Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   D8L98_RS02920 Genome accession   NZ_CP032682
Coordinates   589336..590682 (-) Length   448 a.a.
NCBI ID   WP_000816499.1    Uniprot ID   Q9R3W9
Organism   Staphylococcus pseudintermedius strain G3C4     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 575876..590682 589336..590682 within 0


Gene organization within MGE regions


Location: 575876..590682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D8L98_RS02835 (D8L98_02835) mecA 575876..577885 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  D8L98_RS02840 (D8L98_02840) mecR1 577982..579739 (+) 1758 WP_000952923.1 beta-lactam sensor/signal transducer MecR1 -
  D8L98_RS02845 (D8L98_02845) mecI 579739..580110 (+) 372 WP_025904161.1 mecA-type methicillin resistance repressor MecI -
  D8L98_RS02850 (D8L98_02850) psm-mec 580195..580263 (-) 69 WP_014532405.1 phenol-soluble modulin PSM-mec -
  D8L98_RS02855 (D8L98_02855) - 580583..581731 (+) 1149 WP_075773656.1 ROK family transcriptional regulator -
  D8L98_RS02865 (D8L98_02865) cstB 581845..583179 (-) 1335 Protein_532 persulfide dioxygenase-sulfurtransferase CstB -
  D8L98_RS02870 (D8L98_02870) - 583212..584276 (-) 1065 WP_000438836.1 DsrE/DsrF/DrsH-like family protein -
  D8L98_RS02875 (D8L98_02875) cstR 584412..584672 (+) 261 WP_000220507.1 persulfide-sensing transcriptional repressor CstR -
  D8L98_RS02880 (D8L98_02880) - 584672..585310 (+) 639 Protein_535 sulfite exporter TauE/SafE family protein -
  D8L98_RS13395 (D8L98_02885) - 585364..585729 (-) 366 Protein_536 JAB domain-containing protein -
  D8L98_RS02890 (D8L98_02890) - 585722..585928 (-) 207 WP_001077283.1 hypothetical protein -
  D8L98_RS02895 (D8L98_02895) - 585930..586451 (-) 522 WP_000836008.1 DUF1643 domain-containing protein -
  D8L98_RS02900 (D8L98_02900) - 586470..586781 (-) 312 WP_000833212.1 DUF960 family protein -
  D8L98_RS02910 (D8L98_02910) - 586866..587216 (-) 351 WP_000171449.1 SAUGI family uracil-DNA glycosylase inhibitor -
  D8L98_RS02915 (D8L98_02915) ccrB 587687..589315 (-) 1629 WP_001186596.1 recombinase family protein Machinery gene
  D8L98_RS02920 (D8L98_02920) ccrA 589336..590682 (-) 1347 WP_000816499.1 recombinase family protein Machinery gene

Sequence

Protein


Download         Length: 448 a.a.        Molecular weight: 52138.19 Da        Isoelectric Point: 10.1760

>NTDB_id=318183 D8L98_RS02920 WP_000816499.1 589336..590682(-) (ccrA) [Staphylococcus pseudintermedius strain G3C4]
MKRAIGYLRQSTTKQQSLPAQKQAIELLAPKHNIQNIQYISDKQSGRTDNRTGYQQVTERIQQRQCDVLCCYRLNRLHRN
LKNALKLMKLCQKYHVHILSVHDGYFDMDKAFDRLKLNIFMSLAELESDNIGEQVKNGLREKAKQGKLITTHAPFGYHYQ
NGTFIINNDESPTVKAVFNYYLQGYGYKKIAQYLEDDNKLITRKPYQVRNIIMNPNYCGRVINQYGQYNNMVPPIVSATK
YEHAQAIRNKKQLHCIPSENQLKQKIKCPCCDSTLTNMTIRKKHTLRYYICPKNMNESRFVCSFKGINAQKLEVQVLATC
QNFFQNQQLYSKINNAIHQRLKKQRVIEAKSTLTQEQLIDKLAKGMIDAESFRKQTHLMNQKHKTISSISDNQLQTSLQK
VIQKSFTLNMLHPYIDEIRITKNKALVGIYFKNEPLNIVNQTSQSSIA

Nucleotide


Download         Length: 1347 bp        

>NTDB_id=318183 D8L98_RS02920 WP_000816499.1 589336..590682(-) (ccrA) [Staphylococcus pseudintermedius strain G3C4]
ATGAAACGAGCCATTGGTTATTTGCGCCAAAGTACAACGAAACAACAATCACTCCCAGCTCAAAAGCAAGCAATAGAATT
ATTAGCTCCAAAGCACAATATTCAAAATATCCAATACATTAGTGATAAGCAATCAGGCAGAACAGATAATCGAACAGGCT
ATCAACAAGTCACCGAACGCATCCAACAAAGACAATGTGACGTATTATGTTGTTATCGCTTGAATCGACTTCATCGCAAC
TTGAAAAATGCATTAAAACTCATGAAACTCTGTCAAAAATATCATGTTCATATTCTAAGTGTTCATGATGGCTATTTTGA
TATGGATAAAGCGTTTGATCGCCTAAAACTCAATATATTCATGAGTCTGGCTGAACTTGAATCCGATAATATTGGAGAAC
AAGTCAAAAATGGACTTAGAGAAAAGGCAAAACAAGGTAAACTCATAACGACCCATGCGCCTTTCGGTTATCACTATCAA
AATGGTACTTTCATCATTAATAATGATGAATCACCTACCGTCAAAGCTGTATTCAATTATTATCTTCAAGGATATGGCTA
CAAGAAGATTGCACAATATTTAGAAGACGATAATAAACTTATTACCCGCAAGCCTTATCAGGTACGAAATATAATTATGA
ACCCAAATTATTGTGGTCGTGTCATCAATCAATATGGTCAATATAACAATATGGTACCACCTATTGTTTCGGCAACGAAA
TATGAACATGCTCAAGCAATCCGTAATAAGAAGCAACTTCACTGTATACCTTCAGAGAATCAGCTGAAACAAAAGATCAA
ATGTCCTTGTTGTGACTCAACACTGACAAATATGACAATAAGAAAAAAACATACATTGCGATATTATATTTGTCCTAAAA
ATATGAATGAATCTCGCTTTGTCTGTTCATTCAAAGGAATAAATGCACAAAAATTAGAAGTTCAAGTCTTAGCTACATGT
CAGAACTTCTTTCAAAACCAACAGCTCTATTCAAAAATTAATAATGCAATTCATCAACGCCTCAAAAAACAAAGAGTGAT
AGAAGCTAAAAGTACGCTAACTCAAGAACAACTGATAGATAAACTTGCCAAAGGTATGATTGATGCTGAATCATTCAGAA
AACAGACTCATTTGATGAATCAAAAGCACAAAACCATATCCTCCATAAGTGATAATCAGTTACAAACATCACTACAAAAG
GTTATACAGAAAAGTTTCACGTTAAACATGCTGCATCCCTATATTGATGAAATTCGCATTACAAAAAATAAAGCCCTTGT
TGGGATCTATTTCAAAAATGAACCATTGAACATTGTGAACCAAACCTCGCAATCATCGATTGCTTAA

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(168-250)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)

Resolvase, N terminal domain (Resolvase)

(4-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9R3W9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

78.174

100

0.783
  ccrA Staphylococcus aureus N315

72.383

100

0.725

Multiple sequence alignment