Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   D5S12_RS22260 Genome accession   NZ_CP032459
Coordinates   5153408..5154775 (-) Length   455 a.a.
NCBI ID   WP_002555235.1    Uniprot ID   A0A108WX83
Organism   Pseudomonas syringae strain CAS02     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5148408..5159775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5S12_RS22240 (D5S12_23310) yjiA 5149360..5150337 (-) 978 WP_003368700.1 GTPase -
  D5S12_RS22245 (D5S12_23315) - 5150433..5150630 (-) 198 WP_003313802.1 YbdD/YjiX family protein -
  D5S12_RS22250 (D5S12_23320) - 5150658..5152733 (-) 2076 WP_003368701.1 carbon starvation CstA family protein -
  D5S12_RS22255 (D5S12_23325) - 5153019..5153393 (+) 375 WP_003368702.1 PilZ domain-containing protein -
  D5S12_RS22260 (D5S12_23330) radA 5153408..5154775 (-) 1368 WP_002555235.1 DNA repair protein RadA Machinery gene
  D5S12_RS22265 (D5S12_23335) mscL 5154972..5155418 (+) 447 WP_003368707.1 large-conductance mechanosensitive channel protein MscL -
  D5S12_RS22270 (D5S12_23340) - 5155681..5156457 (-) 777 WP_003431625.1 ferredoxin--NADP reductase -
  D5S12_RS22275 (D5S12_23345) - 5156794..5157027 (+) 234 WP_003340758.1 helix-turn-helix domain-containing protein -
  D5S12_RS22280 (D5S12_23350) - 5157158..5158282 (+) 1125 WP_003431638.1 methyltransferase -
  D5S12_RS22285 (D5S12_23355) - 5158376..5158543 (-) 168 WP_003368712.1 DUF2474 domain-containing protein -
  D5S12_RS22290 (D5S12_23360) cydB 5158565..5159572 (-) 1008 WP_003368713.1 cytochrome d ubiquinol oxidase subunit II -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48913.48 Da        Isoelectric Point: 7.1316

>NTDB_id=316035 D5S12_RS22260 WP_002555235.1 5153408..5154775(-) (radA) [Pseudomonas syringae strain CAS02]
MAKAKRLYGCTECGATFPKWAGQCSECGAWNTLVETMIESGAAAPPTGRTGWTGSQAQIRTLAEVSVEEIPRFSTNSTEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIAQRMPALYVTGEESQQQVAMRARRLGLPQDKLRVMTETCIETIIA
TAKVEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHMS
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPPGLQIIAVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=316035 D5S12_RS22260 WP_002555235.1 5153408..5154775(-) (radA) [Pseudomonas syringae strain CAS02]
ATGGCCAAGGCCAAACGCTTGTACGGCTGCACCGAGTGCGGCGCGACATTTCCCAAGTGGGCGGGCCAGTGCAGCGAATG
CGGTGCCTGGAACACACTGGTCGAAACCATGATTGAAAGCGGTGCTGCCGCGCCGCCTACCGGGCGTACCGGCTGGACCG
GTTCCCAGGCGCAGATCAGGACGCTGGCGGAGGTCAGTGTCGAAGAGATTCCGCGCTTCTCCACCAACTCCACCGAGCTG
GACAGGGTGCTCGGCGGCGGTCTGGTTGATGGTTCGGTGGTGCTGATCGGCGGTGACCCCGGCATCGGCAAGTCCACGAT
CCTCTTGCAGACGCTGTGCAACATTGCCCAGCGCATGCCCGCGTTATACGTCACTGGTGAGGAATCCCAGCAGCAGGTCG
CCATGCGTGCCCGTCGTCTGGGACTGCCGCAGGACAAGCTGCGGGTCATGACCGAAACCTGCATCGAGACCATCATTGCC
ACCGCCAAGGTCGAGAAGCCCAAGGTGATGGTGATCGATTCGATCCAGACGATTTTCACCGAACAGTTGCAGTCGGCCCC
CGGCGGCGTGTCGCAGGTTCGCGAAAGCGCTGCCTTGCTGGTGCGTTACGCCAAGCAGAGCGGCACGGCGATCTTCCTGG
TCGGTCACGTCACCAAGGAAGGCGCCTTGGCCGGTCCGCGCGTGCTTGAGCACATGGTCGACACCGTGTTGTATTTCGAG
GGCGAGTCGGACGGGCGCCTGCGCCTGCTGCGGGCGGTGAAGAACCGCTTTGGCGCGGTCAACGAGCTGGGCGTGTTCGG
CATGACCGACAAGGGCCTGAAAGAAGTCTCCAATCCGTCTGCGATCTTTCTTACCCGTGCGCAGGAAGAAGTGCCGGGCA
GTGTGGTCATGGCGACCTGGGAAGGCACGCGGCCGATGCTGGTGGAGGTGCAGGCGCTGGTGGACGACAGTCACATGTCC
AACCCGCGCCGCGTGACGCTGGGTCTGGATCAGAATCGTCTGGCCATGCTGCTGGCTGTTCTGCACCGCCACGGCGGCAT
TCCCACTCACGACCAGGACGTGTTTCTCAACGTGGTAGGTGGGGTGAAGGTGCTGGAAACCGCATCCGACCTGGCCTTGA
TGGCAGCCGTAATGTCCAGCCTGCGCAATCGTCCGCTGCCGCATGACTTGCTGGTGTTCGGTGAGGTGGGGCTGTCGGGT
GAAGTCAGGCCGGTGCCCAGTGGACAGGAGCGCCTCAAGGAGGCCGCCAAGCACGGCTTCAAACGCGCCATCGTGCCCAA
GGGCAATGCGCCTAAGGAGGCGCCGCCCGGTTTGCAGATCATTGCCGTTACGCGACTGGAACAGGCCCTGGATGCGCTGT
TCGAGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(73-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A108WX83

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.123

100

0.492
  radA Streptococcus pneumoniae Rx1

46.739

100

0.473
  radA Streptococcus pneumoniae R6

46.739

100

0.473
  radA Streptococcus pneumoniae TIGR4

46.739

100

0.473
  radA Streptococcus pneumoniae D39

46.739

100

0.473
  radA Streptococcus mitis SK321

46.93

100

0.47
  radA Streptococcus mitis NCTC 12261

46.711

100

0.468

Multiple sequence alignment