Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CFK76_RS00425 Genome accession   NZ_CP032652
Coordinates   75120..76484 (+) Length   454 a.a.
NCBI ID   WP_011375373.1    Uniprot ID   A0A094XXJ4
Organism   Latilactobacillus sakei strain LZ217     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 70120..81484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFK76_RS00405 (CFK76_00405) - 70333..71679 (-) 1347 WP_035146635.1 C1 family peptidase -
  CFK76_RS00410 (CFK76_00410) - 71956..73278 (-) 1323 WP_099960399.1 C1 family peptidase -
  CFK76_RS00415 (CFK76_00415) rpiA 73629..74312 (-) 684 WP_016265671.1 ribose-5-phosphate isomerase RpiA -
  CFK76_RS00420 (CFK76_00420) - 74508..75047 (+) 540 WP_016265670.1 deoxyuridine 5'-triphosphate nucleotidohydrolase -
  CFK76_RS00425 (CFK76_00425) radA 75120..76484 (+) 1365 WP_011375373.1 DNA repair protein RadA Machinery gene
  CFK76_RS00430 (CFK76_00430) - 76515..77627 (+) 1113 WP_025015969.1 PIN/TRAM domain-containing protein -
  CFK76_RS00435 (CFK76_00435) cysS 78417..79823 (+) 1407 WP_120760956.1 cysteine--tRNA ligase -
  CFK76_RS00440 (CFK76_00440) - 79820..80227 (+) 408 WP_004265086.1 Mini-ribonuclease 3 -
  CFK76_RS00445 (CFK76_00445) rlmB 80214..81101 (+) 888 WP_011375369.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49598.97 Da        Isoelectric Point: 7.3488

>NTDB_id=317576 CFK76_RS00425 WP_011375373.1 75120..76484(+) (radA) [Latilactobacillus sakei strain LZ217]
MAKVKTQFVCQNCGYSSPRFLGRCPNCGAWNQMVEEREQPAAAAKSNFTISGRATEPEKISTVNIQKEPRVKTELNELNR
VLGGGVVPGSLILIGGDPGIGKSTLLLQVSGQLEKVGKILYVSGEESASQIKMRANRLGVNGDQLYLYPETDMGNIRHQI
ETLKPEYVVIDSIQTMSEPEVTSAVGSVSQVRQVTAELMRIAKTNQITIFVVGHVTKEGAIAGPKILEHMVDTVLYFEGD
THHTYRILRSVKNRFGSTNEIGIFEMREAGLQEVANPSEIFLEERLAGATGSAVVVSMEGTRPILVELQTLITPTLFGNA
KRTSSGLDHNRVSLIMAVLEKRASLMLSNQDAYLKATGGVKLDEPAIDLAMAVSIASSYRDKEIPPTDCFVGEIGLTGEI
RRVNRIEQRVGEAAKLGFKRIYVPKNNLQGWDPPTDIQVVGVTTIAETLKKVFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=317576 CFK76_RS00425 WP_011375373.1 75120..76484(+) (radA) [Latilactobacillus sakei strain LZ217]
GTGGCTAAAGTTAAAACGCAGTTTGTCTGCCAGAATTGTGGCTATAGTTCCCCCAGATTCTTGGGGCGCTGTCCGAATTG
TGGTGCTTGGAACCAAATGGTTGAAGAACGTGAACAACCAGCCGCAGCGGCAAAATCAAATTTCACAATTTCAGGGCGCG
CCACTGAACCTGAAAAAATTAGCACCGTTAATATCCAAAAAGAACCCCGTGTTAAAACGGAATTAAATGAATTGAATCGT
GTCTTAGGCGGCGGTGTGGTGCCGGGGTCACTGATTTTAATCGGTGGGGATCCTGGGATTGGAAAATCAACCTTGTTACT
ACAAGTTTCAGGGCAACTTGAAAAAGTGGGTAAGATTTTATACGTTTCAGGTGAAGAAAGTGCCTCACAAATTAAGATGC
GGGCGAATCGATTAGGGGTTAATGGCGATCAATTGTATCTGTATCCCGAAACGGATATGGGCAATATTCGCCACCAAATT
GAAACGCTCAAACCGGAATACGTCGTGATTGATTCGATTCAAACGATGAGTGAACCAGAAGTGACTTCCGCAGTCGGGAG
TGTTTCGCAAGTTCGCCAAGTAACCGCAGAATTGATGCGGATTGCTAAAACGAATCAAATTACCATCTTCGTGGTGGGAC
ATGTGACGAAGGAAGGGGCGATTGCCGGCCCTAAGATTTTGGAACATATGGTGGATACCGTGTTGTACTTTGAAGGTGAC
ACCCATCACACTTACCGGATTTTACGATCTGTTAAAAACCGGTTTGGTTCAACCAATGAAATCGGGATTTTCGAAATGCG
GGAAGCGGGCTTGCAAGAAGTCGCTAATCCATCCGAAATTTTCCTTGAAGAACGACTAGCCGGTGCGACTGGCTCAGCAG
TTGTGGTTTCAATGGAAGGGACAAGACCAATCTTGGTTGAGTTACAAACTTTGATTACCCCAACCTTGTTCGGGAATGCG
AAACGGACCTCTTCAGGGTTAGATCATAATCGGGTGTCATTGATTATGGCGGTTTTAGAAAAGCGGGCGAGTTTGATGCT
TTCAAACCAAGATGCTTATCTTAAAGCAACCGGTGGCGTTAAGTTAGATGAACCAGCGATTGATTTGGCGATGGCCGTTT
CAATTGCTTCCAGTTATCGCGATAAAGAAATTCCACCAACCGATTGTTTTGTCGGTGAAATTGGCTTGACGGGTGAAATT
CGGCGGGTCAACCGGATCGAACAACGCGTTGGCGAAGCGGCGAAGTTAGGTTTTAAACGGATTTATGTTCCTAAAAATAA
TTTGCAAGGTTGGGATCCACCAACTGATATTCAAGTCGTTGGGGTCACAACTATTGCAGAAACTTTAAAAAAAGTATTCA
ACTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-427)

AAA domain (AAA_25)

(70-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A094XXJ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

69.316

99.78

0.692
  radA Streptococcus mitis SK321

69.316

99.78

0.692
  radA Streptococcus pneumoniae Rx1

69.095

99.78

0.689
  radA Streptococcus pneumoniae D39

69.095

99.78

0.689
  radA Streptococcus pneumoniae R6

69.095

99.78

0.689
  radA Streptococcus pneumoniae TIGR4

69.095

99.78

0.689
  radA Bacillus subtilis subsp. subtilis str. 168

65.121

99.78

0.65

Multiple sequence alignment