Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   D5H27_RS08280 Genome accession   NZ_CP032506
Coordinates   1723026..1723634 (+) Length   202 a.a.
NCBI ID   WP_012117837.1    Uniprot ID   A0AAP5V0D3
Organism   Bacillus velezensis strain JT3-1     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1718026..1728634
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5H27_RS08240 (D5H27_08125) - 1719046..1719552 (-) 507 WP_012117833.1 PTS glucose transporter subunit IIA -
  D5H27_RS08245 (D5H27_08130) - 1719691..1720104 (+) 414 WP_017418017.1 hypothetical protein -
  D5H27_RS08250 (D5H27_08135) - 1720134..1720601 (-) 468 WP_029973659.1 YppG family protein -
  D5H27_RS08255 (D5H27_08140) - 1720788..1720970 (+) 183 WP_003153532.1 YppF family protein -
  D5H27_RS08260 (D5H27_08145) - 1721009..1721377 (-) 369 WP_007409492.1 YppE family protein -
  D5H27_RS08265 (D5H27_08150) - 1721423..1721668 (-) 246 WP_032873277.1 DUF5446 family protein -
  D5H27_RS08270 (D5H27_08155) sspM 1721874..1721978 (+) 105 WP_003153525.1 acid-soluble spore protein SspM -
  D5H27_RS08275 (D5H27_08160) - 1722022..1722984 (-) 963 WP_032873275.1 DUF2515 domain-containing protein -
  D5H27_RS08280 (D5H27_08165) recU 1723026..1723634 (+) 609 WP_012117837.1 Holliday junction resolvase RecU Machinery gene
  D5H27_RS08285 (D5H27_08170) - 1723673..1726456 (+) 2784 WP_032873273.1 PBP1A family penicillin-binding protein -
  D5H27_RS08290 (D5H27_08175) - 1726534..1727025 (-) 492 WP_007612186.1 YpoC family protein -
  D5H27_RS08295 (D5H27_08180) nth 1727022..1727681 (-) 660 WP_007612187.1 endonuclease III -
  D5H27_RS08300 (D5H27_08185) dnaD 1727700..1728398 (-) 699 WP_003153516.1 DNA replication protein DnaD -

Sequence

Protein


Download         Length: 202 a.a.        Molecular weight: 23666.75 Da        Isoelectric Point: 9.5778

>NTDB_id=316518 D5H27_RS08280 WP_012117837.1 1723026..1723634(+) (recU) [Bacillus velezensis strain JT3-1]
MIRYPNGKTFQPNKTVSSQNSQKRSHSYSNRGMTLEDDLNETNKYYLANQIAVIHKKPTPVQIVNVHYPKRSAAVIKEAY
FKQSSTTDYNGIYKGRYIDFEAKETKNKTAFPLQNFHDHQIEHMKQVVRQDGICFVIISAFGKVYFLEADKLFVFWERKE
NNGRKSIRKDELEDASFPISLGYSPRIDYISIIEQLYFSQEQ

Nucleotide


Download         Length: 609 bp        

>NTDB_id=316518 D5H27_RS08280 WP_012117837.1 1723026..1723634(+) (recU) [Bacillus velezensis strain JT3-1]
GTGATTCGGTACCCGAATGGAAAAACTTTTCAGCCGAACAAAACGGTTTCATCCCAAAACAGCCAGAAACGGTCTCATTC
ATACAGTAATCGCGGCATGACCCTCGAAGACGATCTGAATGAAACGAATAAGTATTATCTGGCTAACCAAATTGCTGTCA
TTCACAAAAAGCCGACACCCGTCCAGATTGTAAACGTCCACTATCCGAAAAGAAGCGCCGCAGTCATTAAGGAAGCTTAT
TTTAAACAGTCTTCCACAACCGACTACAACGGCATTTATAAAGGACGCTACATTGATTTTGAGGCAAAAGAAACAAAAAA
CAAAACGGCATTTCCCCTGCAGAATTTTCATGATCATCAGATTGAACATATGAAGCAGGTGGTCAGGCAAGACGGCATTT
GTTTTGTTATTATATCCGCTTTCGGAAAGGTTTATTTCCTCGAAGCCGACAAGCTGTTTGTCTTTTGGGAGAGAAAGGAG
AACAATGGCAGAAAATCAATTCGCAAAGACGAATTAGAAGATGCTTCCTTTCCGATTTCCCTTGGATACTCACCGAGAAT
TGATTATATTAGTATTATTGAACAGCTTTATTTTTCGCAGGAACAGTAG

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

90.452

98.515

0.891

Multiple sequence alignment