Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CFK27_RS00185 Genome accession   NZ_CP032654
Coordinates   42685..44064 (+) Length   459 a.a.
NCBI ID   WP_050339194.1    Uniprot ID   F6IVW0
Organism   Lactiplantibacillus pentosus strain ZFM222     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 37685..49064
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFK27_RS00165 (CFK27_00165) - 39008..40339 (-) 1332 WP_065674502.1 aminopeptidase C -
  CFK27_RS00170 (CFK27_00170) rpiA 40415..41098 (-) 684 WP_088770021.1 ribose-5-phosphate isomerase RpiA -
  CFK27_RS00175 (CFK27_00175) - 41108..41404 (-) 297 WP_120767938.1 GNAT family N-acetyltransferase -
  CFK27_RS00180 (CFK27_00180) - 42012..42548 (+) 537 WP_050339195.1 dUTP diphosphatase -
  CFK27_RS00185 (CFK27_00185) radA 42685..44064 (+) 1380 WP_050339194.1 DNA repair protein RadA Machinery gene
  CFK27_RS00190 (CFK27_00190) - 44083..45285 (+) 1203 WP_120767940.1 PIN/TRAM domain-containing protein -
  CFK27_RS00195 (CFK27_00195) gltX 46337..47827 (+) 1491 WP_088771263.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50031.33 Da        Isoelectric Point: 7.3885

>NTDB_id=317613 CFK27_RS00185 WP_050339194.1 42685..44064(+) (radA) [Lactiplantibacillus pentosus strain ZFM222]
MAKAKTQFVCSNCGYSTPRYLGRCPNCGEWNTLTEEHFDTKPDRKSRVSFAGKSAKPQRLKEVSLTKTPRVKTKLKEFNR
VLGGGVVPGSLVLIGGDPGIGKSTLLLQVSGQLAETGGSVLYVTGEESAAQVKMRADRLAVSSEDFFLYPETDMSNIRST
IEETHPDFVVIDSVQTMQEPDMDSAIGSVSQIREITAELLQISKTNGITIFIVGHVTKGGAIAGPKILEHMVDTVLYFEG
DLHHTYRILRAVKNRFGSTNELGIFEMRTGGLYEVANPSEIFLEERLKDATGSAIVVSMEGTRPILVEVQALVTPTVFGN
AKRTASGLDHNRVSLIMAVLEKRASLMLQNQDAYLKAAGGVKLDEPAIDLAIAVSVASSYRDQQTRPSDCFVGEVGLTGE
IRRVNRIESRVGEAAKLGFKRIFIPKNNLQGWDIPTGIEVIGVATLKEALQLALQQPNY

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=317613 CFK27_RS00185 WP_050339194.1 42685..44064(+) (radA) [Lactiplantibacillus pentosus strain ZFM222]
GTGGCAAAAGCTAAAACTCAGTTTGTTTGTTCAAACTGCGGTTATTCGACACCGCGATATTTAGGCCGGTGTCCGAACTG
TGGTGAATGGAATACGTTGACGGAAGAACATTTTGATACGAAGCCAGACCGCAAGTCCCGGGTGAGCTTTGCCGGTAAGT
CGGCGAAACCACAACGGTTAAAAGAAGTTTCACTGACGAAGACGCCGCGCGTCAAAACCAAGTTGAAAGAGTTCAACCGC
GTTCTCGGTGGTGGGGTCGTTCCTGGTTCCTTAGTGTTAATTGGTGGGGATCCCGGCATTGGGAAGTCGACGCTACTATT
GCAAGTTTCTGGCCAGTTGGCTGAGACCGGGGGTTCCGTGCTATACGTCACGGGGGAAGAAAGTGCTGCGCAAGTTAAGA
TGCGCGCTGACCGTTTGGCCGTTAGTAGCGAAGATTTCTTTTTATATCCTGAAACGGACATGTCAAATATTCGGTCAACG
ATTGAAGAGACTCATCCGGACTTTGTTGTGATTGACTCGGTTCAAACGATGCAGGAGCCAGATATGGATTCAGCGATTGG
TTCCGTTTCCCAGATTCGAGAAATTACGGCAGAATTATTGCAAATTTCCAAGACCAACGGCATTACGATTTTTATCGTCG
GTCACGTGACTAAGGGCGGGGCGATCGCCGGTCCGAAGATTTTGGAACACATGGTGGATACGGTGCTGTACTTTGAAGGT
GACCTGCACCACACTTATCGGATTTTACGTGCGGTTAAGAACCGGTTTGGCTCCACAAATGAACTCGGTATTTTTGAAAT
GCGGACGGGGGGACTTTACGAAGTTGCCAATCCATCCGAAATCTTCCTGGAAGAACGGCTCAAAGATGCGACTGGTTCAG
CCATCGTCGTTTCAATGGAAGGCACCCGGCCAATCTTGGTGGAAGTCCAGGCGCTGGTCACGCCAACGGTATTTGGTAAC
GCCAAGCGGACGGCGAGTGGTTTGGATCACAACCGGGTCTCGCTTATCATGGCGGTATTGGAAAAACGCGCGAGCTTGAT
GCTTCAAAATCAAGATGCCTATTTGAAGGCCGCTGGTGGGGTCAAATTAGATGAACCAGCGATTGACTTGGCGATTGCCG
TCAGTGTGGCTTCCAGTTATCGCGACCAACAAACGCGTCCGAGTGACTGTTTTGTCGGTGAAGTCGGCTTGACTGGTGAA
ATTCGGCGGGTCAATCGGATCGAATCTCGGGTCGGTGAGGCCGCTAAGTTAGGCTTCAAACGTATTTTTATTCCGAAGAA
TAATCTGCAAGGCTGGGATATTCCGACAGGCATCGAAGTGATTGGGGTGGCGACCTTAAAGGAAGCGCTCCAGTTGGCAT
TACAACAACCAAATTACTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(362-428)

AAA domain (AAA_25)

(59-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F6IVW0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

67.627

98.257

0.664
  radA Streptococcus pneumoniae D39

67.627

98.257

0.664
  radA Streptococcus pneumoniae R6

67.627

98.257

0.664
  radA Streptococcus pneumoniae TIGR4

67.627

98.257

0.664
  radA Streptococcus mitis SK321

67.627

98.257

0.664
  radA Streptococcus mitis NCTC 12261

67.627

98.257

0.664
  radA Bacillus subtilis subsp. subtilis str. 168

65.57

99.346

0.651

Multiple sequence alignment