Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   D4A81_RS04490 Genome accession   NZ_CP032364
Coordinates   978597..979961 (+) Length   454 a.a.
NCBI ID   WP_111525712.1    Uniprot ID   A0A385Q328
Organism   Lachnoanaerobaculum umeaense strain DSM 23576 = CCUG 58757     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 973597..984961
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D4A81_RS04470 (D4A81_04470) - 974223..974411 (+) 189 WP_111525681.1 hypothetical protein -
  D4A81_RS04475 (D4A81_04475) - 974469..976256 (+) 1788 WP_111525680.1 leucine-rich repeat domain-containing protein -
  D4A81_RS04480 (D4A81_04480) - 976296..977984 (+) 1689 WP_111525679.1 leucine-rich repeat domain-containing protein -
  D4A81_RS04485 (D4A81_04485) - 978132..978542 (+) 411 WP_111525678.1 endosialidase -
  D4A81_RS04490 (D4A81_04490) radA 978597..979961 (+) 1365 WP_111525712.1 DNA repair protein RadA Machinery gene
  D4A81_RS04495 (D4A81_04495) ybaK 980051..980518 (+) 468 WP_111525677.1 Cys-tRNA(Pro) deacylase -
  D4A81_RS04500 (D4A81_04500) - 980528..983137 (+) 2610 WP_111525676.1 translation factor GTPase family protein -
  D4A81_RS04505 (D4A81_04505) - 983189..983329 (-) 141 WP_111525675.1 FeoB-associated Cys-rich membrane protein -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49821.94 Da        Isoelectric Point: 7.4162

>NTDB_id=315165 D4A81_RS04490 WP_111525712.1 978597..979961(+) (radA) [Lachnoanaerobaculum umeaense strain DSM 23576 = CCUG 58757]
MAKSKSSAFFCKECGYESPKWLGQCPMCKAWNSLIEEPVVKAKTVSRGISSNTYKKPARLSEIQLEDEDRFKTGFEELDR
VLGGGVVRGSLVLVGGDPGIGKSTLLLQVCKNISDDERSVLYISGEESLKQIKMRAKRVGEIKGDLSFLCETNLNIIKET
VLSEKPDVVVIDSIQTMFNEEVASAPGSVSQVRESTNLLMQTAKENNIAIFIVGHVTKEGQVAGPRVLEHMVDTVLYFEG
DRFASYRILRAVKNRFGSTNEIGVFEMQEVGLIEVKNPSEFMLSGRPQNATGAAVACLMEGSRPILVEVQALVVPTAFGL
PRRTSTGTDVNRINLLMAVIEKRCALAMSRYDAYINIAGGLRINEPALDLSIIMSLISSMKNRTIDEKTVIFGEVGLSGE
VRAVSMPEIRIREAMKLGFEVCILPIVCLEKLSFKGNIKLIGVRNINEAVSLLS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=315165 D4A81_RS04490 WP_111525712.1 978597..979961(+) (radA) [Lachnoanaerobaculum umeaense strain DSM 23576 = CCUG 58757]
ATGGCAAAATCAAAGTCCAGTGCATTCTTTTGTAAGGAATGTGGATATGAATCACCTAAGTGGCTTGGACAATGTCCTAT
GTGCAAAGCTTGGAATAGCCTAATAGAAGAGCCGGTGGTGAAAGCGAAAACTGTTAGCAGAGGAATATCATCAAATACAT
ATAAAAAGCCGGCCAGATTATCAGAAATACAGCTGGAAGATGAAGACAGATTTAAGACAGGCTTTGAAGAGCTGGATAGA
GTACTAGGAGGTGGCGTAGTAAGAGGTTCACTTGTATTGGTAGGTGGAGATCCCGGTATAGGAAAATCTACATTATTGCT
TCAAGTATGTAAGAATATTTCAGATGATGAAAGATCAGTCCTTTATATCTCAGGAGAGGAAAGCTTAAAGCAGATAAAGA
TGAGAGCAAAAAGAGTTGGAGAAATCAAGGGAGATTTGAGCTTTCTATGTGAAACCAATCTGAATATAATAAAAGAAACA
GTACTTAGTGAGAAACCGGATGTTGTGGTGATAGACTCTATACAGACTATGTTTAATGAGGAAGTGGCTTCAGCACCCGG
AAGTGTAAGCCAGGTAAGGGAGTCTACAAATCTTCTTATGCAGACAGCAAAAGAAAATAATATTGCAATATTTATTGTAG
GCCATGTTACAAAAGAAGGGCAGGTTGCAGGTCCTAGAGTGCTTGAACATATGGTAGATACAGTGCTTTATTTTGAGGGA
GATAGATTTGCCTCATATAGGATACTTCGTGCAGTTAAGAATAGATTCGGTTCTACAAATGAAATAGGTGTGTTTGAGAT
GCAGGAGGTAGGCCTTATAGAGGTCAAAAACCCTTCTGAGTTTATGTTATCAGGTAGACCACAAAATGCTACAGGTGCAG
CTGTAGCTTGCCTTATGGAGGGGAGTCGACCTATTTTGGTAGAGGTACAGGCTTTGGTAGTACCTACTGCCTTCGGACTT
CCAAGGAGGACTTCTACAGGAACGGATGTGAACAGAATCAATCTCTTGATGGCTGTCATAGAAAAAAGATGTGCTTTGGC
TATGAGTAGATATGATGCCTATATAAATATTGCAGGTGGACTTAGGATAAATGAGCCGGCATTGGATCTTTCTATAATAA
TGTCTCTTATTTCAAGTATGAAGAATAGAACAATAGATGAAAAGACTGTGATATTCGGAGAGGTAGGACTTTCAGGAGAG
GTTAGAGCTGTAAGTATGCCGGAAATCAGAATAAGAGAGGCTATGAAGCTTGGATTTGAGGTATGCATACTTCCAATAGT
ATGCCTCGAAAAGCTTTCATTTAAAGGCAATATAAAGCTAATAGGAGTAAGGAATATAAATGAAGCTGTTTCATTATTGA
GTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(61-220)

Subunit ChlI of Mg-chelatase (ChlI)

(344-425)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(10-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A385Q328

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

55.188

99.78

0.551
  radA Streptococcus pneumoniae Rx1

53.556

99.119

0.531
  radA Streptococcus pneumoniae D39

53.556

99.119

0.531
  radA Streptococcus pneumoniae R6

53.556

99.119

0.531
  radA Streptococcus pneumoniae TIGR4

53.556

99.119

0.531
  radA Streptococcus mitis SK321

53.556

99.119

0.531
  radA Streptococcus mitis NCTC 12261

53.556

99.119

0.531

Multiple sequence alignment