Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoS   Type   Regulator
Locus tag   E5F07_RS20525 Genome accession   NZ_CP038494
Coordinates   4203985..4204977 (-) Length   330 a.a.
NCBI ID   WP_000081550.1    Uniprot ID   A0A370V765
Organism   Escherichia coli O157:H7 strain TT12B     
Function   regulation of chitinases (predicted from homology)   
Competence regulation

Genomic Context

Location: 4198985..4209977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5F07_RS20500 (E5F07_21090) pphB 4200347..4201003 (+) 657 WP_001141350.1 protein-serine/threonine phosphatase -
  E5F07_RS20505 (E5F07_21095) - 4201044..4201280 (-) 237 WP_000562982.1 non-oxidative hydroxyarylic acid decarboxylases subunit D -
  E5F07_RS20510 (E5F07_21100) - 4201291..4202718 (-) 1428 WP_000863205.1 non-oxidative hydroxyarylic acid decarboxylases subunit C -
  E5F07_RS20515 (E5F07_21105) - 4202718..4203311 (-) 594 WP_000767724.1 non-oxidative hydroxyarylic acid decarboxylases subunit B -
  E5F07_RS20520 (E5F07_21110) - 4203458..4203865 (+) 408 WP_000175365.1 MarR family transcriptional regulator -
  E5F07_RS20525 (E5F07_21115) rpoS 4203985..4204977 (-) 993 WP_000081550.1 RNA polymerase sigma factor RpoS Regulator
  E5F07_RS20530 (E5F07_21120) nlpD 4205040..4206134 (-) 1095 WP_306256364.1 murein hydrolase activator NlpD -
  E5F07_RS20535 (E5F07_21125) pcm 4206319..4206945 (-) 627 WP_000254708.1 protein-L-isoaspartate O-methyltransferase -
  E5F07_RS20540 (E5F07_21130) surE 4206939..4207700 (-) 762 WP_001295182.1 5'/3'-nucleotidase SurE -
  E5F07_RS20545 (E5F07_21135) truD 4207681..4208730 (-) 1050 WP_000568934.1 tRNA pseudouridine(13) synthase TruD -
  E5F07_RS20550 (E5F07_21140) ispF 4208727..4209206 (-) 480 WP_001219242.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  E5F07_RS20555 (E5F07_21145) ispD 4209206..4209916 (-) 711 WP_000246158.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 37972.84 Da        Isoelectric Point: 4.5597

>NTDB_id=315259 E5F07_RS20525 WP_000081550.1 4203985..4204977(-) (rpoS) [Escherichia coli O157:H7 strain TT12B]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide


Download         Length: 993 bp        

>NTDB_id=315259 E5F07_RS20525 WP_000081550.1 4203985..4204977(-) (rpoS) [Escherichia coli O157:H7 strain TT12B]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGTGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACAGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTACTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

Sigma-70 region 3 (Sigma70_r3)

(174-248)

Sigma-70 region 2 (Sigma70_r2)

(94-163)

Sigma-70 factor, region 1.2 (Sigma70_r1_2)

(56-88)

Sigma-70, region 4 (Sigma70_r4)

(262-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A370V765

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoS Vibrio cholerae O1 biovar El Tor strain E7946

73.065

97.879

0.715