Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E5Q09_RS05910 Genome accession   NZ_CP038991
Coordinates   1174428..1175501 (-) Length   357 a.a.
NCBI ID   WP_010887806.1    Uniprot ID   Q9RV67
Organism   Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 strain ATCC 13939 substr. R6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1169428..1180501
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5Q09_RS05880 - 1169693..1170925 (-) 1233 WP_027479609.1 glycerate kinase type-2 family protein -
  E5Q09_RS05885 uraD 1171190..1171756 (+) 567 WP_010887801.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
  E5Q09_RS05890 - 1171893..1172426 (-) 534 WP_034349584.1 MarR family winged helix-turn-helix transcriptional regulator -
  E5Q09_RS05895 pucL 1172540..1173436 (+) 897 WP_010887803.1 factor-independent urate hydroxylase -
  E5Q09_RS05900 uraH 1173433..1173792 (+) 360 WP_010887804.1 hydroxyisourate hydrolase -
  E5Q09_RS05905 - 1173903..1174379 (-) 477 WP_010887805.1 transcription elongation factor GreA -
  E5Q09_RS05910 pilT 1174428..1175501 (-) 1074 WP_010887806.1 type IV pilus twitching motility protein PilT Machinery gene
  E5Q09_RS05915 panC 1175498..1176343 (-) 846 WP_010887807.1 pantoate--beta-alanine ligase -
  E5Q09_RS05920 - 1176396..1176767 (-) 372 WP_010887808.1 phage holin family protein -
  E5Q09_RS05925 - 1176909..1177493 (+) 585 WP_010887809.1 hypothetical protein -
  E5Q09_RS05930 - 1177526..1178128 (+) 603 WP_010887810.1 DedA family protein -
  E5Q09_RS05935 nudC 1178097..1178939 (-) 843 WP_010887811.1 NAD(+) diphosphatase -
  E5Q09_RS05940 - 1178936..1180075 (-) 1140 WP_010887812.1 DUF2201 family putative metallopeptidase -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 39412.17 Da        Isoelectric Point: 6.2347

>NTDB_id=316660 E5Q09_RS05910 WP_010887806.1 1174428..1175501(-) (pilT) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 strain ATCC 13939 substr. R6]
MTLDDLLRELAGRRASDIHLQAGSPPLGRVDGRLLPFGTQPLTPEHTQALARAVLTPEQFEEFEYQQELDVAYSVPGLGR
FRCNVFRQRGAVGLVLRVVGETIPGFEALGLPREVMEQFAALPRGLVLITGPTGSGKTTTVASLIDYLNRHFAYHILTIE
DPIEVLHRNNRSLVVQREVGRDTRSFAAALRHALRQDPDVIVIGEIRDRETVEAALLAAQTGHLVISTLHTQDAVRTVGR
IAEFFAPHERDQVRLQLAECLGGILSQRLLPRADGVGRALATEVLVSTPLVQEYVKDEARTPLLKDVLLEDNLRGMHTFD
EHLAALYRAELITLDAALAAATSPHELRLLLTQTGRL

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=316660 E5Q09_RS05910 WP_010887806.1 1174428..1175501(-) (pilT) [Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539 strain ATCC 13939 substr. R6]
ATGACGCTGGACGACCTGCTGCGCGAACTCGCGGGCCGCCGCGCCTCCGACATCCACCTGCAAGCGGGCAGTCCGCCGCT
GGGCCGGGTGGATGGCCGCTTGCTGCCCTTTGGCACGCAACCACTGACGCCGGAGCATACCCAGGCGCTCGCACGGGCGG
TGCTGACCCCTGAGCAGTTTGAGGAATTCGAGTACCAGCAGGAACTGGACGTGGCCTACAGCGTGCCGGGTCTGGGCCGC
TTCCGCTGCAACGTGTTCCGGCAGCGCGGTGCGGTGGGCCTGGTGCTGCGGGTGGTGGGCGAAACCATTCCGGGCTTCGA
GGCACTCGGACTACCGCGCGAAGTCATGGAGCAGTTCGCGGCGCTGCCCCGTGGCCTGGTGCTGATTACCGGGCCGACCG
GCAGTGGCAAGACCACCACCGTCGCCAGCCTCATCGACTACCTCAACCGGCACTTCGCCTATCACATTCTGACCATCGAA
GACCCCATCGAGGTGCTGCACCGCAACAACCGCAGTCTGGTGGTACAGCGCGAGGTGGGGCGCGACACTCGCAGTTTCGC
GGCGGCGCTGCGGCACGCGCTGCGTCAAGACCCCGACGTGATCGTGATCGGCGAGATTCGGGACCGCGAAACGGTGGAGG
CCGCGCTGCTGGCGGCGCAGACCGGGCATCTGGTCATCAGCACGCTGCACACCCAGGACGCGGTGCGGACGGTAGGGCGC
ATCGCCGAATTTTTCGCCCCCCACGAGCGCGATCAGGTGCGGCTGCAACTCGCCGAGTGCCTGGGCGGCATCCTCAGCCA
GCGGCTGCTGCCCCGCGCCGACGGGGTGGGCCGGGCACTGGCGACCGAGGTGCTCGTCAGCACGCCCCTGGTGCAGGAAT
ATGTCAAGGACGAGGCGCGCACGCCGCTGCTCAAGGACGTGCTGCTCGAAGACAATCTGCGGGGGATGCACACCTTCGAC
GAGCACCTGGCCGCGCTCTACCGTGCCGAGCTGATCACCCTCGACGCGGCGCTCGCGGCGGCCACCAGCCCGCACGAACT
GCGGCTGCTGCTGACCCAGACCGGGCGACTCTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9RV67

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.667

96.639

0.451
  pilT Pseudomonas aeruginosa PAK

42.075

97.199

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.595

98.319

0.409
  pilT Pseudomonas stutzeri DSM 10701

41.787

97.199

0.406
  pilT Acinetobacter baylyi ADP1

41.499

97.199

0.403
  pilU Vibrio cholerae strain A1552

41.026

98.319

0.403
  pilT Neisseria meningitidis 8013

41.246

94.398

0.389
  pilT Neisseria gonorrhoeae MS11

41.246

94.398

0.389
  pilT Legionella pneumophila strain Lp02

40.762

95.518

0.389
  pilT Legionella pneumophila strain ERS1305867

40.762

95.518

0.389
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.025

91.317

0.384
  pilT Vibrio cholerae strain A1552

42.025

91.317

0.384
  pilU Acinetobacter baylyi ADP1

39.143

98.039

0.384
  pilT Acinetobacter baumannii D1279779

39.481

97.199

0.384
  pilT Acinetobacter baumannii strain A118

39.481

97.199

0.384
  pilT Acinetobacter nosocomialis M2

39.481

97.199

0.384

Multiple sequence alignment