Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CDG62_RS05310 Genome accession   NZ_CP032286
Coordinates   520573..521373 (+) Length   266 a.a.
NCBI ID   WP_087528037.1    Uniprot ID   A0A385N680
Organism   Acinetobacter sp. WCHA55     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 515573..526373
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG62_RS05280 (CDG62_05285) - 515783..516199 (+) 417 WP_087528033.1 hypothetical protein -
  CDG62_RS05285 (CDG62_05290) - 516340..517620 (+) 1281 WP_087528034.1 glycosyltransferase family 4 protein -
  CDG62_RS05290 (CDG62_05295) - 517813..518364 (+) 552 WP_087528035.1 phosphatase PAP2 family protein -
  CDG62_RS05295 (CDG62_05300) iscX 518455..518655 (+) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  CDG62_RS05300 (CDG62_05305) ndk 518766..519197 (+) 432 WP_004697669.1 nucleoside-diphosphate kinase -
  CDG62_RS05305 (CDG62_05310) rlmN 519320..520555 (+) 1236 WP_087528036.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  CDG62_RS05310 (CDG62_05315) pilF 520573..521373 (+) 801 WP_087528037.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  CDG62_RS05315 (CDG62_05320) - 521364..522140 (+) 777 WP_087528038.1 helix-turn-helix domain-containing protein -
  CDG62_RS05320 (CDG62_05325) ispG 522159..523280 (+) 1122 WP_086210453.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  CDG62_RS05325 (CDG62_05330) hisS 523284..524579 (+) 1296 WP_087528039.1 histidine--tRNA ligase -
  CDG62_RS05330 (CDG62_05335) - 524607..525305 (+) 699 WP_087528040.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30078.02 Da        Isoelectric Point: 8.8903

>NTDB_id=314739 CDG62_RS05310 WP_087528037.1 520573..521373(+) (pilF) [Acinetobacter sp. WCHA55]
MKKPVSKLYLALTLGVSVWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNVEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLQVAGSTLGYDQRYRALENLGRAYIQVGRV
TEAEAAFKQALQVNSGAYLAMIEMAEISYLRQQNAEATQYYEQFVQAVGEKNLDARALWIGIRIARANHDTMGSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=314739 CDG62_RS05310 WP_087528037.1 520573..521373(+) (pilF) [Acinetobacter sp. WCHA55]
TTGAAAAAACCCGTTTCTAAACTTTACCTTGCACTGACACTGGGTGTGTCAGTGTGGTACTTGGTGGGTTGCCAAACCGT
GAATACGAAAGGTGACCCAAGTAAAGCAGTACAAGTTAGAACGCAATTGGCGGCTGAATATATTAAGTCGAATGATTACG
ATGCTGCGAAACGCACATTGGATCAGGCATTAGATATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGCGTGTTG
TTACAGCGTGAAGGCAGTCCACAAAATGTGGAAAAGGCAGAGCGTTATTTTAAGCATGCCATTGCGGCAGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCAAGTTG
CAGGTTCAACTTTAGGGTATGATCAACGTTATCGAGCATTGGAAAATTTAGGTCGTGCGTATATACAAGTCGGTCGAGTG
ACGGAAGCAGAAGCTGCTTTTAAACAAGCCCTACAGGTAAACTCAGGTGCCTATCTTGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCAAAATGCTGAAGCTACGCAATACTATGAACAGTTTGTGCAAGCGGTTGGCGAGAAAAACTTAG
ATGCACGTGCGCTTTGGATTGGGATCCGAATTGCGCGGGCAAATCATGACACGATGGGTTCGCAAGTACTGGTCAACCAG
CTACGTGCGTTGTATCCAGACAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_19)

(89-147)

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-205)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A385N680

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

67.537

100

0.68
  pilF Acinetobacter baylyi ADP1

63.534

100

0.635

Multiple sequence alignment