Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   D4Z93_RS01165 Genome accession   NZ_CP032416
Coordinates   238378..239733 (+) Length   451 a.a.
NCBI ID   WP_119969938.1    Uniprot ID   A0A386H0K7
Organism   Clostridium fermenticellae strain JN500901     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 233378..244733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D4Z93_RS01145 (D4Z93_01145) - 233652..234116 (+) 465 WP_119969934.1 CtsR family transcriptional regulator -
  D4Z93_RS01150 (D4Z93_01150) - 234140..234658 (+) 519 WP_119969935.1 UvrB/UvrC motif-containing protein -
  D4Z93_RS01155 (D4Z93_01155) - 234675..235703 (+) 1029 WP_119969936.1 protein arginine kinase -
  D4Z93_RS01160 (D4Z93_01160) - 235726..238170 (+) 2445 WP_119969937.1 ATP-dependent Clp protease ATP-binding subunit -
  D4Z93_RS01165 (D4Z93_01165) radA 238378..239733 (+) 1356 WP_119969938.1 DNA repair protein RadA Machinery gene
  D4Z93_RS01170 (D4Z93_01170) disA 239749..240807 (+) 1059 WP_119969939.1 DNA integrity scanning diadenylate cyclase DisA -
  D4Z93_RS01175 (D4Z93_01175) - 240861..241265 (-) 405 WP_119969940.1 DUF1573 domain-containing protein -
  D4Z93_RS01180 (D4Z93_01180) - 241753..242862 (+) 1110 WP_119969941.1 PIN/TRAM domain-containing protein -
  D4Z93_RS01185 (D4Z93_01185) ispD 242864..243577 (+) 714 WP_119969942.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 451 a.a.        Molecular weight: 49738.57 Da        Isoelectric Point: 8.2081

>NTDB_id=315641 D4Z93_RS01165 WP_119969938.1 238378..239733(+) (radA) [Clostridium fermenticellae strain JN500901]
MAKSKSVFVCQECGYESLKWLGKCPNCNTWNSMVEEVKEEKISTKQSVILNNEPRSIVSIKSGEYERLDTGISELNRVLG
GGIVKGSLTLISGAPGIGKSTLLLQAANNIAGRYGKVLYVSGEESEEQIKMRGDRLNVLSKDLYVISETNMDKIKEHITN
TNPVFVIIDSIQTLFKQSMTSAPGSVSQVRQNSNDIMSIGKTQNIPFFIVAHITKQGELAGPRVLEHMVDTVLSFEGERT
QEFRILRTIKNRFGNTSEIGVFEMSQEGLKQILNPSAVFLEEAGFQKEGSIVIGVIEGTRPILVEIQALVTETKAVMPRR
TAVGVDNLRLNLILAVLEKKLRVYFYNCDVYVNVVGGLNIEGTFGDLGLALALLSSVKSKAISLERLIVVGEIGLTGEVR
PVTFCDRLVNEAEKMGFKNAIIPYRNKDRIKNKNIDVISVSSLVEAINKVF

Nucleotide


Download         Length: 1356 bp        

>NTDB_id=315641 D4Z93_RS01165 WP_119969938.1 238378..239733(+) (radA) [Clostridium fermenticellae strain JN500901]
ATGGCAAAGAGCAAAAGTGTTTTTGTTTGTCAGGAGTGTGGATATGAATCATTAAAGTGGCTTGGAAAATGTCCCAATTG
TAATACATGGAACAGTATGGTGGAAGAAGTTAAAGAAGAAAAGATTTCTACGAAGCAGAGTGTAATTCTTAATAATGAAC
CACGTAGTATAGTAAGTATTAAATCAGGTGAATATGAAAGATTAGATACTGGAATATCTGAGTTAAACAGAGTTTTAGGA
GGAGGAATAGTTAAAGGATCTCTTACTTTGATCTCAGGAGCACCTGGAATAGGGAAATCAACTCTTCTTCTGCAGGCAGC
CAATAACATTGCGGGTAGATATGGTAAAGTTCTATATGTATCAGGAGAAGAATCTGAGGAACAGATAAAAATGCGTGGAG
ATAGATTAAATGTTCTATCAAAAGACCTTTACGTAATTTCAGAAACCAATATGGATAAAATAAAAGAACATATAACAAAT
ACTAATCCTGTATTTGTTATTATAGATTCAATACAAACACTTTTCAAACAATCAATGACTTCAGCGCCGGGAAGTGTATC
TCAAGTACGGCAAAATTCAAACGATATTATGAGTATAGGCAAAACTCAGAATATACCGTTTTTTATAGTAGCACACATAA
CTAAACAAGGAGAATTAGCAGGACCTAGGGTTTTAGAGCATATGGTTGATACTGTACTTTCCTTTGAAGGCGAGAGAACT
CAGGAATTTAGAATTCTTAGAACAATAAAAAATCGTTTTGGTAACACTAGTGAAATTGGTGTATTTGAGATGTCGCAAGA
GGGCCTTAAACAAATATTAAATCCGTCAGCTGTTTTTTTAGAAGAAGCGGGCTTTCAAAAAGAAGGCTCTATTGTAATAG
GTGTAATTGAAGGAACAAGACCAATACTTGTTGAAATACAAGCTCTTGTAACTGAAACTAAGGCAGTTATGCCAAGAAGA
ACGGCTGTTGGTGTGGATAATTTACGACTTAATTTAATACTAGCAGTTTTAGAAAAAAAATTAAGAGTTTACTTCTACAA
TTGTGATGTTTATGTTAATGTAGTAGGAGGATTAAATATAGAAGGAACATTTGGTGACCTGGGATTGGCATTAGCTCTTT
TGTCAAGTGTAAAGTCAAAGGCTATTTCACTTGAAAGGCTTATTGTAGTTGGGGAGATAGGTCTTACAGGAGAGGTTAGG
CCTGTGACATTTTGTGATAGGCTGGTAAATGAGGCAGAAAAGATGGGATTTAAAAATGCTATAATACCATACAGAAATAA
GGATAGAATAAAAAATAAGAATATTGACGTCATTAGTGTATCTTCCTTGGTTGAAGCAATAAATAAAGTTTTTTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(342-426)

AAA domain (AAA_25)

(59-217)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A386H0K7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.773

100

0.51
  radA Streptococcus pneumoniae Rx1

48.791

100

0.492
  radA Streptococcus pneumoniae D39

48.791

100

0.492
  radA Streptococcus pneumoniae R6

48.791

100

0.492
  radA Streptococcus pneumoniae TIGR4

48.791

100

0.492
  radA Streptococcus mitis NCTC 12261

48.791

100

0.492
  radA Streptococcus mitis SK321

48.571

100

0.49

Multiple sequence alignment