Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   E4T63_RS26535 Genome accession   NZ_CP038438
Coordinates   5723568..5725100 (-) Length   510 a.a.
NCBI ID   WP_135296764.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain LBUM677     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5718568..5730100
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4T63_RS26505 (E4T63_26505) thiS 5719259..5719459 (-) 201 WP_167734067.1 sulfur carrier protein ThiS -
  E4T63_RS26510 (E4T63_26510) - 5719540..5719911 (-) 372 WP_003229144.1 DUF423 domain-containing protein -
  E4T63_RS26515 (E4T63_26515) mtgA 5719986..5720708 (+) 723 WP_135296763.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  E4T63_RS26520 (E4T63_26520) rpoH 5720908..5721762 (-) 855 WP_003229146.1 RNA polymerase sigma factor RpoH -
  E4T63_RS26525 (E4T63_26525) ftsX 5721878..5722903 (-) 1026 WP_007964135.1 permease-like cell division protein FtsX -
  E4T63_RS26530 (E4T63_26530) ftsE 5722900..5723571 (-) 672 WP_003229148.1 cell division ATP-binding protein FtsE -
  E4T63_RS26535 (E4T63_26535) pilA 5723568..5725100 (-) 1533 WP_135296764.1 signal recognition particle-docking protein FtsY Machinery gene
  E4T63_RS26540 (E4T63_26540) - 5725445..5726800 (+) 1356 WP_025112091.1 pitrilysin family protein -
  E4T63_RS26545 (E4T63_26545) - 5726793..5728280 (+) 1488 WP_135296765.1 pitrilysin family protein -
  E4T63_RS26550 (E4T63_26550) rsmD 5728280..5728885 (+) 606 WP_134784882.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  E4T63_RS26560 (E4T63_26560) - 5728999..5729997 (+) 999 WP_135296766.1 hydrolase -

Sequence

Protein


Download         Length: 510 a.a.        Molecular weight: 53743.06 Da        Isoelectric Point: 4.5935

>NTDB_id=314931 E4T63_RS26535 WP_135296764.1 5723568..5725100(-) (pilA) [Pseudomonas fluorescens strain LBUM677]
MFGSNDDKKTPAAAGEKKSLFGWLRKKPQEPVVEQPPAIPEPAPTPAPAIEEEPAPIVLPIAEPVLQPVEPEPESETPVA
AELPLTPAAEPWLTLPVAEEPVALVEEAAPHVTPPIPAPAAFVPEVAPAPVVEPVVEPAPAVPEPVTPVVVAPVAPVAQQ
PEPAPAPAPAPAPAPVVAAPVVPVEVVAETPVEAPRSEETKVGFFARLKQGLSKTSASIGEGMASLFLGRKTIDDDLLDE
LETRLLTADVGVEATTQIIQRLTQKVARKELADADALYKSLQAELAAMLKPVEQPLKIASQNKPFVILVVGVNGAGKTTT
IGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNKIPVIAQHTGADSASVIFDAVQAAKARGIDVLIADTAGRLHT
KDNLMEELKKVRRVIGKLDADAPHEVLLVLDAGTGQNAINQAKQFNQTVELTGLALTKLDGTAKGGVIFALAKQFGLPIR
YIGVGEGIDDLRTFEAEPFVQALFAERERS

Nucleotide


Download         Length: 1533 bp        

>NTDB_id=314931 E4T63_RS26535 WP_135296764.1 5723568..5725100(-) (pilA) [Pseudomonas fluorescens strain LBUM677]
ATGTTTGGTTCCAACGACGACAAGAAGACCCCAGCTGCGGCTGGCGAGAAGAAAAGCCTGTTCGGATGGCTGCGTAAGAA
ACCGCAGGAACCCGTCGTCGAACAGCCGCCCGCGATTCCTGAACCGGCTCCGACGCCAGCGCCTGCTATAGAAGAAGAGC
CGGCGCCGATCGTGCTGCCGATTGCCGAGCCGGTGCTGCAACCGGTCGAACCCGAGCCAGAATCTGAAACCCCGGTGGCG
GCCGAGCTGCCACTGACTCCGGCGGCCGAGCCGTGGCTGACCTTGCCGGTGGCGGAAGAACCGGTGGCCTTGGTCGAAGA
GGCCGCGCCGCATGTGACGCCGCCGATTCCTGCACCTGCCGCGTTCGTCCCTGAAGTCGCTCCGGCGCCAGTGGTTGAGC
CGGTTGTCGAGCCTGCGCCGGCTGTTCCCGAGCCAGTGACCCCGGTGGTCGTCGCCCCTGTTGCGCCGGTCGCTCAGCAG
CCCGAGCCTGCACCTGCACCTGCACCTGCACCTGCACCTGCACCTGTCGTCGCTGCTCCGGTGGTGCCGGTAGAAGTGGT
TGCTGAAACGCCAGTGGAAGCCCCGCGCAGCGAAGAAACCAAAGTCGGCTTCTTCGCCCGCCTCAAGCAAGGCCTGTCGA
AAACCAGCGCCAGCATCGGCGAGGGCATGGCCAGCCTGTTTCTCGGCCGTAAAACCATTGATGACGATTTGCTCGATGAG
CTGGAAACCCGTCTGCTGACCGCCGACGTCGGTGTCGAAGCCACCACGCAGATCATCCAGCGCCTGACTCAGAAGGTTGC
ACGCAAAGAGTTGGCTGACGCCGACGCGCTGTACAAGTCGTTGCAGGCCGAGCTGGCGGCGATGCTCAAGCCGGTCGAGC
AGCCGCTGAAAATTGCCTCGCAGAACAAGCCGTTCGTGATTCTGGTGGTCGGCGTCAATGGCGCCGGCAAGACCACCACC
ATTGGCAAGCTGGCGAAGAAGCTGCAACTGGAAGGCAAGAAAGTCATGCTCGCCGCCGGTGACACCTTCCGCGCCGCTGC
CGTTGAGCAGCTGCAGGTCTGGGGCGAGCGCAACAAGATCCCGGTGATTGCCCAGCACACCGGTGCCGACTCTGCTTCGG
TGATCTTCGACGCCGTGCAGGCCGCCAAGGCCCGTGGCATCGACGTGTTGATCGCCGACACCGCCGGTCGTCTGCACACC
AAAGACAACCTGATGGAAGAGCTGAAGAAAGTTCGCCGGGTGATCGGCAAGCTCGACGCCGACGCACCGCACGAAGTGCT
GCTGGTACTTGATGCCGGCACCGGTCAGAACGCGATCAACCAGGCCAAGCAATTCAACCAGACCGTCGAACTGACCGGCC
TGGCGCTGACCAAGCTCGACGGTACCGCCAAGGGCGGGGTGATTTTCGCCCTGGCCAAGCAGTTTGGTCTGCCGATCCGC
TACATCGGTGTCGGTGAAGGCATCGACGATCTGCGTACCTTTGAAGCCGAACCCTTTGTCCAGGCACTGTTTGCCGAGCG
GGAGCGTTCATGA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(220-285)

SRP54-type protein, GTPase domain (SRP54)

(304-504)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

49.005

78.824

0.386