Detailed information NTG0316530

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: D5H27_RS10015
• Genome accession: NZ_CP032506
• Coordinates: 2082600..2084951 (-)
• Length: 783 a.a.
• NCBI ID: WP_032873958.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain JT3-1
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2077600..2089951

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D5H27_RS09980 (D5H27_09860)	-	2077966..2078304 (-)	339	WP_007408270.1	YqxA family protein	-
D5H27_RS09985 (D5H27_09865)	-	2078321..2079514 (-)	1194	WP_032873961.1	stage II sporulation protein P	-
D5H27_RS09990 (D5H27_09870)	gpr	2079582..2080688 (-)	1107	WP_007408268.1	GPR endopeptidase	-
D5H27_RS09995 (D5H27_09875)	rpsT	2080891..2081157 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
D5H27_RS10000 (D5H27_09880)	holA	2081174..2082215 (-)	1042	Protein_1947	DNA polymerase III subunit delta	-
D5H27_RS10005 (D5H27_09885)	-	2082255..2082406 (-)	152	Protein_1948	hypothetical protein	-
D5H27_RS10010 (D5H27_09890)	-	2082447..2082581 (+)	135	WP_003152870.1	YqzM family protein	-
D5H27_RS10015 (D5H27_09895)	comEC	2082600..2084951 (-)	2352	WP_032873958.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
D5H27_RS10020 (D5H27_09900)	-	2084952..2085521 (-)	570	WP_003152868.1	ComE operon protein 2	-
D5H27_RS10025 (D5H27_09905)	comEA	2085588..2086202 (-)	615	WP_032873955.1	helix-hairpin-helix domain-containing protein	Machinery gene
D5H27_RS10030 (D5H27_09910)	comER	2086261..2087082 (+)	822	WP_012118027.1	late competence protein ComER	-
D5H27_RS10035 (D5H27_09915)	-	2087151..2087888 (-)	738	WP_032873951.1	class I SAM-dependent methyltransferase	-
D5H27_RS10040 (D5H27_09920)	rsfS	2087885..2088241 (-)	357	WP_007408260.1	ribosome silencing factor	-
D5H27_RS10045 (D5H27_09925)	yqeK	2088245..2088820 (-)	576	WP_032873949.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
D5H27_RS10050 (D5H27_09930)	-	2088810..2089379 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
D5H27_RS10055 (D5H27_09935)	yhbY	2089390..2089680 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-

Sequence

Protein

• Length: 783 a.a.
• Molecular weight: 86681.60 Da
• Isoelectric Point: 9.1106

>NTDB_id=316530 D5H27_RS10015 WP_032873958.1 2082600..2084951(-) (comEC) [Bacillus velezensis strain JT3-1]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIIVKTKHHAPVIVCLVSFCVYFFLYTVCDAANVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATVPGGFDYKEYLHSQQI
HWLFSVTSIQQCEKSKQPLFKLLNIRKNLISIIRNHVPESSAGIVEALTLGEKFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGILLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLMFNP
YFLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASFIAELSSLPFLLYHFQQISLVSFLMNMVLVPFYTLFVIPV
SVIGFLLLLLSRQMGECLFDMFDLVMKPVHDFITYAASVDLFTMIVSKPDFVSLLLLAVSVFTLFAALEKGGFLKLRKSA
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVISYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGRSKNDSSLVLWTVLGGVSWLLTGDLESDGETEVLKTYPKLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKVYSVNVLRTDVSGTIQYRFKKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide

• Length: 2352 bp

>NTDB_id=316530 D5H27_RS10015 WP_032873958.1 2082600..2084951(-) (comEC) [Bacillus velezensis strain JT3-1]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATTGTAAAAACAAAGCACCATGCTCCGGTTATCGTCTGCCTCGTTT
CTTTTTGTGTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGGTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAATATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGTTCTCTTGAGGAAAAAAGACTTATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGGTTCCCGGAGGTTTTGATTATAAGGAATATCTTCACTCTCAGCAGATT
CACTGGTTATTTTCCGTGACTTCCATTCAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAACATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAAAT
TTTCTATAGAGGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGATTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAAGCGGGAATTTTGCTGCTGCT
GTTTTTGCCGGTGTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCCCTGTCTTATCTGCTGCTCCTGATGTTTAATCCT
TACTTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCGGGGAAAAGCAGACTTGCCGGGCTTGCGATGGCTTCATTCATTGCGGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAGATTTCACTTGTCAGTTTTCTGATGAATATGGTGTTGGTGCCATTTTATACGTTATTTGTCATTCCGGTT
TCTGTTATCGGTTTCCTTCTTCTTTTACTTTCAAGGCAGATGGGAGAATGTTTGTTTGATATGTTCGACCTTGTGATGAA
GCCTGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTCAAAGCCTGATTTTGTTTCCC
TTCTTCTGCTTGCGGTTTCAGTTTTTACGCTTTTTGCGGCTTTGGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCACTGTTTATAAGCGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTTCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCGTTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAAGATCACATCGGAGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTGGGATTCGTAAAAGAACCGAAAGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGTGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAGGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTAGGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACGGAAGTGCTGAAAACGTATCCGAAACTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCTACGAGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCAGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCATGAAGAAGTGCTGGATCGTTTGAAGGTGTACTCTGTCAATGTGCTTCGCACCGATGT
CAGCGGAACGATTCAATACAGATTTAAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

• Metallo-beta-lactamase superfamily (Lactamase_B) (508-719)

• Competence protein (Competence) (208-469)

• Domain of unknown function (DUF4131) (DUF4131) (25-171)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Bacillus subtilis subsp. subtilis str. 168	56.606	98.595	0.558