Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   D4A37_RS20755 Genome accession   NZ_CP032365
Coordinates   4029841..4030275 (-) Length   144 a.a.
NCBI ID   WP_000811502.1    Uniprot ID   A0A5C5ACH0
Organism   Bacillus wiedmannii strain SR52     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4024841..4035275
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D4A37_RS20735 - 4025249..4025806 (+) 558 WP_000062072.1 PadR family transcriptional regulator -
  D4A37_RS20740 - 4025998..4026429 (-) 432 WP_000438217.1 NUDIX hydrolase -
  D4A37_RS20745 metE 4027090..4029378 (+) 2289 WP_001007588.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  D4A37_RS20750 comJ 4029416..4029805 (-) 390 WP_000424013.1 competence protein ComJ -
  D4A37_RS20755 nucA/comI 4029841..4030275 (-) 435 WP_000811502.1 DNA-entry nuclease Machinery gene
  D4A37_RS20760 - 4030513..4031262 (+) 750 WP_000387497.1 DUF3967 domain-containing protein -
  D4A37_RS20765 kinB 4031315..4032589 (-) 1275 WP_000420379.1 sporulation sensor histidine kinase KinB -
  D4A37_RS20770 - 4032899..4033099 (+) 201 WP_000929285.1 hypothetical protein -
  D4A37_RS20775 - 4033223..4034386 (+) 1164 WP_000434595.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16010.38 Da        Isoelectric Point: 8.4331

>NTDB_id=315202 D4A37_RS20755 WP_000811502.1 4029841..4030275(-) (nucA/comI) [Bacillus wiedmannii strain SR52]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=315202 D4A37_RS20755 WP_000811502.1 4029841..4030275(-) (nucA/comI) [Bacillus wiedmannii strain SR52]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTGTATGAAGTACTTGTTCC
AGAAGAAACAAGTATCAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGCGATATCCAGAGACAGGGA
AACATATTACCGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGACCGTGGTGGCGCTGCGGATAGAAGAAAA
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCAGCCGATAATCGCGGGGCAGGGTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACACGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5C5ACH0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

71.171

77.083

0.549

Multiple sequence alignment