Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   D7J84_RS19120 Genome accession   NZ_CP032608
Coordinates   3655731..3656165 (+) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus thuringiensis strain QZL38     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3650731..3661165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D7J84_RS19100 (D7J84_19150) - 3651635..3652798 (-) 1164 WP_000434579.1 aminotransferase A -
  D7J84_RS19105 (D7J84_19155) - 3652921..3653121 (-) 201 WP_000929280.1 hypothetical protein -
  D7J84_RS19110 (D7J84_19160) kinB 3653432..3654706 (+) 1275 WP_000420360.1 sporulation sensor histidine kinase KinB -
  D7J84_RS19115 (D7J84_19165) - 3654745..3655494 (-) 750 WP_000388466.1 DUF3967 domain-containing protein -
  D7J84_RS19120 (D7J84_19170) nucA/comI 3655731..3656165 (+) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  D7J84_RS19125 (D7J84_19175) comJ 3656202..3656591 (+) 390 WP_000424030.1 competence protein ComJ -
  D7J84_RS19130 (D7J84_19180) metE 3656630..3658918 (-) 2289 WP_061884528.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  D7J84_RS19135 (D7J84_19185) - 3659429..3659986 (-) 558 WP_000062058.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=317027 D7J84_RS19120 WP_000811494.1 3655731..3656165(+) (nucA/comI) [Bacillus thuringiensis strain QZL38]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=317027 D7J84_RS19120 WP_000811494.1 3655731..3656165(+) (nucA/comI) [Bacillus thuringiensis strain QZL38]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment