Detailed information NTG0316553

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: D5H27_RS15655
• Genome accession: NZ_CP032506
• Coordinates: 3148753..3149898 (+)
• Length: 381 a.a.
• NCBI ID: WP_074534220.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain JT3-1
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3143753..3154898

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D5H27_RS15630 (D5H27_15430)	-	3144650..3145078 (-)	429	WP_032869448.1	DUF1934 domain-containing protein	-
D5H27_RS15635 (D5H27_15435)	-	3145232..3145744 (+)	513	Protein_3032	bacteriocin biosynthesis protein AlbG	-
D5H27_RS15640 (D5H27_15440)	-	3145751..3147127 (-)	1377	WP_032875890.1	YncE family protein	-
D5H27_RS15645 (D5H27_15445)	-	3147167..3148522 (-)	1356	WP_032875887.1	YncE family protein	-
D5H27_RS15655 (D5H27_15450)	rapF	3148753..3149898 (+)	1146	WP_074534220.1	Rap family tetratricopeptide repeat protein	Regulator
D5H27_RS15660 (D5H27_15455)	-	3149882..3150001 (+)	120	WP_017418707.1	PhrC/PhrF family phosphatase-inhibitory pheromone	-
D5H27_RS15665 (D5H27_15460)	speB	3150124..3150996 (-)	873	WP_032875884.1	agmatinase	-
D5H27_RS15670 (D5H27_15465)	speE	3151055..3151885 (-)	831	WP_003151052.1	spermidine synthase	-
D5H27_RS15675 (D5H27_15470)	-	3152085..3154157 (+)	2073	WP_032875881.1	transglycosylase domain-containing protein	-
D5H27_RS15680 (D5H27_15475)	-	3154182..3154615 (-)	434	Protein_3040	hypothetical protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45737.21 Da
• Isoelectric Point: 5.0614

>NTDB_id=316553 D5H27_RS15655 WP_074534220.1 3148753..3149898(+) (rapF) [Bacillus velezensis strain JT3-1]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAESKLTYVKDHIEKAEFYFKMSEVYYYMKQTYVS
MDYARQAYLIYKDAEKYNIRLTQCHSLFGANYLDVKQYDHAISHFQKAYTMAELEQQPQLMGRTLFNIALCHNNQNNKVE
AIPHLKEAIAVFEKAQMSHSLPQPYFLLTQIYFKLKNIDNAYDYHKKGISICKEIDDIIYEEKFKFLEYLYMPDPRDTLI
NDCLHFLENKLMYADIEELALDVAKYYYTQEEYKKASVYFLKVEEARQQIEGGVNLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=316553 D5H27_RS15655 WP_074534220.1 3148753..3149898(+) (rapF) [Bacillus velezensis strain JT3-1]
GTGGCGAGTGTTTTATCATCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAACTTGCAGGTCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGTCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAAAGCAAACTGACGTACG
TTAAAGATCATATAGAAAAAGCTGAATTTTACTTTAAAATGTCAGAAGTCTATTATTATATGAAACAAACTTATGTATCG
ATGGATTATGCGCGGCAGGCTTATTTAATTTATAAAGATGCAGAAAAATATAATATTAGATTAACCCAGTGCCATTCTTT
ATTTGGGGCTAATTATCTTGATGTAAAGCAATACGATCACGCGATTTCCCACTTTCAAAAGGCATATACGATGGCTGAAT
TGGAGCAGCAGCCGCAATTAATGGGGAGGACACTTTTTAACATCGCTTTATGCCATAATAATCAAAACAATAAAGTCGAG
GCTATTCCCCATTTAAAAGAAGCCATAGCTGTTTTTGAAAAAGCTCAAATGAGTCATTCACTTCCGCAGCCCTACTTCTT
ACTTACACAGATTTATTTTAAGTTAAAAAATATAGATAACGCGTACGATTATCATAAAAAAGGTATCTCAATATGTAAGG
AAATAGATGATATAATTTACGAGGAAAAATTTAAATTTTTAGAATACTTGTATATGCCGGATCCAAGAGATACATTAATA
AACGATTGTCTGCATTTTCTTGAAAATAAATTGATGTATGCTGATATTGAGGAATTAGCCCTTGATGTAGCTAAATATTA
TTACACACAAGAAGAATATAAAAAAGCTTCTGTCTACTTTTTAAAGGTTGAAGAAGCCAGACAGCAGATCGAAGGAGGAG
TGAATTTATATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (5-65)

• Tetratricopeptide repeat (TPR_12) (188-254)

• Tetratricopeptide repeat (TPR_8) (265-293)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	70.079	100	0.701
rapC	Bacillus subtilis subsp. subtilis str. 168	54.211	99.738	0.541