Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   D7J84_RS11075 Genome accession   NZ_CP032608
Coordinates   2161276..2162652 (-) Length   458 a.a.
NCBI ID   WP_001085202.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain QZL38     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2156276..2167652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D7J84_RS11055 (D7J84_11085) ispF 2157639..2158115 (-) 477 WP_000488388.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  D7J84_RS11060 (D7J84_11090) ispD 2158232..2158912 (-) 681 WP_000288285.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  D7J84_RS11065 (D7J84_11095) - 2158929..2160038 (-) 1110 WP_000919677.1 PIN/TRAM domain-containing protein -
  D7J84_RS11070 (D7J84_11100) disA 2160199..2161272 (-) 1074 WP_000392158.1 DNA integrity scanning diadenylate cyclase DisA -
  D7J84_RS11075 (D7J84_11105) radA 2161276..2162652 (-) 1377 WP_001085202.1 DNA repair protein RadA Machinery gene
  D7J84_RS11080 (D7J84_11110) clpC 2162748..2165183 (-) 2436 WP_000971184.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  D7J84_RS11085 (D7J84_11115) - 2165206..2166270 (-) 1065 WP_000050842.1 protein arginine kinase -
  D7J84_RS11090 (D7J84_11120) - 2166275..2166823 (-) 549 WP_061883701.1 UvrB/UvrC motif-containing protein -
  D7J84_RS11095 (D7J84_11125) ctsR 2166994..2167455 (-) 462 WP_001244565.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49844.48 Da        Isoelectric Point: 7.1422

>NTDB_id=317003 D7J84_RS11075 WP_001085202.1 2161276..2162652(-) (radA) [Bacillus thuringiensis strain QZL38]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNSLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADASYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPEGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=317003 D7J84_RS11075 WP_001085202.1 2161276..2162652(-) (radA) [Bacillus thuringiensis strain QZL38]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGTTATCAATCACCAAAATATATGGGTAAATGTCCAGGGTG
TGGTCAATGGAATTCGCTTGTTGAAGAGATGGAACCAGTTGTATCATCCAGACGCCTAAATTATGCAAATGCAATTCAAA
CAGAAGTAACAAAACCAAGACGTCTTACAGAAGTAGAAACAAAGTCTGAGGCACGTATTGAAACAAAATTTCAGGAGTTT
AACCGTGTACTAGGTGGCGGAATTGTAGATGGATCTTTAGTACTTATTGGTGGGGACCCTGGGATTGGAAAATCAACGTT
ATTATTACAGATTTCCTCGCAATTAGCAGATGCTTCATATGATGTATTGTATATATCAGGTGAGGAGTCAGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTACATGTAAATGGTAGTAATCTATTTGTTGTATCAGAGACAGATTTACAGCGTATTGCA
GCACATATTGAAGAGATGAATCCTGCTTTTGTTGTTATTGATTCTATTCAAACGATACATTTACCTGAGGTGACATCAGC
TCCTGGTAGTGTGGCGCAAGTACGTGAATGTACAGCAGAATTAATGAAACTTGCAAAAACAAAGGGAATCCCAATTTTCA
TCGTAGGGCATGTGACAAAAGAAGGTGCAATTGCGGGACCGCGTATGCTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGATCGACACCATACATATCGTATTTTACGAGCTGTAAAGAATCGTTTTGGTTCTACGAATGAAATGGGTATCTT
TGAAATGAAAGAACTTGGCCTTGCAGAAGTCTTAAATCCTTCTGAGATTTTCCTTGAGGAAAGACCAGTTGGAGTTGCAG
GTTCAACAGTAGTTGCCTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAGGCATTAATTTCCCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCGACAGGAATTGATCATAATCGTGTCTCGCTTATTATGGCGGTACTAGAGAAAAGAACAGG
TTTACTATTACAAAATCAAGATGCGTATTTAAAAGTAGCTGGTGGTTTGAAATTAGATGAACCGGCAATTGATTTAGCTG
TTGCCTTAAGTATAGCTTCAAGTTTTAGAGATAAATCTACGGCACCAACTGATGCGGTAATAGGAGAAGTTGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATCGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGTGCTATCATTCC
TAGAAAAAATTTAGGGGGGTGGACAATTCCAGAGGGGATTGAGGTTGTAGGTGTTTCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

AAA domain (AAA_25)

(60-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.728

100

0.749
  radA Streptococcus pneumoniae Rx1

64.018

98.908

0.633
  radA Streptococcus pneumoniae D39

64.018

98.908

0.633
  radA Streptococcus pneumoniae R6

64.018

98.908

0.633
  radA Streptococcus pneumoniae TIGR4

64.018

98.908

0.633
  radA Streptococcus mitis SK321

64.018

98.908

0.633
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631

Multiple sequence alignment