Detailed information    

insolico Bioinformatically predicted

Overview

Name   recN   Type   Machinery gene
Locus tag   CFK27_RS04485 Genome accession   NZ_CP032654
Coordinates   979903..981597 (+) Length   564 a.a.
NCBI ID   WP_050338776.1    Uniprot ID   A0A2S9W3L0
Organism   Lactiplantibacillus pentosus strain ZFM222     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 974903..986597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFK27_RS04455 (CFK27_04455) - 975124..975984 (+) 861 WP_120768472.1 bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase -
  CFK27_RS04460 (CFK27_04460) xseA 975977..977320 (+) 1344 WP_050338779.1 exodeoxyribonuclease VII large subunit -
  CFK27_RS04465 (CFK27_04465) - 977320..977550 (+) 231 WP_003638601.1 exodeoxyribonuclease VII small subunit -
  CFK27_RS04470 (CFK27_04470) - 977543..978433 (+) 891 WP_105921455.1 polyprenyl synthetase family protein -
  CFK27_RS04475 (CFK27_04475) - 978446..979264 (+) 819 WP_120768474.1 TlyA family RNA methyltransferase -
  CFK27_RS04480 (CFK27_04480) - 979348..979806 (+) 459 WP_021336444.1 arginine repressor -
  CFK27_RS04485 (CFK27_04485) recN 979903..981597 (+) 1695 WP_050338776.1 DNA repair protein RecN Machinery gene
  CFK27_RS04490 (CFK27_04490) - 982122..983306 (+) 1185 WP_162255255.1 betaine/proline/choline family ABC transporter ATP-binding protein -
  CFK27_RS04495 (CFK27_04495) - 983303..983932 (+) 630 WP_050338774.1 ABC transporter permease -
  CFK27_RS04500 (CFK27_04500) - 983947..984882 (+) 936 WP_088769611.1 osmoprotectant ABC transporter substrate-binding protein -
  CFK27_RS04505 (CFK27_04505) - 984882..985535 (+) 654 WP_003638609.1 ABC transporter permease -
  CFK27_RS04510 (CFK27_04510) - 985701..986036 (-) 336 WP_082230244.1 hypothetical protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62771.22 Da        Isoelectric Point: 4.9335

>NTDB_id=317623 CFK27_RS04485 WP_050338776.1 979903..981597(+) (recN) [Lactiplantibacillus pentosus strain ZFM222]
MLQELSITNFAIIEHLDIAFEAGMTVLTGETGAGKSIIIDAVGLLAGGRGSSEFIRTGADKAVLQGMFILPADGVTAQLL
DEAGIDHADNTVILQREITKSGRNTCRINGMLVNTTTLKQIGETIVDIHGQNEHQELMQPEKHLGLLDEFAAAKIRKLKQ
QYQQQYDHYQQLNRELRQKNANEKEWAQRLDMLNFQVDEIAAAQVKVGEEAALIAERDRLDNYQMINQALQQSYTLLAAG
EETTGAVDMVGTAMNALEPIANLDSAFNEITVNVKNAFYGLQDAAGQISNQLDLQEFDEGRLDEIEQRLDVLSQLKRKYG
DSEQQILDYYQKIAAELAKMTDSEENSEDLAQRVTDAKDKLLKTGEALSDKRRTAAKVLQRQIHQELKDLYMDKAIFEVR
FKPTKGQIYRSGLDSVEFYIQTNPGERMRPLAKIASGGELSRMMLALKTIFSESQGITSIIFDEVDTGVSGRVAQAIAEK
INGIAHFSQVLCITHLPQVAAMSDHHYFIAKKITGKRTKTSVTKLDNAARVKELARMLAGTKVTKLSLEHAEELLQLADE
EKDE

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=317623 CFK27_RS04485 WP_050338776.1 979903..981597(+) (recN) [Lactiplantibacillus pentosus strain ZFM222]
GTGTTACAAGAACTATCGATTACGAATTTTGCAATTATTGAACATTTAGATATTGCCTTTGAAGCTGGGATGACAGTTTT
GACTGGTGAGACGGGTGCTGGTAAATCGATTATTATTGATGCGGTCGGCCTGTTAGCAGGTGGCCGCGGTTCGTCAGAAT
TTATTCGGACGGGCGCAGATAAAGCTGTCTTACAGGGGATGTTTATTTTGCCGGCGGACGGCGTGACGGCTCAACTGTTA
GATGAAGCAGGAATTGACCACGCTGACAATACGGTGATTTTGCAACGCGAAATTACGAAGAGTGGCCGCAACACTTGTCG
AATCAATGGCATGCTGGTGAATACGACGACTTTGAAGCAAATTGGTGAAACGATTGTTGATATTCATGGACAAAACGAAC
ATCAAGAATTGATGCAACCAGAAAAGCATTTAGGGCTATTAGATGAATTTGCTGCGGCCAAGATTCGGAAGTTAAAACAG
CAGTATCAGCAGCAGTATGATCACTATCAGCAATTAAATCGCGAATTACGACAGAAAAATGCAAATGAAAAAGAATGGGC
ACAACGTTTAGACATGCTGAATTTTCAGGTTGATGAAATCGCAGCTGCCCAAGTTAAAGTTGGTGAAGAGGCGGCGCTGA
TTGCTGAACGGGATCGGCTTGATAACTATCAAATGATCAATCAGGCGTTGCAACAAAGCTATACGTTGTTAGCTGCTGGC
GAGGAAACGACTGGAGCTGTCGACATGGTTGGGACGGCGATGAATGCATTGGAGCCGATTGCCAATTTGGATTCGGCGTT
CAACGAAATTACCGTCAATGTCAAGAATGCCTTTTATGGCTTGCAGGATGCAGCTGGGCAAATCTCAAATCAGTTAGACT
TACAAGAATTCGATGAAGGGCGGCTAGATGAAATTGAGCAACGCCTAGATGTGTTGAGCCAATTGAAACGCAAGTACGGT
GATTCTGAACAGCAAATTCTCGATTATTATCAAAAAATTGCCGCTGAGCTCGCCAAAATGACTGACTCAGAAGAAAATAG
TGAAGATCTCGCACAACGAGTGACCGATGCCAAAGACAAACTGTTGAAGACGGGTGAGGCCTTGTCAGATAAGCGGCGCA
CGGCTGCCAAAGTCCTACAACGTCAGATTCATCAAGAGCTCAAAGATTTATACATGGACAAGGCCATTTTCGAAGTTCGG
TTTAAGCCCACTAAAGGTCAAATCTATCGGAGTGGTTTGGATAGTGTCGAGTTTTACATTCAAACCAACCCAGGTGAGCG
AATGCGACCACTGGCTAAGATTGCCTCTGGTGGGGAACTCTCACGGATGATGCTAGCACTCAAGACGATTTTCTCAGAAT
CACAAGGAATCACCAGTATTATCTTCGACGAAGTGGATACCGGGGTCAGCGGTCGGGTCGCACAAGCGATTGCTGAAAAA
ATCAATGGAATTGCACACTTTTCACAAGTGCTGTGTATCACGCATCTGCCACAAGTAGCGGCAATGAGTGATCATCATTA
CTTCATTGCCAAAAAAATCACCGGCAAACGCACCAAGACGAGTGTGACCAAGTTGGATAACGCTGCCCGGGTGAAAGAAT
TGGCCCGGATGCTGGCTGGTACGAAGGTGACTAAACTTTCATTGGAACATGCCGAGGAGCTATTGCAACTGGCCGATGAA
GAAAAGGACGAATGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(1-511)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S9W3L0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recN Bacillus subtilis subsp. subtilis str. 168

46.724

100

0.48

Multiple sequence alignment