Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   D6022_RS12180 Genome accession   NZ_CP032538
Coordinates   2328463..2329092 (+) Length   209 a.a.
NCBI ID   WP_003183693.1    Uniprot ID   Q8VQ77
Organism   Bacillus licheniformis strain MT-B06     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2323463..2334092
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D6022_RS12145 aroE 2324105..2324941 (+) 837 WP_003183706.1 shikimate dehydrogenase -
  D6022_RS12150 yhbY 2324935..2325225 (+) 291 WP_003183704.1 ribosome assembly RNA-binding protein YhbY -
  D6022_RS12155 - 2325244..2325813 (+) 570 WP_003183702.1 nicotinate-nucleotide adenylyltransferase -
  D6022_RS12160 yqeK 2325803..2326366 (+) 564 WP_003183701.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  D6022_RS12165 rsfS 2326380..2326736 (+) 357 WP_003183699.1 ribosome silencing factor -
  D6022_RS12170 - 2326733..2327488 (+) 756 WP_003183697.1 class I SAM-dependent methyltransferase -
  D6022_RS12175 comER 2327558..2328379 (-) 822 WP_017474327.1 late competence protein ComER -
  D6022_RS12180 comEA 2328463..2329092 (+) 630 WP_003183693.1 helix-hairpin-helix domain-containing protein Machinery gene
  D6022_RS12185 - 2329161..2329730 (+) 570 WP_003183690.1 ComE operon protein 2 -
  D6022_RS12190 - 2329734..2332121 (+) 2388 WP_009327865.1 DNA internalization-related competence protein ComEC/Rec2 -
  D6022_RS12195 - 2332146..2332277 (-) 132 WP_003183686.1 YqzM family protein -
  D6022_RS12200 holA 2332507..2333550 (+) 1044 WP_025804972.1 DNA polymerase III subunit delta -
  D6022_RS12205 rpsT 2333612..2333878 (-) 267 WP_003183682.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22468.42 Da        Isoelectric Point: 5.1890

>NTDB_id=316838 D6022_RS12180 WP_003183693.1 2328463..2329092(+) (comEA) [Bacillus licheniformis strain MT-B06]
MTDWLKQYKWHAAGGVALVLIISAAFMLLSGKRETSSGLSIPEEASAQTYDKKEEVKREKSAGKEAVIIDLKGAVKNPGV
YQMKEGDRVHDVLKKAGGTEKKADQKQINLAAVLQDGMVVYIPFEGEEAADSFSKAGSRADGASVDIVNINTASSEELQA
IPGIGPSKAEAVIEYREENGPFHTVEDITNVSGIGEKSFERIKSAITVK

Nucleotide


Download         Length: 630 bp        

>NTDB_id=316838 D6022_RS12180 WP_003183693.1 2328463..2329092(+) (comEA) [Bacillus licheniformis strain MT-B06]
TTGACAGATTGGCTAAAACAATATAAATGGCATGCTGCGGGAGGCGTTGCACTCGTCTTGATCATCAGCGCTGCCTTCAT
GCTGTTAAGCGGAAAGCGTGAAACTTCATCCGGCCTCTCCATTCCCGAGGAGGCTTCTGCACAGACGTATGACAAGAAGG
AGGAGGTGAAACGGGAAAAAAGCGCGGGAAAAGAGGCGGTTATCATCGATTTGAAAGGCGCTGTGAAAAATCCGGGCGTC
TATCAAATGAAAGAGGGAGACAGGGTCCACGATGTATTGAAAAAAGCAGGCGGCACCGAGAAAAAAGCGGATCAAAAGCA
AATTAACCTTGCAGCCGTTTTGCAGGACGGTATGGTGGTTTACATTCCATTTGAGGGGGAAGAGGCTGCTGATTCTTTCT
CGAAAGCGGGTTCAAGGGCAGATGGCGCTTCCGTCGATATCGTCAATATCAATACGGCTTCCTCTGAGGAGCTTCAGGCG
ATTCCCGGCATCGGCCCTTCAAAAGCGGAAGCGGTTATCGAATACCGCGAGGAGAACGGACCGTTTCACACGGTAGAAGA
CATAACAAACGTTTCGGGAATTGGAGAAAAGTCTTTTGAAAGAATAAAATCTGCAATCACGGTAAAGTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(147-207)

SLBB domain (SLBB)

(69-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VQ77

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

51.402

100

0.526

Multiple sequence alignment