Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   D5S12_RS01955 Genome accession   NZ_CP032459
Coordinates   419739..421958 (-) Length   739 a.a.
NCBI ID   WP_003365984.1    Uniprot ID   -
Organism   Pseudomonas syringae strain CAS02     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 414739..426958
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5S12_RS01935 (D5S12_02095) hslU 415067..416404 (-) 1338 WP_024661527.1 ATP-dependent protease ATPase subunit HslU -
  D5S12_RS01940 (D5S12_02100) hslV 416471..417001 (-) 531 WP_003392345.1 ATP-dependent protease subunit HslV -
  D5S12_RS01945 (D5S12_02105) - 417208..417903 (-) 696 WP_003432151.1 SPOR domain-containing protein -
  D5S12_RS01950 (D5S12_02110) argS 417905..419641 (-) 1737 WP_054081257.1 arginine--tRNA ligase -
  D5S12_RS01955 (D5S12_02115) priA 419739..421958 (-) 2220 WP_003365984.1 primosomal protein N' Machinery gene
  D5S12_RS01960 (D5S12_02120) rpmE 422128..422349 (+) 222 WP_002551648.1 50S ribosomal protein L31 -
  D5S12_RS01965 (D5S12_02125) - 422363..423166 (+) 804 WP_074806671.1 thermonuclease family protein -
  D5S12_RS01970 (D5S12_02130) - 423349..424617 (+) 1269 WP_003365988.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81870.19 Da        Isoelectric Point: 8.5462

>NTDB_id=316012 D5S12_RS01955 WP_003365984.1 419739..421958(-) (priA) [Pseudomonas syringae strain CAS02]
MPDAILRLALPSPLRRLFDYRAPAGVSRSALQPGMRLRVPFGRREMIGILVEVVDHSEVPADKLKPAIALLDSEAPLPPA
LFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAESRQERFWHVAPGASVDDPRIARAPKQREALTTLAQHPHGVAHQLL
SKLMLNKDSLNLLLAKELVYVEVRSHAPSARHEHWLAQPELPLNTEQRAAYEAIRAGFDSFHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALVHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNQRAGGAQQPRFMRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSGCQRCDARMTVHQRSGELRCHHCGYVERVPRQC
PSCGKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFTTIQRGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLIQLTEQGYFAFAEQALSERRSAGLPPFSHL
ALLRAEAHKPGQAEAFLDQACSDAEQLVAQMALGGIELLGPVPAPMERRAGRYRAQLLVQSSARAPLHKLLATWLLALEQ
MPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=316012 D5S12_RS01955 WP_003365984.1 419739..421958(-) (priA) [Pseudomonas syringae strain CAS02]
GTGCCCGACGCCATCCTGCGCCTTGCCCTGCCCTCGCCGCTTCGCCGCCTGTTCGACTACCGCGCCCCAGCGGGCGTGTC
GCGCAGCGCCTTGCAGCCGGGCATGCGTCTGCGAGTGCCGTTCGGACGCCGGGAAATGATCGGCATTCTGGTGGAAGTGG
TCGATCACAGCGAAGTGCCCGCCGACAAGCTCAAGCCGGCAATCGCCCTGCTCGACAGCGAAGCGCCGCTGCCACCTGCG
CTGTTCAAACTGTGCCTGTGGACCTCGCAGTATTACCAGCACAGCCTGGGCGACACGCTGAGCTGGGCCTTACCGGTGTT
GCTGCGTCAGGGCGAACTGGCCGAAAGCCGCCAGGAACGCTTCTGGCACGTTGCACCGGGTGCCAGCGTCGACGATCCGC
GCATCGCCCGCGCGCCCAAGCAGCGCGAGGCCCTGACCACCCTTGCCCAGCACCCGCACGGGGTGGCGCATCAACTGCTC
AGCAAGCTGATGCTCAACAAGGACAGCCTCAACCTGTTGCTGGCCAAGGAGCTGGTGTACGTCGAGGTCCGCAGCCATGC
GCCCAGCGCACGCCATGAACACTGGCTGGCCCAGCCTGAATTGCCGCTCAACACCGAACAACGCGCCGCTTACGAAGCGA
TCCGCGCCGGGTTCGACAGCTTTCATGCGTTCCTGCTGGCCGGCGTCACCGGCAGCGGCAAGACCGAAGTCTATCTGCAA
CTGATCCGCGAGACCCTCGAGGCGGGCAAGCAGGCGCTGGTGCTGATCCCGGAAATCAACCTCGGCCCGCAGACCCTGGC
GCGCTTCGAGCAGCGCTTCAATGCGCGCATTGCGCTGGTCCACTCGGCGGTCAACGACCGCGAACGGCTGGATGCTTGGC
TGGCGGCTCGCGATGGCGAGGCGGACATCATCATCGGCACGCGTTCGGCGCTGTTCACGCCGATGAAAAACCCCGGGCTG
ATCATCATCGACGAGGAACACGACGGCTCCTATAAACAGCAGGAAGGTCTGCGCTATCACGCCCGCGATCTGGCCCTGGT
GCGGGCGCGTCAGGAAAACATTCCGATTGTCCTGGGCTCGGCCACTCCGTCCCTGGAAAGCCTGCATAACGCCTACACTG
GCCGCTACGGCCTGCTGCGCCTGAATCAGCGCGCGGGCGGCGCACAGCAGCCGCGTTTCATGCGCCTGGACGTCAAGAGC
CGACCGCTGGACAGCGGCATCTCCGGGCCCATGCAGCAGGCCATCGGTCAGACGCTCGCGGCAGGCCAGCAGGTCTTGGT
GTTCCTCAACCGTCGCGGCTTTGCACCCACCTTGCTGTGCCACGACTGCGGGTGGATGTCCGGCTGCCAGCGCTGCGATG
CACGCATGACCGTGCACCAGCGCTCGGGCGAACTGCGCTGCCATCACTGCGGGTATGTCGAGCGTGTACCGCGCCAGTGT
CCGTCGTGCGGCAAGGTGGATTTGCGCCCGGTAGGCGCCGGCACCGAGCGCGCCGAAGAGCGCCTGGCGATTCTGTTTCC
AGACTACCCCGTGCTGCGTGTCGACCGCGACAGCACGTCGCGCAAGGACGCCATGAACCAGCTGTTCACGACGATCCAGC
GCGGCCAGCCATGCATTCTGGTCGGCACGCAAATGCTCGCCAAGGGCCATCACTTTCCCCGGGTCACGCTGGTATCGATT
CTGGACGCCGACGGCGGGCTGTTTTCCGGCGACTTCCGCGCCAGCGAGCGCATGGCGCAACTGATCGTTCAGGTGGCAGG
CCGTGCCGGGCGCGCCGAAGAACCTGGCAAGGTGATCATCCAGACCCACCTGGCCGACCATCCACTGCTGATTCAGCTGA
CCGAGCAGGGTTATTTCGCCTTTGCCGAACAGGCCTTGAGCGAGCGCCGTTCGGCGGGTCTGCCACCGTTTTCACACCTC
GCACTGCTGCGCGCCGAAGCGCACAAGCCGGGACAGGCCGAGGCCTTTCTCGATCAGGCCTGCAGCGATGCGGAGCAACT
GGTTGCGCAGATGGCGCTGGGCGGCATTGAGCTGCTGGGCCCGGTTCCGGCACCGATGGAACGCCGCGCCGGACGTTATC
GCGCGCAGTTGCTGGTGCAGTCCAGCGCCCGTGCGCCCTTGCATAAATTGCTTGCCACCTGGCTGCTCGCGCTGGAGCAA
ATGCCCAGCGGCCGGCAGGTCAGATGGTCGCTGGATGTGGATCCTGTGGATCTTTATTAA

Domains

Predicted by InterproScan.

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

DEAD/DEAH box helicase (DEAD)

(207-367)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.844

100

0.48

Multiple sequence alignment