subtilis subsp. subtilis str. 168

65.57

99.346

0.651

Multiple sequence alignment