Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   D7I46_RS08345 Genome accession   NZ_CP032627
Coordinates   1813048..1814409 (-) Length   453 a.a.
NCBI ID   WP_120772482.1    Uniprot ID   A0A387BIA3
Organism   Lactococcus allomyrinae strain 1JSPR-7     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1808048..1819409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D7I46_RS08335 (D7I46_08335) - 1808275..1808601 (-) 327 WP_120772480.1 LPXTG cell wall anchor domain-containing protein -
  D7I46_RS08340 (D7I46_08340) - 1808616..1812740 (-) 4125 WP_120772481.1 beta strand repeat-containing protein -
  D7I46_RS08345 (D7I46_08345) radA 1813048..1814409 (-) 1362 WP_120772482.1 DNA repair protein RadA Machinery gene
  D7I46_RS08355 (D7I46_08355) - 1815030..1815896 (-) 867 WP_120772483.1 helix-turn-helix transcriptional regulator -
  D7I46_RS08360 (D7I46_08360) - 1816042..1816524 (+) 483 WP_120772484.1 AraC family transcriptional regulator -
  D7I46_RS08365 (D7I46_08365) rplQ 1816786..1817166 (-) 381 WP_120772485.1 50S ribosomal protein L17 -
  D7I46_RS08370 (D7I46_08370) - 1817182..1818120 (-) 939 WP_120772486.1 DNA-directed RNA polymerase subunit alpha -
  D7I46_RS08375 (D7I46_08375) rpsK 1818171..1818554 (-) 384 WP_010906297.1 30S ribosomal protein S11 -
  D7I46_RS08380 (D7I46_08380) rpsM 1818575..1818940 (-) 366 WP_120772487.1 30S ribosomal protein S13 -
  D7I46_RS08385 (D7I46_08385) rpmJ 1818958..1819074 (-) 117 WP_014024193.1 50S ribosomal protein L36 -
  D7I46_RS08390 (D7I46_08390) infA 1819128..1819346 (-) 219 WP_003130554.1 translation initiation factor IF-1 -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49967.30 Da        Isoelectric Point: 5.0005

>NTDB_id=317310 D7I46_RS08345 WP_120772482.1 1813048..1814409(-) (radA) [Lactococcus allomyrinae strain 1JSPR-7]
MAKKKSSFICQNCGYKSATYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPMKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVMYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRSEIE
HLKPNFLIIDSIQTIMTPEVQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLIEVNNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRAGLLMQNQDAYLKSAGGVKLDEPAIDLAVAIAVASSYKELSTDARECFIGEIGLTGEIR
RVTRIEQRINEAAKLGFKKVYAPKNSISGIEIPENIEVVGVTTLMECMKLVFS

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=317310 D7I46_RS08345 WP_120772482.1 1813048..1814409(-) (radA) [Lactococcus allomyrinae strain 1JSPR-7]
ATGGCTAAGAAAAAATCATCCTTTATTTGTCAAAACTGTGGCTATAAATCGGCAACATACCTCGGACGATGTCCAAACTG
TGGAGAATGGTCTTCATTTGTTGAAGAAGTTGAAGTCCAAGAAGTTAAAAACCAAAGAGTCTCAATGACTGGTGAACGTT
CAAAACCGATGAAACTAGATGAAGTTGAACTTTTTGACACACCAAGAATTGAGACAGATTTAGACGAGTTTAATCGTGTA
CTTGGTGGAGGTGTTGTACCAGGTTCTTTAGTCTTAATCGGTGGAGACCCAGGGATTGGTAAATCAACATTGCTTCTGCA
AGTATCCACACAGTTGGCATCACGTGGACGTGTCATGTATGTCAGTGGTGAGGAGTCTGCTCAGCAGATTAAACTACGAG
CAGAGCGGCTTGGAGATATTGATAGAGATTTTTATCTTTATGCTGAAACAAATATGCAGTCTATTCGCTCAGAAATAGAA
CATTTGAAACCCAATTTTCTGATTATTGACTCTATCCAGACGATTATGACACCAGAGGTACAGTCTACACAAGGATCAGT
AAGTCAAGTACGTGAAGTGACAGGAGAGCTGATGCAGATTGCTAAAACCAATGATATTGCAACTTTCATCGTTGGCCATG
TCACAAAAGAAGGACAGCTTGCAGGGCCTCGGATGCTTGAGCATATGGTAGATACAGTCTTATATTTTGAAGGCGAAAGG
AATAACACTTTTAGGATTTTGCGTGCAGTAAAAAACCGTTTTGGCTCAACCAATGAAATAGGGATTTTTGAGATGCAAGG
ACATGGTTTGATTGAAGTTAATAATCCTTCGGAAGTTTTTCTAGAAGAACGCTTAGAAGGCTCAACAGGTTCTGCGATTG
TTTGTGCACTTGAGGGGACACGACCTATCTTGGTAGAAATTCAGGCATTGACCACACCAACGATGTTTGGCAACGCCAAA
CGAACGACATCTGGACTTGATTTCAACCGTGTAAGTTTGATTATGGCAGTTTTAGAAAAACGTGCAGGTCTGCTCATGCA
AAATCAAGATGCTTATCTGAAATCTGCTGGAGGTGTCAAATTAGATGAGCCTGCCATTGACCTTGCGGTAGCTATCGCAG
TAGCTTCAAGCTACAAAGAACTATCGACAGACGCGCGTGAATGCTTTATTGGCGAAATAGGATTGACTGGTGAGATCAGG
AGGGTCACTCGCATTGAGCAACGAATCAATGAAGCAGCAAAGCTCGGTTTTAAAAAAGTTTACGCTCCTAAAAACTCAAT
ATCGGGAATAGAAATCCCTGAAAATATCGAAGTAGTTGGTGTGACAACACTAATGGAATGCATGAAATTAGTCTTCTCCT
GA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(70-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A387BIA3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

79.029

100

0.79
  radA Streptococcus pneumoniae D39

79.029

100

0.79
  radA Streptococcus pneumoniae R6

79.029

100

0.79
  radA Streptococcus pneumoniae TIGR4

79.029

100

0.79
  radA Streptococcus mitis NCTC 12261

79.029

100

0.79
  radA Streptococcus mitis SK321

79.029

100

0.79
  radA Bacillus subtilis subsp. subtilis str. 168

61.589

100

0.616

Multiple sequence alignment