Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   LLB26_RS10340 Genome accession   NZ_CP032435
Coordinates   2073979..2074425 (-) Length   148 a.a.
NCBI ID   WP_015082930.1    Uniprot ID   A0AAJ6N3H2
Organism   Lactococcus cremoris strain B26     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2069122..2073576 2073979..2074425 flank 403


Gene organization within MGE regions


Location: 2069122..2074425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLB26_RS10315 (LLB26_2032) - 2070582..2071391 (-) 810 WP_011677177.1 metal ABC transporter permease -
  LLB26_RS10320 (LLB26_2033) - 2071384..2072121 (-) 738 WP_015082929.1 metal ABC transporter ATP-binding protein -
  LLB26_RS10325 (LLB26_2034) - 2072300..2073142 (-) 843 WP_011677179.1 zinc ABC transporter substrate-binding protein -
  LLB26_RS10330 (LLB26_2035) - 2073139..2073576 (-) 438 WP_011677180.1 zinc-dependent MarR family transcriptional regulator -
  LLB26_RS10335 comGG 2073656..2073955 (-) 300 WP_011677181.1 competence type IV pilus minor pilin ComGG Machinery gene
  LLB26_RS10340 (LLB26_2036) comGF 2073979..2074425 (-) 447 WP_015082930.1 competence type IV pilus minor pilin ComGF Machinery gene

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 17160.85 Da        Isoelectric Point: 7.6669

>NTDB_id=315881 LLB26_RS10340 WP_015082930.1 2073979..2074425(-) (comGF) [Lactococcus cremoris strain B26]
MERKFCDLKLKIRAFTLLECLVALLAISGSVLVISGLTRMIEEQMKISQNDSRKDWQIFCEQMRSELSGEKLDNVNQNFL
YVTKDKKLRFGLVGDDFRKSDDKGQGYQPMLYDLKGAKIQAEENLIKITIDFDNGGERVFIYRFTDTK

Nucleotide


Download         Length: 447 bp        

>NTDB_id=315881 LLB26_RS10340 WP_015082930.1 2073979..2074425(-) (comGF) [Lactococcus cremoris strain B26]
ATGGAAAGGAAATTTTGCGACTTGAAGCTCAAAATTAGGGCATTTACTTTACTTGAATGTCTCGTAGCACTTTTGGCAAT
TTCTGGTTCAGTGCTAGTCATCTCAGGGCTGACTAGAATGATTGAAGAACAGATGAAAATAAGCCAGAATGATAGTAGGA
AAGATTGGCAAATATTTTGTGAACAAATGCGTTCAGAACTCTCCGGAGAAAAATTAGACAATGTGAATCAAAATTTTTTG
TATGTGACCAAAGATAAGAAGTTACGGTTCGGATTAGTAGGGGATGATTTTCGTAAAAGTGATGATAAAGGGCAAGGATA
CCAACCGATGCTTTATGATTTAAAAGGAGCAAAAATTCAGGCAGAAGAAAATTTGATAAAAATAACAATTGATTTTGATA
ATGGAGGTGAGCGAGTATTTATTTATCGATTTACTGATACAAAGTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Lactococcus lactis subsp. cremoris KW2

98.582

95.27

0.939
  comYF Streptococcus mutans UA140

44.681

95.27

0.426
  comYF Streptococcus mutans UA159

43.972

95.27

0.419
  comGF/cglF Streptococcus mitis SK321

39.286

94.595

0.372
  comGF/cglF Streptococcus mitis NCTC 12261

39.286

94.595

0.372

Multiple sequence alignment