Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   E8M03_RS08730 Genome accession   NZ_CP039448
Coordinates   1753596..1755869 (-) Length   757 a.a.
NCBI ID   WP_000594983.1    Uniprot ID   -
Organism   Staphylococcus aureus strain VGC1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1748596..1760869
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E8M03_RS08710 (E8M03_08800) - 1749104..1749979 (-) 876 WP_000717807.1 N-acetylmuramoyl-L-alanine amidase -
  E8M03_RS08715 (E8M03_08805) dtd 1749976..1750428 (-) 453 WP_000869979.1 D-aminoacyl-tRNA deacylase -
  E8M03_RS08720 (E8M03_08810) - 1750440..1752629 (-) 2190 WP_001058593.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  E8M03_RS08725 (E8M03_08815) - 1753056..1753574 (-) 519 WP_000364543.1 adenine phosphoribosyltransferase -
  E8M03_RS08730 (E8M03_08820) recJ 1753596..1755869 (-) 2274 WP_000594983.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  E8M03_RS08735 (E8M03_08825) secDF 1756072..1758351 (-) 2280 WP_000749798.1 protein translocase subunit SecDF -
  E8M03_RS08740 (E8M03_08830) yajC 1758626..1758886 (-) 261 WP_001160682.1 preprotein translocase subunit YajC -
  E8M03_RS08745 (E8M03_08835) tgt 1758905..1760044 (-) 1140 WP_001112045.1 tRNA guanosine(34) transglycosylase Tgt -

Sequence

Protein


Download         Length: 757 a.a.        Molecular weight: 85632.75 Da        Isoelectric Point: 4.8041

>NTDB_id=317741 E8M03_RS08730 WP_000594983.1 1753596..1755869(-) (recJ) [Staphylococcus aureus strain VGC1]
MIKPKYKWKLNKPTEYISDELTSKLKLTPIVKKILESKSIIDEQAIESIISDTDINHDSLQLSDMTKTIERIKRAIANDE
KILVYGDYDADGVTSTTILVTTLQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFKALVAIGTIADLVSLTDENRSLV
KQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFIIGPRLNAVGRLDDASLACELLMTDDEEEAAFLAEQVEHFNRERK
DIVATITEEAMAMAEMKVKNGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSIDQVSMFEIL
SAHQELIAKFGGHHMAAGMTMDIDNIESLAEGLNKWMKELSKTTSLDPVKPVDVLLTENDITIKNIRDMNRLRPFGTDFS
RPIFEMDDLSVSSVKAIGQQKNHLKLTLGESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQLIIQDIAMN
EQQILDYRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDLSNSLQQLQFSQLYIVL
QHNHSIYFDGIPNMDVFKKCYKALITKQETNIQKEGMLLCQHLSVKPDTLKFMLKVFLDLNFVTQEDGLIRINQQPDKRS
IDSSKVYQLRQQRMDVEKQLLYQDFSEIKNWIKSQLS

Nucleotide


Download         Length: 2274 bp        

>NTDB_id=317741 E8M03_RS08730 WP_000594983.1 1753596..1755869(-) (recJ) [Staphylococcus aureus strain VGC1]
ATGATTAAACCGAAGTATAAGTGGAAATTAAATAAACCAACTGAATACATAAGTGATGAATTAACATCAAAATTAAAGTT
AACGCCAATCGTAAAAAAAATATTAGAAAGTAAATCGATTATTGATGAACAAGCAATTGAATCCATCATTTCAGATACAG
ATATCAATCATGATTCACTGCAATTGAGCGATATGACTAAGACTATTGAAAGAATTAAAAGAGCAATAGCGAATGATGAG
AAAATCCTAGTTTATGGTGATTACGATGCCGACGGTGTTACCTCAACTACAATTTTAGTGACAACATTACAACTTTTAGG
TGCACAAGTAGGATGGCATATTCCTAACCGCTTTACCGAAGGATATGGACCAAACGAACTAGCATTTAGAAATGCTCATG
ATGAAGGCATAACATTAATCATAACTGTTGATAATGGCATACAAGGTCATAATGAAATTAAGATGGTTCAAGATTTAGGA
GTTGATGTCATTGTAACTGATCATCATGAAATTGGTAGTACATTACCAGAAGCATATGCAATCGTACATCCAATGCATCC
ATCGTTTAATTATCCGTTCCAACAATTGTGTGGTGCAGGTGTTGCGTACAAATTAGCACAAGCATTGATTGAAAATGTAC
CTGATTATTTTAAAGCATTAGTTGCGATAGGTACCATTGCGGATTTAGTATCCTTAACTGATGAAAATCGATCATTAGTG
AAACAAGGATTAAAAGTCTTAAATGATCAATGTCCGACCTCTGTTAAAGCATTGCTAAAAGAAGCGGGATACAATGACAA
TATTGATGAAGAAACAATTGGTTTTATTATTGGACCTAGACTTAATGCTGTAGGTCGTTTAGATGACGCATCATTAGCTT
GCGAATTGTTGATGACTGACGACGAAGAAGAAGCTGCATTTTTAGCAGAACAAGTTGAACATTTTAACCGTGAAAGAAAA
GATATTGTAGCAACTATCACAGAAGAAGCAATGGCAATGGCAGAAATGAAAGTTAAAAATGGAGATTTATTTTTACTTTT
AGCAAAAGAAAATTGGCATGAAGGTGTCTTAGGTATTGTTGCTTCGAAAATTGTCGAAACATTTGCTTTGCCAACATTAA
TTTTAAATATTGATAGGGAGCAAAATCATGCAAAAGGTTCCGCCAGATCAATTGATCAAGTGTCGATGTTTGAAATACTA
TCAGCACACCAAGAGTTAATCGCTAAATTTGGCGGCCATCATATGGCAGCAGGTATGACGATGGATATTGATAATATAGA
ATCGTTAGCTGAAGGTTTAAATAAATGGATGAAAGAATTATCTAAAACAACTTCGTTAGATCCCGTAAAGCCGGTAGATG
TATTATTAACAGAAAATGATATAACGATTAAAAATATTCGTGATATGAATCGTTTAAGACCCTTCGGAACAGATTTTTCT
AGACCTATTTTTGAAATGGATGATTTATCAGTTTCTTCAGTTAAGGCGATAGGTCAACAAAAAAATCATCTAAAATTGAC
GCTTGGTGAAAGTAATATCGCCGCACTATTTTGGCAAAATGGACACTTAGAACCTGAGTTGCAAGATGAACAGCCAATTA
ATATATTAGGATCTGTTCAAATCAACGAATGGAATGGTAATCAATCACCGCAATTAATTATTCAAGATATTGCGATGAAT
GAACAGCAAATATTAGATTATAGAAGTAAACGAAAAAGTTTACCTTTTACAGAAAATGATGAAAATATTGTCGTGCTTAT
TCATCCTAAAAGTGATAAAGTAAATGCGAATGAATATTATTATGGTGAAGAAATTAAACAACAAACTGATAAAGTAGTAT
TAAGAGATTTACCTACGTCGATGGAAGATTTGTCTAATTCCCTGCAACAACTGCAATTTTCTCAACTTTATATAGTTTTG
CAACATAATCATTCGATTTACTTCGATGGTATACCTAATATGGATGTTTTTAAAAAGTGTTATAAAGCATTAATAACTAA
ACAAGAAACAAATATCCAGAAAGAGGGTATGTTATTGTGTCAACATTTAAGTGTGAAACCAGATACACTTAAATTCATGT
TGAAAGTTTTCTTAGACTTAAATTTTGTAACACAAGAAGATGGTTTAATTCGAATCAATCAACAACCTGATAAAAGATCG
ATTGATTCCAGCAAAGTGTATCAATTAAGACAACAACGTATGGATGTTGAAAAGCAATTATTATATCAAGATTTTTCAGA
AATAAAAAATTGGATAAAGTCACAATTGTCGTGA

Domains

Predicted by InterProScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(563-752)

DHHA1 domain (DHHA1)

(312-436)

RecJ OB domain (RecJ_OB)

(452-556)

DHH family (DHH)

(81-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

37.887

100

0.388
  recJ Streptococcus pneumoniae strain D39V

38.058

100

0.383

Multiple sequence alignment