Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   E7Z57_RS01705 Genome accession   NZ_CP039339
Coordinates   327341..328966 (+) Length   541 a.a.
NCBI ID   WP_138928320.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain UW386     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 322341..333966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E7Z57_RS01670 (E7Z57_01670) - 323323..323901 (+) 579 WP_254706839.1 hypothetical protein -
  E7Z57_RS01675 (E7Z57_01675) - 323903..324400 (+) 498 WP_138928316.1 hypothetical protein -
  E7Z57_RS01680 (E7Z57_01680) - 324397..324873 (+) 477 WP_138928317.1 type II secretion system protein -
  E7Z57_RS01685 (E7Z57_01685) - 324867..325253 (+) 387 WP_138928318.1 type IV pilin protein -
  E7Z57_RS01690 (E7Z57_01690) - 325240..325704 (+) 465 WP_064051930.1 type II secretion system protein -
  E7Z57_RS01695 (E7Z57_01695) gspG 325708..326142 (+) 435 WP_064051931.1 type II secretion system major pseudopilin GspG -
  E7Z57_RS01700 (E7Z57_01700) - 326146..327339 (+) 1194 WP_138928319.1 type II secretion system F family protein -
  E7Z57_RS01705 (E7Z57_01705) pilF 327341..328966 (+) 1626 WP_138928320.1 GspE/PulE family protein Machinery gene
  E7Z57_RS01710 (E7Z57_01710) - 328959..329675 (+) 717 WP_138928321.1 hypothetical protein -
  E7Z57_RS01715 (E7Z57_01715) - 329665..330159 (+) 495 WP_138928322.1 hypothetical protein -
  E7Z57_RS01725 (E7Z57_01725) - 331119..332477 (+) 1359 WP_138928324.1 esterase/lipase family protein -
  E7Z57_RS01730 (E7Z57_01730) - 333300..333896 (-) 597 WP_138928325.1 plasmid pRiA4b ORF-3 family protein -

Sequence

Protein


Download         Length: 541 a.a.        Molecular weight: 58962.57 Da        Isoelectric Point: 6.0039

>NTDB_id=317307 E7Z57_RS01705 WP_138928320.1 327341..328966(+) (pilF) [Ralstonia pseudosolanacearum strain UW386]
MSELAPDWTTFGSALRAARLARGNGSLAMALAEQTGMPAQALVSAAAGRFGFHLLEAEGDLAPLVTLDPISLKDCRQHRL
LPLRVADDWVIAMDDPWDEALVRWVGEKLGQAKLAWMDGNHSLAILERALSTASASALLAQRGAEKTIQDQDVGRVVSHD
SIESTESAVVRFLDAVLLDAWRAGASDIHVETSRSGLHIKLRLDGVLVPGPAFTGARSPSEVLNRIKVLAQLDIGETRLP
QDGRFRVRLEGRDVDMRVSIMPNVFGEDAVIRLLDKAQLRGASETLTLEALGFDTAATATIRQLTKEPHGMLLVTGPTGS
GKTTTLYAALSEINTGRDKIITIEDPVEYELSGILQIPVNEKKGLTFARGLRSILRHDPDQILVGEIRDSETAEIAVQAA
LTGHLVFTSVHANSALDVVGRFLHMGVDPYNFMSALIGIVSQRLLRLKCPECAPASPDPGSRSAGCPACRYTGYRGRTVV
WEIVPVNDIIRELVIRREPLRLVREQSRQMGVVSLRDQAVRMVERGLTTFEEIDRVVSPNE

Nucleotide


Download         Length: 1626 bp        

>NTDB_id=317307 E7Z57_RS01705 WP_138928320.1 327341..328966(+) (pilF) [Ralstonia pseudosolanacearum strain UW386]
ATGTCCGAACTGGCGCCCGATTGGACCACCTTCGGCAGCGCACTGCGTGCCGCCCGCCTGGCGCGCGGCAACGGCAGCCT
GGCCATGGCGCTCGCCGAGCAGACCGGCATGCCGGCGCAGGCGCTGGTCTCGGCGGCGGCCGGCCGCTTCGGCTTCCATT
TGCTGGAGGCGGAAGGTGACCTCGCCCCGCTGGTCACGCTCGACCCGATCTCGCTCAAGGACTGCCGCCAGCACAGGCTC
CTGCCGCTGCGCGTGGCCGACGATTGGGTCATCGCCATGGACGACCCGTGGGACGAGGCGCTGGTGCGCTGGGTCGGCGA
GAAGCTCGGCCAAGCCAAGCTCGCCTGGATGGACGGCAATCACAGCCTTGCCATCCTCGAACGCGCCCTGAGCACGGCTT
CGGCCTCGGCCCTGCTCGCCCAGCGCGGCGCGGAAAAGACAATCCAGGACCAGGACGTCGGCCGCGTCGTTTCACACGAC
TCCATCGAATCGACGGAAAGCGCCGTCGTGCGCTTCCTGGACGCGGTGCTGCTCGATGCATGGCGGGCCGGCGCCAGCGA
TATCCACGTCGAGACCTCCCGCTCCGGTCTGCACATCAAGCTGCGGCTGGACGGCGTGCTGGTGCCCGGCCCCGCGTTCA
CGGGGGCGCGCTCGCCGAGCGAAGTGCTGAACCGGATCAAGGTGCTGGCCCAGTTGGACATCGGCGAGACCCGGCTGCCG
CAGGACGGCCGCTTCCGGGTCCGCCTGGAAGGGCGCGATGTCGACATGCGCGTCTCCATCATGCCCAACGTGTTCGGCGA
GGATGCCGTCATCCGCTTGCTGGACAAGGCGCAGCTGCGCGGGGCCTCGGAGACGCTCACGCTTGAGGCGCTGGGGTTCG
ACACCGCCGCCACCGCCACCATCCGTCAGTTGACCAAGGAGCCGCACGGCATGCTGCTGGTGACCGGCCCCACCGGCAGT
GGCAAGACCACCACGCTGTATGCGGCGCTGTCTGAAATCAACACCGGGCGCGACAAGATCATCACGATCGAAGACCCGGT
CGAATACGAGCTGTCGGGCATCCTGCAGATTCCCGTCAACGAGAAGAAGGGCCTGACCTTCGCGCGCGGCCTGCGCTCGA
TCCTGCGCCATGACCCGGACCAGATCCTGGTGGGCGAAATCCGCGATTCCGAGACGGCCGAGATCGCCGTGCAGGCCGCG
CTGACCGGGCACCTGGTGTTCACCTCCGTGCATGCGAACTCGGCGCTCGATGTGGTCGGCCGCTTCCTGCACATGGGCGT
CGACCCCTACAACTTCATGTCGGCACTGATCGGCATCGTGTCGCAGCGGCTGCTGCGCCTGAAATGCCCGGAATGCGCGC
CCGCCTCGCCCGACCCGGGCAGCCGGTCGGCCGGCTGTCCGGCCTGCCGGTACACGGGGTATCGGGGGCGTACCGTCGTC
TGGGAAATCGTTCCCGTCAACGACATCATCCGCGAGCTCGTGATCCGGCGCGAGCCGCTGCGGCTGGTCCGCGAGCAGAG
CCGCCAGATGGGCGTGGTGTCGCTGCGCGACCAGGCGGTGCGCATGGTGGAGCGCGGCCTGACCACGTTTGAGGAAATCG
ACCGCGTGGTGTCGCCCAATGAATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(176-446)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(27-111)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

36.531

100

0.366