Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   D3873_RS12705 Genome accession   NZ_CP032418
Coordinates   2429168..2430547 (-) Length   459 a.a.
NCBI ID   WP_119884353.1    Uniprot ID   A0A385YWI3
Organism   Paenisporosarcina cavernae strain K2R23-3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2424168..2435547
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D3873_RS12680 (D3873_12680) gltX 2424837..2426300 (-) 1464 WP_119884348.1 glutamate--tRNA ligase -
  D3873_RS12685 (D3873_12685) ispF 2426342..2426827 (-) 486 WP_119884349.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  D3873_RS12690 (D3873_12690) ispD 2426830..2427519 (-) 690 WP_119884350.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  D3873_RS12700 (D3873_12700) - 2428014..2429090 (-) 1077 WP_119884352.1 PIN/TRAM domain-containing protein -
  D3873_RS12705 (D3873_12705) radA 2429168..2430547 (-) 1380 WP_119884353.1 DNA repair protein RadA Machinery gene
  D3873_RS12710 (D3873_12710) clpC 2430608..2433055 (-) 2448 WP_119884354.1 ATP-dependent protease ATP-binding subunit ClpC -
  D3873_RS12715 (D3873_12715) - 2433045..2434145 (-) 1101 WP_119884355.1 protein arginine kinase -
  D3873_RS12720 (D3873_12720) - 2434142..2434681 (-) 540 WP_119884356.1 UvrB/UvrC motif-containing protein -
  D3873_RS12725 (D3873_12725) - 2434694..2435164 (-) 471 WP_119884357.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 50190.88 Da        Isoelectric Point: 6.8332

>NTDB_id=315686 D3873_RS12705 WP_119884353.1 2429168..2430547(-) (radA) [Paenisporosarcina cavernae strain K2R23-3]
MAKRKTKFMCNDCGYESPKWMGKCPGCGAWNTMVEEVEVVVKGPRRTFQHSQTVSAKATPITAIETQEEPRVATELNELN
RVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSALLANKGQRVLYMSGEESIRQTKLRAERLGVTSSELYIYSETNLELVNE
TIEQVEPKFVIIDSIQTVHHPEVTSAPGSVSQVRECTAELMRIAKTKNIAIFLVGHVTKEGQIAGPRLLEHMVDTVLYFE
GERHHTYRILRSQKNRFGSTNEIAIFEMLQGGLKEVLNPSELFLQERSQGAAGSTVVASMEGTRPILVEIQSLVTPSSFN
FPKRMASGIDQNRISLLMAVLEKRMGMLLQTQDAYIKVAGGVKLDEPAIDLAVLTSIVSSFKGTAVKATDCFIGEVGLTG
EIRRVSRIEQRVIEAAKLGFKKAIIPASNIGGWDFPEGIRVVGADTIDDALKEAFPYEG

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=315686 D3873_RS12705 WP_119884353.1 2429168..2430547(-) (radA) [Paenisporosarcina cavernae strain K2R23-3]
ATGGCAAAACGTAAAACGAAATTTATGTGTAATGACTGTGGCTACGAATCACCAAAATGGATGGGGAAATGCCCAGGTTG
TGGCGCTTGGAACACGATGGTAGAAGAAGTAGAAGTTGTCGTAAAAGGACCTCGCCGAACGTTCCAGCATAGCCAAACAG
TATCTGCGAAAGCAACGCCTATTACAGCAATTGAAACGCAAGAAGAGCCGCGAGTTGCAACGGAGTTAAACGAATTGAAT
CGCGTTCTTGGAGGAGGAATTGTGCCGGGTTCCCTCGTGCTTATTGGCGGAGACCCCGGGATTGGAAAGTCGACCCTACT
CCTTCAAGTAAGTGCTTTGCTTGCGAATAAAGGGCAGCGAGTCCTGTACATGTCAGGTGAAGAATCGATTCGCCAAACAA
AATTGAGGGCTGAGCGTTTAGGAGTTACCTCATCGGAGTTATATATTTATTCCGAAACAAACTTAGAATTGGTGAATGAA
ACCATCGAACAAGTAGAACCGAAATTTGTCATCATTGATTCGATCCAAACGGTTCATCACCCAGAAGTAACGAGTGCCCC
TGGTAGTGTGTCGCAAGTAAGAGAATGTACAGCGGAGTTAATGCGTATAGCAAAAACGAAGAATATTGCGATTTTCCTAG
TGGGACATGTCACAAAAGAAGGGCAAATTGCTGGTCCTAGATTGCTAGAACACATGGTGGATACCGTTCTTTATTTCGAA
GGGGAGCGCCATCATACGTATCGAATCTTACGCTCACAAAAGAACCGTTTTGGTTCTACGAACGAAATTGCGATATTTGA
GATGTTACAAGGTGGATTAAAGGAAGTATTAAATCCATCGGAATTATTTTTGCAAGAGCGTTCTCAAGGTGCCGCAGGAT
CGACAGTCGTGGCTTCAATGGAAGGGACGCGACCAATCCTTGTAGAGATACAATCCCTCGTCACGCCTTCTAGTTTTAAC
TTTCCAAAAAGGATGGCAAGTGGAATTGACCAAAATCGCATCTCGTTATTAATGGCAGTTCTCGAAAAACGAATGGGGAT
GCTTCTGCAGACGCAAGATGCCTATATTAAAGTAGCGGGTGGCGTAAAATTGGATGAACCTGCTATCGATTTGGCTGTAC
TGACGTCTATAGTATCGAGCTTTAAAGGAACTGCGGTAAAAGCGACGGATTGTTTTATTGGAGAAGTTGGTTTGACAGGA
GAAATTCGCCGAGTATCTCGGATTGAACAACGCGTAATAGAAGCGGCAAAACTCGGTTTTAAAAAAGCAATTATTCCTGC
ATCGAACATTGGTGGTTGGGACTTCCCAGAGGGGATTCGCGTCGTAGGTGCAGACACGATTGATGATGCGTTAAAAGAAG
CATTTCCTTATGAAGGGTGA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

AAA domain (AAA_25)

(70-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A385YWI3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

69.869

99.782

0.697
  radA Streptococcus pneumoniae Rx1

62.719

99.346

0.623
  radA Streptococcus pneumoniae D39

62.719

99.346

0.623
  radA Streptococcus pneumoniae R6

62.719

99.346

0.623
  radA Streptococcus pneumoniae TIGR4

62.719

99.346

0.623
  radA Streptococcus mitis NCTC 12261

62.719

99.346

0.623
  radA Streptococcus mitis SK321

62.719

99.346

0.623

Multiple sequence alignment