Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AFLV_RS00475 Genome accession   NC_011567
Coordinates   93535..94929 (+) Length   464 a.a.
NCBI ID   WP_012573839.1    Uniprot ID   B7GJ40
Organism   Anoxybacillus flavithermus WK1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 88535..99929
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AFLV_RS00455 (Aflv_0074) - 88936..89397 (+) 462 WP_012573835.1 CtsR family transcriptional regulator -
  AFLV_RS00460 (Aflv_0075) - 89412..89954 (+) 543 WP_035020791.1 UvrB/UvrC motif-containing protein -
  AFLV_RS00465 (Aflv_0076) - 89959..91023 (+) 1065 WP_041637575.1 protein arginine kinase -
  AFLV_RS00470 (Aflv_0077) clpC 91044..93476 (+) 2433 WP_041637576.1 ATP-dependent protease ATP-binding subunit ClpC -
  AFLV_RS00475 (Aflv_0078) radA 93535..94929 (+) 1395 WP_012573839.1 DNA repair protein RadA Machinery gene
  AFLV_RS00480 (Aflv_0079) - 95019..96107 (+) 1089 WP_041637578.1 PIN/TRAM domain-containing protein -
  AFLV_RS00485 (Aflv_0080) ispD 96127..96813 (+) 687 WP_041637580.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  AFLV_RS00490 (Aflv_0081) ispF 96813..97295 (+) 483 WP_041637582.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  AFLV_RS00495 (Aflv_0082) gltX 97359..98819 (+) 1461 WP_012573843.1 glutamate--tRNA ligase -
  AFLV_RS00500 (Aflv_0083) cysE 99134..99799 (+) 666 WP_041637584.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 51072.04 Da        Isoelectric Point: 7.5474

>NTDB_id=31822 AFLV_RS00475 WP_012573839.1 93535..94929(+) (radA) [Anoxybacillus flavithermus WK1]
MVKRKTKFVCQHCGYETAKWMGKCPGCHSWNAMVEETEIVKPHTRALFVHTEEIVAKPVSITTVETTQEPRIDTKFTEFN
RVLGGGIVRGSLVLIGGDPGIGKSTLLLQICAQLASDAHSVLYISGEESVKQTKLRAERLHVTAEHLYVLSETNLEHIIQ
TVDEMKPAFVVIDSIQTIYRSEITSAPGSVSQVRECTAELMRLAKTKGIAIFIVGHVTKEGALAGPRILEHMVDTVLYFE
GERHHTYRILRAVKNRFGSTNEIGIFEMKEMGLTEVKNPSEIFLEERSKGAAGSTVVASMEGTRPVLVEIQALVSPTSFG
TPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGIKLDEPAIDLAIAVSIASSFRDQPTNHTDVVIGEVGLTG
EVRRVSRIEQRVQEAAKLGFQRIIIPKSNIGGWNIPKGVEVIGVINVEEALYYALGEGKNRPIF

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=31822 AFLV_RS00475 WP_012573839.1 93535..94929(+) (radA) [Anoxybacillus flavithermus WK1]
ATGGTTAAGCGGAAAACAAAGTTCGTTTGCCAACATTGTGGATATGAAACGGCGAAATGGATGGGAAAATGTCCGGGGTG
CCATTCGTGGAATGCGATGGTTGAAGAAACAGAAATAGTAAAACCACACACTCGAGCATTATTTGTGCATACAGAAGAGA
TCGTAGCAAAACCCGTATCGATTACAACGGTGGAAACAACGCAGGAGCCGAGAATTGATACGAAGTTTACAGAATTTAAC
CGCGTGTTAGGGGGCGGCATTGTTCGTGGTTCTCTCGTTTTGATTGGGGGGGATCCGGGTATTGGGAAATCGACGTTGTT
GCTACAAATTTGCGCTCAACTCGCAAGCGATGCCCACTCTGTTTTATATATTTCCGGGGAAGAATCGGTAAAACAAACGA
AGTTGCGTGCTGAACGGTTGCATGTGACAGCGGAACATTTATACGTTTTATCTGAAACAAACTTAGAACATATTATCCAA
ACGGTTGATGAGATGAAGCCAGCGTTTGTCGTTATTGATTCCATCCAAACGATATATCGATCAGAAATTACATCGGCCCC
AGGGAGCGTTTCACAAGTTCGTGAATGTACGGCAGAGTTAATGAGACTAGCTAAAACAAAAGGTATTGCAATTTTTATAG
TTGGGCACGTTACAAAAGAAGGAGCGCTTGCAGGACCGCGTATTTTAGAACATATGGTTGATACTGTATTATATTTTGAG
GGAGAGCGTCATCATACGTATCGTATTTTACGGGCAGTGAAAAATCGATTCGGTTCCACAAACGAAATCGGTATTTTCGA
AATGAAAGAAATGGGGCTTACCGAAGTCAAAAACCCATCAGAAATTTTTTTAGAAGAGCGTTCGAAAGGTGCAGCTGGGT
CAACGGTCGTCGCTTCCATGGAAGGAACGAGGCCGGTGTTAGTGGAAATTCAAGCGTTAGTATCACCGACTAGTTTTGGA
ACACCGCGTCGAATGGCGACAGGAATAGACCATAATCGCGTATCTCTTCTTATGGCTGTGCTTGAAAAAAGGGTCGGTCT
ACTTCTTCAAAACCAAGATGCTTATTTAAAGGTAGCTGGTGGCATAAAGTTAGATGAACCTGCGATTGACTTAGCGATTG
CGGTAAGCATTGCATCTAGTTTTCGTGATCAACCAACAAATCATACGGATGTCGTTATTGGGGAAGTCGGATTGACAGGT
GAGGTGCGTCGTGTCTCCCGCATTGAACAGCGTGTACAAGAAGCTGCTAAGCTTGGTTTTCAGCGAATCATTATTCCGAA
AAGCAACATCGGTGGGTGGAACATTCCAAAAGGCGTCGAAGTTATTGGAGTAATCAACGTAGAAGAAGCGCTATACTACG
CGCTAGGGGAGGGGAAAAATCGCCCAATATTTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

AAA domain (AAA_25)

(67-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7GJ40

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

75.93

98.491

0.748
  radA Streptococcus pneumoniae Rx1

62.749

97.198

0.61
  radA Streptococcus pneumoniae D39

62.749

97.198

0.61
  radA Streptococcus pneumoniae R6

62.749

97.198

0.61
  radA Streptococcus pneumoniae TIGR4

62.749

97.198

0.61
  radA Streptococcus mitis NCTC 12261

62.749

97.198

0.61
  radA Streptococcus mitis SK321

62.528

97.198

0.608

Multiple sequence alignment