Detailed information NTG0316741

Bioinformatically predicted

Overview

• Name: sepM
• Type: Regulator
• Locus tag: D6869_RS12140
• Genome accession: NZ_CP032526
• Coordinates: 2394650..2395681 (-)
• Length: 343 a.a.
• NCBI ID: WP_011836077.1
• Uniprot ID: A0A084AC41
• Organism: Lactococcus cremoris strain 4B0
• Function: processing of CSP (predicted from homology) Competence regulation

Genomic Context

Location: 2389650..2400681

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D6869_RS13115	-	2389759..2390604 (-)	846	WP_242700777.1	radical SAM protein	-
D6869_RS12120 (D6869_12275)	-	2390788..2391645 (-)	858	WP_011836073.1	Rgg/GadR/MutR family transcriptional regulator	-
D6869_RS12125 (D6869_12280)	-	2391697..2392833 (-)	1137	WP_011836074.1	MFS transporter	-
D6869_RS12130 (D6869_12285)	kwcM	2392830..2394149 (-)	1320	WP_011836075.1	KxxxW cyclic peptide radical SAM maturase	-
D6869_RS12135 (D6869_12290)	-	2394217..2394330 (-)	114	WP_011836076.1	KxxxW-cyclized peptide pheromone	-
D6869_RS12140 (D6869_12295)	sepM	2394650..2395681 (-)	1032	WP_011836077.1	SepM family pheromone-processing serine protease	Regulator
D6869_RS12145 (D6869_12300)	coaD	2395665..2396165 (-)	501	WP_011836078.1	pantetheine-phosphate adenylyltransferase	-
D6869_RS12150 (D6869_12305)	rsmD	2396213..2396752 (-)	540	WP_207677836.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
D6869_RS12155 (D6869_12310)	-	2396925..2398142 (-)	1218	WP_011836080.1	MFS transporter	-
D6869_RS12160 (D6869_12315)	lmrP	2398227..2399453 (-)	1227	WP_011836081.1	multidrug efflux MFS transporter LmrP	-
D6869_RS12165 (D6869_12320)	-	2399688..2400131 (+)	444	WP_041931231.1	sigma-70 family RNA polymerase sigma factor	-

Sequence

Protein

• Length: 343 a.a.
• Molecular weight: 37403.02 Da
• Isoelectric Point: 9.9119

>NTDB_id=316741 D6869_RS12140 WP_011836077.1 2394650..2395681(-) (sepM) [Lactococcus cremoris strain 4B0]
MNKKNKKISPKLKWGISIGLIIVALLVLVYPTNYYVEMPGTTEPLGKMVKVEGKKDEHKGDFFLTTVQIARANLATMIYS
HFNSFTSIYSEQEMTGGLNDAQFNRVNQFYMETAQNTAVYQAFKLANKPYELKYEGVYVLDIAKNSTFKNKLELSDTITA
VNGEEFKSSADMIAYVSKQKVGDSVTIEYTRIDGSKHKSTGKYIKISNGKTGIGIGLVDHTEVVTDPKVTVNAGSIGGPS
AGMMFTLEIYSQLTGKNLRGGREIAGTGTIEHDGSIGQIGGVDKKVATASKEGAKVFLVPDSGTKKESSNNYLGAKAAAK
KLKTKMKIVPVKTIQDALTYLEK

Nucleotide

• Length: 1032 bp

>NTDB_id=316741 D6869_RS12140 WP_011836077.1 2394650..2395681(-) (sepM) [Lactococcus cremoris strain 4B0]
ATGAACAAAAAAAATAAAAAAATCAGTCCCAAATTAAAATGGGGCATCTCTATTGGCCTGATTATTGTCGCTCTCCTTGT
TTTAGTGTATCCAACAAATTACTATGTGGAAATGCCTGGAACAACCGAGCCATTAGGGAAAATGGTCAAAGTTGAAGGAA
AAAAAGATGAGCACAAAGGTGACTTTTTCCTTACTACCGTTCAAATTGCGCGTGCTAATCTTGCCACAATGATTTACAGT
CATTTTAATAGTTTTACAAGCATTTACAGTGAACAAGAAATGACCGGTGGTCTTAATGATGCGCAGTTCAATCGTGTCAA
CCAGTTTTACATGGAAACTGCACAAAATACAGCCGTTTATCAGGCCTTCAAGTTGGCAAATAAACCCTATGAACTGAAAT
ATGAAGGGGTTTATGTCCTTGATATCGCTAAAAATTCGACTTTTAAAAATAAATTGGAACTTTCGGACACGATTACAGCA
GTCAATGGGGAAGAATTTAAATCAAGTGCAGATATGATTGCTTACGTTTCGAAGCAAAAAGTCGGCGATTCAGTTACCAT
TGAATATACACGGATTGATGGCAGTAAACACAAGTCTACTGGAAAATATATCAAAATCTCTAATGGAAAAACCGGAATCG
GAATTGGACTAGTTGACCATACTGAAGTAGTCACAGACCCAAAAGTTACAGTCAATGCAGGTTCAATCGGTGGACCATCT
GCCGGAATGATGTTTACACTAGAGATTTACAGTCAGTTGACAGGTAAAAATTTACGTGGTGGTCGCGAAATAGCAGGAAC
TGGAACGATTGAACATGATGGAAGCATTGGCCAAATCGGTGGGGTTGATAAAAAAGTAGCTACTGCTAGCAAAGAAGGCG
CAAAAGTTTTCTTAGTTCCTGACTCAGGCACCAAAAAAGAAAGCAGCAATAACTATCTTGGAGCCAAAGCAGCCGCTAAA
AAATTAAAAACGAAGATGAAAATTGTTCCCGTCAAGACTATTCAGGACGCTTTAACTTATTTAGAAAAATAA

Domains

Predicted by InterproScan.

• Lon protease (S16) C-terminal proteolytic domain (Lon_C) (229-339)

• PDZ domain (PDZ_2) (135-193)

3D structure

• AlphaFold DB: A0A084AC41

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
sepM	Streptococcus mutans UA159	48.406	100	0.487