Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   E5D09_RS07020 Genome accession   NZ_CP038644
Coordinates   1440422..1441222 (-) Length   266 a.a.
NCBI ID   WP_004738086.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain ACN21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1435422..1446222
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5D09_RS07000 (E5D09_07000) - 1436487..1437185 (-) 699 WP_000058788.1 YfgM family protein -
  E5D09_RS07005 (E5D09_07005) hisS 1437214..1438506 (-) 1293 WP_000095254.1 histidine--tRNA ligase -
  E5D09_RS07010 (E5D09_07010) ispG 1438503..1439618 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  E5D09_RS07015 (E5D09_07015) - 1439640..1440431 (-) 792 WP_000405163.1 helix-turn-helix domain-containing protein -
  E5D09_RS07020 (E5D09_07020) pilF 1440422..1441222 (-) 801 WP_004738086.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  E5D09_RS07025 (E5D09_07025) rlmN 1441238..1442470 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  E5D09_RS07030 (E5D09_07030) ndk 1442593..1443024 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  E5D09_RS07035 (E5D09_07035) iscX 1443138..1443338 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  E5D09_RS07040 (E5D09_07040) - 1443438..1443989 (-) 552 WP_000669565.1 phosphatase PAP2 family protein -
  E5D09_RS07045 (E5D09_07045) - 1444014..1445312 (-) 1299 WP_086221435.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30094.44 Da        Isoelectric Point: 9.2307

>NTDB_id=315637 E5D09_RS07020 WP_004738086.1 1440422..1441222(-) (pilF) [Acinetobacter baumannii strain ACN21]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=315637 E5D09_RS07020 WP_004738086.1 1440422..1441222(-) (pilF) [Acinetobacter baumannii strain ACN21]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCTTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterProScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

100

100

1
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726