Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   D3X12_RS00110 Genome accession   NZ_CP032358
Coordinates   26285..27652 (-) Length   455 a.a.
NCBI ID   WP_011063580.1    Uniprot ID   A0A2C9ETR8
Organism   Pseudomonas protegens strain pf5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 21285..32652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D3X12_RS00085 (D3X12_00085) - 22133..22330 (-) 198 WP_007937254.1 YbdD/YjiX family protein -
  D3X12_RS00090 (D3X12_00090) - 22347..24413 (-) 2067 WP_011063576.1 carbon starvation CstA family protein -
  D3X12_RS00095 (D3X12_00095) - 24630..25091 (+) 462 WP_015637107.1 isoprenylcysteine carboxylmethyltransferase family protein -
  D3X12_RS00100 (D3X12_00100) - 25146..25514 (+) 369 WP_011063578.1 PilZ domain-containing protein -
  D3X12_RS00105 (D3X12_00105) - 25522..26250 (-) 729 WP_019095099.1 hypothetical protein -
  D3X12_RS00110 (D3X12_00110) radA 26285..27652 (-) 1368 WP_011063580.1 DNA repair protein RadA Machinery gene
  D3X12_RS00115 (D3X12_00115) - 27698..28258 (-) 561 WP_011063581.1 ankyrin repeat domain-containing protein -
  D3X12_RS00120 (D3X12_00120) katB 28325..29866 (-) 1542 WP_011063582.1 catalase KatB -
  D3X12_RS00125 (D3X12_00125) mscL 30154..30567 (+) 414 WP_011063583.1 large-conductance mechanosensitive channel protein MscL -
  D3X12_RS00130 (D3X12_00130) - 30615..31391 (-) 777 WP_011063584.1 ferredoxin--NADP reductase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48740.27 Da        Isoelectric Point: 7.1317

>NTDB_id=315111 D3X12_RS00110 WP_011063580.1 26285..27652(-) (radA) [Pseudomonas protegens strain pf5]
MAKAKRMYGCTECGSTFPKWAGQCGECGAWNTLTETMIESGGAAAPAGRTGWAGQQAQIKTLAEVSVEEIPRFSTASGEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIATRMPALYVTGEESQQQVAMRARRLGLPQDQLRVMTETCIETIIA
TARVEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDRGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHLA
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPPGLKIIAVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=315111 D3X12_RS00110 WP_011063580.1 26285..27652(-) (radA) [Pseudomonas protegens strain pf5]
ATGGCCAAGGCCAAGCGCATGTACGGCTGCACCGAGTGCGGCTCCACCTTTCCCAAATGGGCCGGGCAGTGCGGTGAGTG
CGGGGCCTGGAACACCCTGACCGAGACCATGATCGAAAGCGGCGGTGCGGCGGCACCTGCCGGGCGCACCGGCTGGGCCG
GGCAGCAGGCGCAGATCAAGACCCTGGCGGAAGTCAGCGTCGAGGAAATTCCGCGTTTCTCCACCGCTTCCGGTGAACTC
GATCGGGTACTGGGCGGCGGCCTGGTGGACGGTTCGGTGGTGCTGATCGGTGGTGATCCGGGGATCGGCAAGTCGACCAT
CCTGCTGCAGACCCTGTGCAATATCGCCACGCGCATGCCGGCGCTGTATGTCACCGGTGAGGAATCCCAGCAGCAGGTGG
CGATGCGCGCCCGGCGCCTGGGCCTGCCCCAGGACCAACTGCGGGTGATGACCGAGACCTGCATCGAAACCATCATCGCC
ACGGCCCGGGTAGAAAAACCCAAGGTCATGGTGATCGACTCGATCCAGACGATTTTCACCGAACAACTGCAGTCGGCCCC
GGGCGGCGTGTCCCAGGTGCGCGAGAGCGCGGCCTTGCTGGTGCGCTACGCCAAGCAGAGCGGCACGGCGATCTTCCTCG
TCGGCCACGTGACCAAGGAAGGCGCCCTGGCCGGGCCGCGGGTGCTGGAGCACATGGTGGACACCGTGCTGTATTTCGAA
GGCGAATCCGACGGGCGCCTGCGCTTGCTGCGGGCGGTGAAGAACCGTTTCGGTGCGGTGAATGAGCTGGGTGTATTCGG
CATGACTGACCGCGGATTGAAGGAAGTCTCCAATCCTTCGGCGATTTTCCTCACTCGTGCCCAGGAAGAGGTTCCGGGCA
GCGTGGTGATGGCCACCTGGGAAGGCACCCGGCCGATGCTGGTGGAGGTCCAGGCGCTGGTGGATGACAGCCACTTGGCC
AACCCGCGCCGGGTGACCCTGGGCCTGGACCAGAACCGCCTGGCCATGCTGCTGGCGGTGCTGCATCGCCATGGCGGCAT
CCCCACCCACGATCAGGATGTGTTCCTCAACGTGGTGGGCGGGGTCAAGGTGCTGGAAACCGCCTCCGACCTGGCGTTGA
TGGCCGCCGTGATGTCGAGCCTGCGCAACCGTCCGTTGCCCCATGACCTGCTGGTGTTTGGCGAAGTCGGCCTGTCGGGT
GAGGTGCGCCCGGTGCCCAGCGGTCAGGAGCGCTTGAAGGAAGCGGCCAAGCACGGCTTCAAGCGAGCTATCGTGCCCAA
GGGCAACGCGCCGAAAGAGGCGCCGCCGGGCTTGAAGATCATTGCCGTGACCCGCCTGGAGCAGGCGCTGGATGCACTTT
TCGAGTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)

AAA domain (AAA_25)

(80-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2C9ETR8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.234

100

0.495
  radA Streptococcus pneumoniae Rx1

45.87

100

0.464
  radA Streptococcus pneumoniae R6

45.87

100

0.464
  radA Streptococcus pneumoniae TIGR4

45.87

100

0.464
  radA Streptococcus pneumoniae D39

45.87

100

0.464
  radA Streptococcus mitis SK321

46.053

100

0.462
  radA Streptococcus mitis NCTC 12261

45.833

100

0.459

Multiple sequence alignment