Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   D5F53_RS00990 Genome accession   NZ_CP032412
Coordinates   223647..225014 (+) Length   455 a.a.
NCBI ID   WP_009594669.1    Uniprot ID   A0A1R1AQC1
Organism   Paenibacillus lautus strain E7593-69     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 218647..230014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5F53_RS00970 (D5F53_00970) - 218965..219489 (+) 525 WP_119846243.1 hypothetical protein -
  D5F53_RS00975 (D5F53_00975) - 219528..220592 (+) 1065 WP_119846244.1 protein arginine kinase -
  D5F53_RS00980 (D5F53_00980) clpC 220712..223168 (+) 2457 WP_096777181.1 ATP-dependent protease ATP-binding subunit ClpC -
  D5F53_RS00985 (D5F53_00985) - 223316..223522 (-) 207 WP_119846245.1 hypothetical protein -
  D5F53_RS00990 (D5F53_00990) radA 223647..225014 (+) 1368 WP_009594669.1 DNA repair protein RadA Machinery gene
  D5F53_RS00995 (D5F53_00995) disA 225028..226104 (+) 1077 WP_009594677.1 DNA integrity scanning diadenylate cyclase DisA -
  D5F53_RS01000 (D5F53_01000) pssA 226154..226888 (+) 735 WP_228468840.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  D5F53_RS01005 (D5F53_01005) - 227015..227410 (-) 396 WP_009594665.1 hypothetical protein -
  D5F53_RS01010 (D5F53_01010) - 227691..228779 (+) 1089 WP_015737636.1 PIN/TRAM domain-containing protein -
  D5F53_RS01015 (D5F53_01015) ispD 228857..229552 (+) 696 WP_119846246.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49595.35 Da        Isoelectric Point: 7.4121

>NTDB_id=315588 D5F53_RS00990 WP_009594669.1 223647..225014(+) (radA) [Paenibacillus lautus strain E7593-69]
MAKTKTKFFCTECGYEAPKWFGKCPGCQSWNSMVEETETVIKTQGMNSPLFHSKEKPLSIINIESGKEPRIQTGIAELNR
VLGGGLVPGSLVLVGGDPGIGKSTLLLQASNRLAQNGIRVLYISGEESVRQTKLRADRLGALSPELFVLCETNLESIEEA
IENVQPGFVVIDSIQTVYLPEVTSAPGSVSQVRECTARFMRLAKVKGIATVLVGHVTKEGAIAGPRMLEHMVDCVLYFEG
ERHHTYRLLRAVKNRFGSTNEIGIFEMGEDGLREVGNPSELFLSERPLGVAGSTVVASMEGTRPVLVELQALISTTHFPS
PRRMGTGVDHHRMNLIIAVLEKRMGMYLQTQDAYLNVAGGVKLDEPAVDLAIAVSIASSFRDIPTKPYDVIFGEVGLTGE
VRAVSRAEQRVREAEKLGFKRVLMPEKSLKGWKHPKGIQIIGVNTVADALAVALD

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=315588 D5F53_RS00990 WP_009594669.1 223647..225014(+) (radA) [Paenibacillus lautus strain E7593-69]
ATGGCAAAAACTAAGACGAAATTTTTCTGTACCGAATGCGGCTATGAAGCGCCCAAATGGTTCGGAAAATGTCCGGGCTG
CCAATCATGGAATTCCATGGTAGAGGAGACCGAGACGGTAATCAAGACCCAGGGGATGAATTCCCCTCTTTTTCATAGTA
AAGAAAAACCCCTTTCGATCATAAATATAGAAAGCGGTAAAGAACCCAGAATCCAGACGGGCATTGCGGAATTGAACCGC
GTGCTCGGGGGCGGTCTGGTCCCGGGCTCGCTCGTGCTAGTCGGCGGCGATCCCGGCATCGGCAAATCCACGCTCCTGCT
TCAAGCCTCCAACCGATTGGCCCAGAACGGCATTCGCGTTCTGTACATTTCCGGTGAGGAATCCGTGCGCCAGACCAAAC
TGCGCGCCGACCGGCTCGGGGCGCTATCCCCGGAGCTGTTTGTCCTCTGCGAAACCAATCTGGAATCGATCGAGGAAGCC
ATTGAGAACGTACAGCCTGGCTTCGTTGTCATTGACTCCATTCAGACGGTGTACTTGCCTGAGGTAACCAGCGCGCCGGG
CAGCGTATCCCAGGTTCGGGAATGCACGGCCCGCTTTATGCGGCTGGCGAAGGTGAAGGGCATTGCGACCGTCCTTGTCG
GACATGTGACAAAAGAAGGCGCCATTGCAGGCCCAAGGATGCTGGAGCACATGGTGGACTGTGTGCTTTATTTTGAAGGA
GAGCGTCATCATACTTACCGCTTGCTTCGCGCGGTGAAGAATCGTTTCGGTTCGACGAATGAGATTGGCATTTTTGAGAT
GGGTGAGGATGGGCTTCGGGAAGTCGGGAATCCATCGGAACTCTTTTTATCCGAACGGCCTTTGGGCGTAGCGGGTTCGA
CGGTAGTTGCAAGCATGGAGGGGACGCGTCCGGTTCTGGTAGAGCTGCAGGCGCTTATCTCCACGACGCACTTCCCGTCA
CCGCGCCGGATGGGCACAGGGGTAGACCATCACCGCATGAACCTAATCATTGCCGTGCTGGAGAAACGGATGGGGATGTA
CCTGCAAACCCAAGACGCCTACCTGAACGTAGCGGGCGGCGTGAAGCTCGATGAACCTGCGGTGGATCTGGCCATAGCGG
TCAGCATCGCGTCCAGCTTCCGCGACATACCGACCAAGCCGTACGACGTTATTTTTGGCGAGGTGGGACTGACCGGCGAG
GTTCGCGCCGTTTCAAGAGCGGAGCAGAGGGTAAGAGAAGCCGAGAAGCTGGGTTTTAAACGCGTTCTCATGCCCGAGAA
AAGTTTGAAGGGATGGAAGCATCCGAAAGGAATACAAATTATCGGAGTCAACACCGTTGCAGATGCATTAGCGGTTGCGT
TAGATTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(344-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-35)

AAA domain (AAA_25)

(70-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R1AQC1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.885

100

0.675
  radA Streptococcus pneumoniae Rx1

59.111

98.901

0.585
  radA Streptococcus pneumoniae D39

59.111

98.901

0.585
  radA Streptococcus pneumoniae R6

59.111

98.901

0.585
  radA Streptococcus pneumoniae TIGR4

59.111

98.901

0.585
  radA Streptococcus mitis NCTC 12261

59.111

98.901

0.585
  radA Streptococcus mitis SK321

58.889

98.901

0.582

Multiple sequence alignment