Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CFBP5499_RS00045 Genome accession   NZ_CP039888
Coordinates   7530..9074 (-) Length   514 a.a.
NCBI ID   WP_080826741.1    Uniprot ID   -
Organism   Agrobacterium tumefaciens strain CFBP5499     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2530..14074
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFBP5499_RS00015 (CFBP5499_00015) rdgB 2742..3386 (-) 645 WP_080826747.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  CFBP5499_RS00020 (CFBP5499_00020) rph 3404..4120 (-) 717 WP_080826746.1 ribonuclease PH -
  CFBP5499_RS00025 (CFBP5499_00025) hrcA 4257..5348 (+) 1092 WP_080826745.1 heat-inducible transcriptional repressor HrcA -
  CFBP5499_RS00030 (CFBP5499_00030) grpE 5462..6097 (+) 636 WP_080826744.1 nucleotide exchange factor GrpE -
  CFBP5499_RS00035 (CFBP5499_00035) ptsN 6180..6641 (-) 462 WP_080826743.1 PTS IIA-like nitrogen regulatory protein PtsN -
  CFBP5499_RS00040 (CFBP5499_00040) raiA 6724..7296 (-) 573 WP_080826742.1 ribosome-associated translation inhibitor RaiA -
  CFBP5499_RS00045 (CFBP5499_00045) rpoN/rpoN1 7530..9074 (-) 1545 WP_080826741.1 RNA polymerase factor sigma-54 Machinery gene
  CFBP5499_RS00050 (CFBP5499_00050) lptB 9228..10013 (-) 786 WP_175416749.1 LPS export ABC transporter ATP-binding protein -
  CFBP5499_RS00055 (CFBP5499_00055) - 10047..10607 (-) 561 WP_175416750.1 LptA/OstA family protein -
  CFBP5499_RS00060 (CFBP5499_00060) lptC 10624..11289 (-) 666 WP_080826738.1 LPS export ABC transporter periplasmic protein LptC -
  CFBP5499_RS00065 (CFBP5499_00065) sppA 11524..12477 (+) 954 WP_080826737.1 signal peptide peptidase SppA -
  CFBP5499_RS00070 (CFBP5499_00070) - 12504..12806 (+) 303 WP_003493505.1 integration host factor subunit beta -
  CFBP5499_RS00075 (CFBP5499_00075) - 12875..13216 (+) 342 WP_175416561.1 LapA family protein -

Sequence

Protein


Download         Length: 514 a.a.        Molecular weight: 56608.92 Da        Isoelectric Point: 4.4553

>NTDB_id=318430 CFBP5499_RS00045 WP_080826741.1 7530..9074(-) (rpoN/rpoN1) [Agrobacterium tumefaciens strain CFBP5499]
MALSASLLLRQNQSLVMTPQLMQSIQLLQMTHFELTQFIAQEVERNPLLEIAANDGDLGSDTPVDVDNFGDGEVSDAAAS
VTPDSDDWYGERAANLGEQLDTSFENVFPDDGEPRKADAPELASQWKSMPGQESGESYDLDDFVAARQSLSDHLNQQLPL
AISASEDRMIADALIGQLDETGYIAADAVDDVAERLGAAPLAVEHVLKTLQGFDPPGIFSRSLSECLAIQLAQKDRLDPA
MRAFVDNLELLAKRDFASLKKLCGVDEEDLLDMLAEIRTLNPRPGAGYDSMVSETIVPDIIVRPSAAGGWLVEINPDTLP
RVLINQSYFAEVSKHKARAGEDQDFLSECMQTAHWLTRSLDQRARTIMKVATEIVRQQDAFLVNGVDQLRPLNLKTVADA
IKMHESTVSRVTSKKYMLTPRGLFELKYFFSVSISAVEGGDSHSAEAVRHRIKAMIAQEAAEAVLSDDDIVDNLKKSGID
IARRTVAKYREAMNIPSSVQRRREKKAMAKLSAF

Nucleotide


Download         Length: 1545 bp        

>NTDB_id=318430 CFBP5499_RS00045 WP_080826741.1 7530..9074(-) (rpoN/rpoN1) [Agrobacterium tumefaciens strain CFBP5499]
ATGGCATTATCTGCCAGCCTTTTGCTGCGTCAAAACCAGTCTCTCGTGATGACACCGCAACTGATGCAGTCCATCCAGCT
GCTTCAGATGACGCATTTCGAGCTGACGCAGTTCATCGCGCAGGAGGTGGAGCGCAATCCGCTGCTGGAAATTGCGGCAA
ACGATGGCGATTTGGGCAGCGATACGCCTGTAGATGTCGATAATTTCGGCGACGGTGAAGTTTCGGATGCGGCCGCCAGC
GTGACGCCCGACAGCGATGACTGGTACGGGGAGCGCGCCGCAAATCTTGGCGAGCAGCTGGATACCAGCTTCGAAAACGT
ATTTCCCGACGATGGCGAACCGAGAAAGGCCGATGCGCCCGAACTCGCGAGCCAGTGGAAATCCATGCCCGGACAGGAGT
CCGGAGAAAGCTACGACCTCGACGACTTCGTTGCGGCCCGACAAAGCCTCAGCGACCACCTCAATCAGCAACTGCCGCTC
GCCATTTCCGCCTCCGAGGACAGGATGATTGCTGATGCGCTGATCGGCCAGCTCGATGAGACGGGTTATATCGCCGCAGA
TGCCGTCGATGACGTCGCTGAACGGCTGGGTGCCGCCCCATTGGCGGTCGAGCACGTTCTAAAGACGCTTCAGGGCTTTG
ATCCGCCCGGCATCTTTTCCCGTTCCTTGAGCGAATGTCTGGCGATCCAGCTCGCGCAGAAGGATCGGCTCGACCCGGCC
ATGCGGGCTTTTGTCGATAATCTCGAGCTTCTGGCGAAACGCGATTTCGCCTCCTTAAAAAAACTCTGCGGGGTCGATGA
AGAAGATCTTCTCGATATGCTGGCGGAAATCCGAACGCTCAATCCGCGTCCCGGCGCAGGTTACGATTCGATGGTTTCGG
AGACCATCGTCCCAGATATCATCGTCCGACCCTCCGCCGCCGGCGGCTGGCTGGTGGAGATCAATCCAGACACCCTGCCG
CGCGTGCTCATCAACCAGAGCTACTTCGCGGAGGTTTCAAAACACAAGGCGCGCGCAGGAGAGGATCAGGATTTTCTGTC
CGAATGCATGCAGACAGCCCATTGGCTGACCCGCAGCCTAGACCAGCGCGCCCGGACGATCATGAAGGTGGCGACAGAAA
TCGTGCGCCAGCAGGACGCCTTCCTCGTCAACGGCGTCGATCAGCTGCGCCCGCTGAACCTCAAAACGGTGGCCGACGCC
ATCAAGATGCATGAATCCACCGTCAGCCGGGTGACATCGAAGAAATACATGCTGACGCCGCGCGGGCTGTTCGAACTTAA
ATATTTCTTCAGCGTGTCCATCAGCGCCGTGGAAGGCGGCGACAGCCATTCGGCGGAAGCGGTGCGTCACCGCATCAAGG
CGATGATCGCACAGGAAGCCGCTGAGGCTGTTCTTTCCGACGACGATATCGTCGACAATCTGAAAAAGAGCGGCATCGAT
ATCGCACGCCGCACCGTCGCCAAATATCGCGAGGCGATGAATATCCCCTCCTCCGTACAGAGGCGCCGGGAGAAGAAAGC
GATGGCGAAGCTTTCCGCCTTCTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-503)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(141-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(7-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

35.97

100

0.372

Multiple sequence alignment