Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   D3H65_RS20950 Genome accession   NZ_CP032157
Coordinates   5566478..5567851 (+) Length   457 a.a.
NCBI ID   WP_119052187.1    Uniprot ID   A0A3B7MTD5
Organism   Paraflavitalea soli strain 5GH32-13     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5561478..5572851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D3H65_RS20935 (D3H65_20935) - 5562401..5563966 (+) 1566 WP_245999551.1 Rne/Rng family ribonuclease -
  D3H65_RS20945 (D3H65_20945) - 5565165..5565857 (-) 693 WP_119052186.1 zinc metallopeptidase -
  D3H65_RS20950 (D3H65_20950) radA 5566478..5567851 (+) 1374 WP_119052187.1 DNA repair protein RadA Machinery gene
  D3H65_RS20955 (D3H65_20955) - 5567965..5568663 (+) 699 WP_119052188.1 ComF family protein -
  D3H65_RS20960 (D3H65_20960) - 5568761..5570155 (+) 1395 WP_119052189.1 choice-of-anchor Q domain-containing protein -
  D3H65_RS20965 (D3H65_20965) - 5570274..5570789 (+) 516 WP_119052190.1 glutathione peroxidase -
  D3H65_RS20970 (D3H65_20970) - 5571267..5572490 (+) 1224 WP_119052191.1 DNA polymerase III subunit delta' -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 51002.73 Da        Isoelectric Point: 6.6956

>NTDB_id=314224 D3H65_RS20950 WP_119052187.1 5566478..5567851(+) (radA) [Paraflavitalea soli strain 5GH32-13]
MSKVKSAFFCSNCGYESAKWLGKCPSCGSWNTFVQEVIHKETSQKENNWKNFHEDKKEIKTIALHAVTTPEQRRIITPDA
ELNRVLGGGIVPGSITLIAGEPGIGKSTLFLQIGLLLKDITILYISGEESDQQIKMRADRLKIQNDQFYLLTETSTQTIF
QEIKKLRPQLVIVDSIQTIQSPYIDSSPGSISQIRESASEFQRFAKETNIPVFLIGHITKDGAIAGPKILEHMVDTVLQF
EGDRHYAYRILRTIKNRFGGTAELGIYEMSGDGMRGVNNPSEILITQKEDRLSGIAIAATIEGMRPLLIEVQALVTQSVY
GTPQRTVSGFDLRRMQLLLAVLEKRGGFHFGVKDVFLNIAGGLKVEDPSIDLAVLCALLSSYEDVPLPDLTCFAGEVGLS
GEIRAVHRIEQRIAEAEKLGFEKILVSRYNQKGLNLQKFNIEVISMGRVDEVYQYLF

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=314224 D3H65_RS20950 WP_119052187.1 5566478..5567851(+) (radA) [Paraflavitalea soli strain 5GH32-13]
ATGAGTAAAGTGAAATCAGCATTCTTTTGTAGCAACTGTGGATATGAAAGCGCTAAATGGCTCGGCAAATGTCCTTCATG
TGGTTCCTGGAATACTTTTGTGCAGGAAGTCATACATAAAGAGACTTCCCAAAAAGAGAATAACTGGAAAAACTTTCACG
AAGACAAAAAAGAGATCAAAACAATTGCACTGCATGCAGTAACAACACCCGAACAAAGACGCATCATCACACCCGATGCA
GAATTGAACAGGGTGCTGGGTGGAGGTATCGTTCCCGGGAGCATCACTTTGATAGCCGGCGAACCCGGCATTGGTAAATC
AACCTTGTTCTTACAGATCGGATTGTTATTAAAAGACATTACCATCCTATACATCAGCGGCGAAGAGAGTGACCAGCAGA
TCAAAATGCGGGCAGACCGTTTAAAAATACAGAACGATCAGTTTTACCTGCTCACAGAAACATCTACACAAACCATTTTC
CAGGAAATTAAAAAGCTTCGGCCACAACTCGTTATTGTTGACTCTATTCAAACCATTCAATCACCTTACATAGATTCATC
ACCGGGCAGCATTTCCCAGATACGTGAATCAGCCTCAGAGTTTCAACGCTTTGCCAAAGAAACCAACATACCTGTATTCC
TCATTGGTCATATCACCAAAGACGGCGCCATAGCGGGTCCCAAGATATTGGAGCATATGGTAGATACCGTATTACAGTTT
GAAGGAGACCGCCACTATGCATACCGCATCCTACGTACTATTAAGAACCGTTTCGGCGGAACAGCAGAGCTCGGCATTTA
TGAAATGTCGGGCGATGGCATGCGCGGCGTTAACAATCCCAGCGAAATACTCATTACACAAAAAGAGGACCGGCTCAGCG
GCATTGCTATTGCCGCCACTATTGAAGGCATGCGTCCCTTACTCATAGAAGTACAGGCACTGGTCACCCAATCTGTTTAT
GGAACACCCCAGCGCACCGTATCCGGTTTCGATCTCCGCCGCATGCAACTGCTGCTGGCTGTATTGGAAAAGCGCGGAGG
CTTTCATTTTGGGGTCAAAGATGTATTTCTCAACATTGCCGGTGGTCTTAAGGTAGAAGATCCCTCCATTGATCTCGCTG
TCCTCTGCGCCCTGTTAAGCTCTTATGAAGATGTGCCCCTCCCCGATCTTACCTGTTTTGCCGGAGAGGTAGGATTAAGC
GGTGAGATCAGAGCTGTTCATCGTATTGAACAACGCATTGCCGAAGCTGAGAAACTGGGATTTGAAAAGATCCTCGTATC
ACGTTACAATCAGAAAGGCCTTAACCTGCAGAAATTCAATATCGAAGTGATCAGCATGGGAAGAGTAGATGAAGTATACC
AATACCTGTTTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-35)

Subunit ChlI of Mg-chelatase (ChlI)

(344-430)

AAA domain (AAA_25)

(73-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3B7MTD5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae R6

49.563

100

0.497
  radA Streptococcus pneumoniae Rx1

49.563

100

0.497
  radA Streptococcus pneumoniae D39

49.563

100

0.497
  radA Streptococcus pneumoniae TIGR4

49.563

100

0.497
  radA Streptococcus mitis NCTC 12261

49.345

100

0.495
  radA Streptococcus mitis SK321

49.127

100

0.492
  radA Bacillus subtilis subsp. subtilis str. 168

48.246

99.781

0.481

Multiple sequence alignment