Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   BEN74_RS08285 Genome accession   NZ_CP032279
Coordinates   1400723..1401526 (-) Length   267 a.a.
NCBI ID   WP_068907797.1    Uniprot ID   A0A3B7PRH8
Organism   Acinetobacter sp. WCHAc010034     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1395723..1406526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEN74_RS08265 (BEN74_08265) - 1396782..1397480 (-) 699 WP_068907789.1 YfgM family protein -
  BEN74_RS08270 (BEN74_08270) hisS 1397511..1398806 (-) 1296 WP_068907791.1 histidine--tRNA ligase -
  BEN74_RS08275 (BEN74_08275) ispG 1398803..1399918 (-) 1116 WP_068907793.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  BEN74_RS08280 (BEN74_08280) - 1399941..1400732 (-) 792 WP_068907795.1 helix-turn-helix domain-containing protein -
  BEN74_RS08285 (BEN74_08285) pilF 1400723..1401526 (-) 804 WP_068907797.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  BEN74_RS08290 (BEN74_08290) rlmN 1401541..1402773 (-) 1233 WP_068907799.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  BEN74_RS08295 (BEN74_08295) ndk 1402897..1403328 (-) 432 WP_004647460.1 nucleoside-diphosphate kinase -
  BEN74_RS08300 (BEN74_08300) iscX 1403441..1403641 (-) 201 WP_068907801.1 Fe-S cluster assembly protein IscX -
  BEN74_RS08305 (BEN74_08305) - 1403730..1404281 (-) 552 WP_068907803.1 phosphatase PAP2 family protein -
  BEN74_RS08310 (BEN74_08310) - 1404318..1405589 (-) 1272 WP_068907804.1 glycosyltransferase family 4 protein -
  BEN74_RS08315 (BEN74_08315) - 1405733..1406149 (-) 417 WP_068907806.1 hypothetical protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 29908.00 Da        Isoelectric Point: 9.4398

>NTDB_id=314698 BEN74_RS08285 WP_068907797.1 1400723..1401526(-) (pilF) [Acinetobacter sp. WCHAc010034]
MRTLRYKLAFISSLSLAALALTACQTPDLGKKDPEKAAKVRTQLAVEYIKAGDLDAAKRALDQALETQPRDAQANMMMGV
LLQQEGSPLNMEKADSYFRRAISAEPKNAQARNNYGTYLYQIERYNDALEQLNIAGATLGYDQRARALENAGRIYLKLGD
AANAEKSFKQALQANRDSFISMLELAEIFYLNQQTAAATQMYEQFVRAVGQKNQSARALWIGIRTARANGDSLGMQALVN
QLRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=314698 BEN74_RS08285 WP_068907797.1 1400723..1401526(-) (pilF) [Acinetobacter sp. WCHAc010034]
TTGAGAACCTTAAGGTATAAACTTGCTTTCATTTCATCTTTAAGCCTTGCAGCTTTGGCGCTGACGGCCTGCCAGACCCC
AGATCTCGGCAAAAAAGATCCGGAGAAGGCAGCCAAAGTGCGCACTCAATTGGCGGTTGAATACATTAAGGCCGGCGACC
TGGACGCGGCCAAGCGCGCGCTGGATCAGGCGCTGGAAACGCAGCCGCGCGACGCCCAAGCCAATATGATGATGGGCGTG
CTGCTGCAGCAGGAAGGCAGCCCGCTGAATATGGAAAAAGCCGACAGCTATTTCCGGCGCGCCATTTCCGCAGAACCGAA
AAATGCGCAGGCGCGTAACAACTATGGTACGTATTTGTATCAAATTGAGCGCTATAATGACGCGCTTGAACAGCTGAACA
TCGCCGGCGCGACACTGGGCTATGATCAGCGCGCGCGCGCGCTGGAAAATGCCGGCCGGATTTACCTGAAGCTGGGCGAT
GCCGCGAATGCTGAAAAATCATTCAAGCAGGCCCTGCAGGCCAATCGAGATTCCTTCATTTCAATGTTAGAGTTGGCGGA
AATTTTTTATTTAAACCAGCAAACCGCTGCCGCGACGCAAATGTATGAACAGTTTGTGCGCGCAGTCGGGCAGAAAAACC
AGAGCGCCCGCGCGCTCTGGATTGGCATCCGCACTGCGCGTGCGAATGGGGACAGTCTGGGCATGCAGGCGCTGGTCAAC
CAGCTGCGGGCGCTGTTCCCTGAAAGTCCCGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAA
GTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_14)

(41-80)

Tetratricopeptide repeat (TPR_16)

(91-134)

Tetratricopeptide repeat (TPR_8)

(145-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3B7PRH8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

75.655

100

0.757
  pilF Acinetobacter baylyi ADP1

71.429

94.382

0.674

Multiple sequence alignment