Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CN228_RS04265 Genome accession   NZ_CP032631
Coordinates   839824..841191 (+) Length   455 a.a.
NCBI ID   WP_003381736.1    Uniprot ID   Q0EDX1
Organism   Pseudomonas syringae pv. actinidiae str. Shaanxi_M228     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 834824..846191
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CN228_RS04235 (CN228_04235) cydB 835113..836120 (+) 1008 WP_003381747.1 cytochrome d ubiquinol oxidase subunit II -
  CN228_RS04240 (CN228_04240) - 836142..836309 (+) 168 WP_003381744.1 DUF2474 domain-containing protein -
  CN228_RS04245 (CN228_04245) - 836397..837521 (-) 1125 WP_003381743.1 class I SAM-dependent methyltransferase -
  CN228_RS04250 (CN228_04250) - 837651..837884 (-) 234 WP_003381740.1 helix-turn-helix transcriptional regulator -
  CN228_RS04255 (CN228_04255) - 838222..838998 (+) 777 WP_003381739.1 ferredoxin--NADP reductase -
  CN228_RS04260 (CN228_04260) mscL 839183..839629 (-) 447 WP_003381737.1 large-conductance mechanosensitive channel protein MscL -
  CN228_RS04265 (CN228_04265) radA 839824..841191 (+) 1368 WP_003381736.1 DNA repair protein RadA Machinery gene
  CN228_RS04270 (CN228_04270) - 841212..841580 (-) 369 WP_003381735.1 PilZ domain-containing protein -
  CN228_RS04275 (CN228_04275) - 841867..843942 (+) 2076 WP_017683110.1 carbon starvation CstA family protein -
  CN228_RS04280 (CN228_04280) - 843970..844167 (+) 198 WP_002555232.1 YbdD/YjiX family protein -
  CN228_RS04285 (CN228_04285) yjiA 844263..845240 (+) 978 WP_024533195.1 GTPase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48839.40 Da        Isoelectric Point: 7.1315

>NTDB_id=317345 CN228_RS04265 WP_003381736.1 839824..841191(+) (radA) [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
MAKAKRLYGCTECGATFPKWAGQCADCGAWNTLVETMIESGAAAPPSGRTGWTGSQAQIRTLAEVSVEEIPRFSTNSAEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAQRMPALYVTGEESQQQVAMRARRLGLPQDKLRVMTETCIETIIA
TAKVEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHMS
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPPGLQIIAVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=317345 CN228_RS04265 WP_003381736.1 839824..841191(+) (radA) [Pseudomonas syringae pv. actinidiae str. Shaanxi_M228]
ATGGCCAAGGCCAAACGCTTGTACGGCTGCACCGAGTGCGGCGCGACATTTCCCAAGTGGGCGGGCCAGTGTGCTGATTG
CGGCGCCTGGAACACACTGGTCGAAACCATGATAGAAAGCGGTGCCGCAGCGCCTCCCAGCGGGCGCACCGGCTGGACCG
GCTCACAGGCGCAGATCAGAACCCTGGCTGAAGTCAGCGTTGAAGAGATCCCGCGGTTTTCCACCAACTCCGCCGAACTC
GACAGAGTGCTCGGTGGCGGTTTGGTGGATGGTTCTGTGGTGCTGATCGGCGGCGACCCTGGCATCGGCAAGTCGACAAT
TCTGTTGCAAACGCTGTGTAACATCGCCCAGCGCATGCCTGCGCTGTATGTCACCGGCGAGGAATCCCAACAGCAGGTGG
CCATGCGCGCTCGCCGTCTGGGCCTGCCGCAGGACAAACTGCGGGTCATGACCGAAACCTGCATCGAAACCATTATTGCC
ACCGCCAAGGTCGAGAAGCCCAAGGTAATGGTGATCGACTCGATCCAGACGATCTTCACCGAGCAATTGCAGTCCGCACC
CGGTGGCGTGTCGCAAGTGCGCGAAAGCGCCGCCTTGCTGGTGCGCTACGCCAAGCAGAGTGGCACCGCGATTTTCCTGG
TGGGGCATGTCACCAAGGAAGGCGCACTGGCGGGGCCGCGTGTGCTTGAGCACATGGTCGATACCGTGCTGTATTTCGAA
GGCGAGTCGGACGGTCGTCTGCGTTTGCTGCGCGCCGTAAAGAACCGTTTCGGCGCGGTAAACGAACTGGGCGTGTTCGG
CATGACCGACAAGGGCCTGAAAGAAGTGTCCAATCCGTCGGCGATTTTTCTGACCCGTGCGCAGGAAGAAGTGCCCGGCA
GCGTGGTCATGGCCACATGGGAAGGCACCCGACCGATGCTGGTCGAAGTTCAGGCGCTGGTGGATGACAGCCATATGTCC
AATCCGCGTCGTGTAACCCTGGGCCTGGATCAGAACCGTCTGGCGATGCTGCTGGCGGTGCTGCACCGGCATGGCGGCAT
TCCGACCCACGACCAGGACGTGTTCCTCAACGTGGTGGGCGGGGTCAAAGTGCTGGAAACCGCGTCGGACCTGGCCCTGA
TGGCCGCTGTCATGTCCAGCCTGCGCAATCGCCCGCTGCCGCATGACTTGCTGGTGTTTGGCGAGGTGGGGCTGTCGGGT
GAGGTCAGGCCGGTGCCCAGCGGGCAGGAGCGCTTGAAAGAGGCCGCCAAGCACGGCTTCAAACGGGCCATCGTGCCGAA
AGGCAACGCGCCGAAAGAAGCGCCTCCCGGTCTGCAGATCATTGCCGTAACGCGTCTGGAACAGGCGCTGGATGCTTTGT
TCGAGTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)

AAA domain (AAA_25)

(73-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0EDX1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.123

100

0.492
  radA Streptococcus pneumoniae Rx1

46.739

100

0.473
  radA Streptococcus pneumoniae R6

46.739

100

0.473
  radA Streptococcus pneumoniae TIGR4

46.739

100

0.473
  radA Streptococcus pneumoniae D39

46.739

100

0.473
  radA Streptococcus mitis SK321

46.93

100

0.47
  radA Streptococcus mitis NCTC 12261

46.711

100

0.468

Multiple sequence alignment