Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   E9097_RS05120 Genome accession   NZ_CP039404
Coordinates   1019969..1020409 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain 377323_2f     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1014969..1025409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E9097_RS05100 (E9097_05100) coaE 1015459..1016079 (-) 621 WP_001269524.1 dephospho-CoA kinase -
  E9097_RS25435 - 1016104..1016148 (+) 45 WP_120795372.1 protein YacM -
  E9097_RS05105 (E9097_05105) guaC 1016304..1017347 (+) 1044 WP_001217338.1 GMP reductase -
  E9097_RS05110 (E9097_05110) hofC 1017382..1018584 (-) 1203 WP_000157242.1 protein transport protein HofC -
  E9097_RS05115 (E9097_05115) pilB 1018574..1019959 (-) 1386 WP_024231534.1 type II secretion system protein GspE Machinery gene
  E9097_RS05120 (E9097_05120) pilA 1019969..1020409 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  E9097_RS05125 (E9097_05125) nadC 1020613..1021506 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  E9097_RS05130 (E9097_05130) ampD 1021594..1022145 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  E9097_RS05135 (E9097_05135) ampE 1022142..1022996 (+) 855 WP_000171984.1 beta-lactamase regulator AmpE -
  E9097_RS05140 (E9097_05140) aroP 1023039..1024412 (-) 1374 WP_072172188.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=317622 E9097_RS05120 WP_000360914.1 1019969..1020409(-) (pilA) [Escherichia coli strain 377323_2f]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=317622 E9097_RS05120 WP_000360914.1 1019969..1020409(-) (pilA) [Escherichia coli strain 377323_2f]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTACAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363