Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   D5F52_RS20115 Genome accession   NZ_CP032410
Coordinates   4286968..4288332 (-) Length   454 a.a.
NCBI ID   WP_119734230.1    Uniprot ID   -
Organism   Brevibacillus laterosporus strain E7593-50     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4281968..4293332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5F52_RS20090 (D5F52_20090) ispD 4282428..4283129 (-) 702 WP_104067885.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  D5F52_RS20095 (D5F52_20095) - 4283160..4284251 (-) 1092 WP_104033118.1 PIN/TRAM domain-containing protein -
  D5F52_RS20100 (D5F52_20100) - 4284558..4284950 (+) 393 WP_094701829.1 DUF1573 domain-containing protein -
  D5F52_RS20105 (D5F52_20105) pssA 4285051..4285770 (-) 720 WP_018673922.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  D5F52_RS20110 (D5F52_20110) disA 4285912..4286958 (-) 1047 WP_018673923.1 DNA integrity scanning diadenylate cyclase DisA -
  D5F52_RS20115 (D5F52_20115) radA 4286968..4288332 (-) 1365 WP_119734230.1 DNA repair protein RadA Machinery gene
  D5F52_RS20120 (D5F52_20120) - 4288523..4290985 (-) 2463 WP_119734231.1 ATP-dependent Clp protease ATP-binding subunit -
  D5F52_RS20125 (D5F52_20125) - 4291013..4292077 (-) 1065 WP_018673926.1 protein arginine kinase -
  D5F52_RS20130 (D5F52_20130) - 4292104..4292640 (-) 537 WP_094701833.1 UvrB/UvrC motif-containing protein -
  D5F52_RS20135 (D5F52_20135) - 4292716..4293180 (-) 465 WP_018673928.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49620.14 Da        Isoelectric Point: 6.1351

>NTDB_id=315570 D5F52_RS20115 WP_119734230.1 4286968..4288332(-) (radA) [Brevibacillus laterosporus strain E7593-50]
MSKVKTKFVCQDCGYESPKWMGKCPGCNNWNTLVEELERKQVGGRERGMSTKQKAQPITEVISDEEPRLDTTLGELNRVL
GGGLVLGSLVLVGGDPGIGKSTLLLQTSFALAHQNNKVLYISGEESVKQIKMRADRLGVQTSNLLVVTETDLEQVEEQIT
FVDPDVVIIDSIQTIFHPAVTSAAGSVAQVRECTSHLMRIAKSKGIAIFIVGHVTKEGAIAGPRMLEHMVDAVLYFEGDR
HNTFRILRAVKNRFGSTNEMGIFEMKEKGLTEVENPSELFLAERPIGVAGSTVVASMEGTRPVLVELQALVSPTSFATPR
RMATGVDHQRIAMIMAVLEKRVGLMLQNQDAYVNVAGGVRLDEPAIDLAIAVSIASSFRDHSTNPYDVVIGEIGLTGEVR
GVSRIEQRIREAEKLGFKRVIIPERSMRGLENPSGIEVIGVKNVEEALEYALRG

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=315570 D5F52_RS20115 WP_119734230.1 4286968..4288332(-) (radA) [Brevibacillus laterosporus strain E7593-50]
ATGTCAAAAGTAAAAACAAAGTTTGTGTGTCAGGATTGTGGGTACGAAAGTCCCAAATGGATGGGCAAATGCCCAGGCTG
TAATAATTGGAATACACTGGTTGAAGAATTAGAACGTAAACAAGTAGGTGGCAGAGAGCGTGGAATGTCCACCAAACAAA
AAGCACAACCAATCACAGAGGTAATAAGTGACGAAGAACCACGATTAGATACCACTTTAGGTGAATTAAACCGTGTATTA
GGTGGCGGATTAGTTTTGGGATCACTGGTGCTTGTTGGAGGAGACCCGGGGATCGGAAAGTCTACTTTACTTCTCCAAAC
TTCTTTTGCTCTCGCACATCAGAATAATAAAGTGTTGTATATCTCCGGTGAGGAGTCTGTTAAACAAATTAAAATGCGAG
CTGATCGACTTGGTGTGCAAACATCAAATCTGCTAGTAGTGACAGAGACTGACTTGGAGCAGGTGGAGGAGCAGATAACG
TTTGTAGACCCTGATGTGGTCATAATTGACTCAATCCAAACCATTTTTCATCCTGCTGTCACATCTGCAGCAGGTAGTGT
AGCTCAGGTCAGAGAGTGTACCAGCCACCTCATGCGAATAGCAAAAAGTAAAGGTATTGCTATCTTTATTGTCGGACATG
TTACAAAAGAAGGAGCAATTGCTGGACCGCGTATGCTTGAGCATATGGTTGATGCTGTTCTTTACTTTGAAGGAGATCGA
CATAATACCTTCCGTATTTTACGGGCAGTTAAGAACCGATTTGGATCTACAAATGAAATGGGTATTTTTGAAATGAAAGA
AAAAGGACTTACTGAAGTAGAAAATCCTTCTGAACTATTCTTAGCCGAACGTCCGATTGGAGTTGCTGGATCAACAGTAG
TAGCGAGTATGGAAGGGACTCGCCCTGTACTTGTTGAACTACAGGCATTGGTTTCCCCAACCAGCTTTGCTACTCCACGT
CGAATGGCAACAGGAGTAGATCATCAGCGAATTGCTATGATTATGGCCGTTTTGGAGAAACGAGTAGGCTTGATGCTACA
AAACCAAGATGCCTATGTAAATGTAGCTGGTGGTGTTCGTCTGGATGAGCCTGCCATTGATTTAGCTATTGCAGTGAGCA
TTGCTAGTAGTTTTCGAGATCATTCTACAAATCCATATGACGTAGTGATTGGAGAAATTGGTTTAACAGGTGAAGTACGC
GGTGTTTCTCGAATTGAGCAACGTATACGTGAAGCGGAAAAACTGGGCTTTAAGCGTGTCATTATTCCGGAGAGAAGCAT
GCGAGGACTAGAAAATCCTTCAGGCATTGAGGTAATTGGTGTGAAGAATGTAGAGGAAGCTTTGGAATATGCGTTGAGGG
GGTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(68-218)

Subunit ChlI of Mg-chelatase (ChlI)

(340-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

68.777

100

0.694
  radA Streptococcus mitis NCTC 12261

59.111

99.119

0.586
  radA Streptococcus pneumoniae Rx1

59.111

99.119

0.586
  radA Streptococcus pneumoniae D39

59.111

99.119

0.586
  radA Streptococcus pneumoniae R6

59.111

99.119

0.586
  radA Streptococcus pneumoniae TIGR4

59.111

99.119

0.586
  radA Streptococcus mitis SK321

59.111

99.119

0.586

Multiple sequence alignment