Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LL1196_RS11810 Genome accession   NZ_CP032148
Coordinates   2269027..2270388 (-) Length   453 a.a.
NCBI ID   WP_011677138.1    Uniprot ID   A0A165G707
Organism   Lactococcus cremoris strain 1196     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2246129..2271264 2269027..2270388 within 0


Gene organization within MGE regions


Location: 2246129..2271264
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LL1196_RS11680 (LL1196_2343) spxB 2246500..2248232 (-) 1733 Protein_2272 pyruvate oxidase -
  LL1196_RS11685 (LL1196_2345) - 2248479..2249435 (-) 957 WP_011677116.1 serine hydrolase domain-containing protein -
  LL1196_RS11690 (LL1196_2346) - 2249508..2250287 (-) 780 WP_011677117.1 CppA C-terminal domain-containing protein -
  LL1196_RS11695 (LL1196_2347) - 2250518..2250679 (-) 162 WP_014573297.1 hypothetical protein -
  LL1196_RS11700 (LL1196_2348) - 2250751..2251080 (-) 330 WP_011677119.1 iron chaperone -
  LL1196_RS11705 (LL1196_2349) - 2251115..2251621 (-) 507 WP_011677120.1 pyridoxamine 5'-phosphate oxidase family protein -
  LL1196_RS11710 (LL1196_2350) - 2251990..2252772 (+) 783 WP_011677121.1 CPBP family intramembrane glutamic endopeptidase -
  LL1196_RS11715 (LL1196_2351) gla 2252808..2253677 (-) 870 WP_011677122.1 aquaglyceroporin Gla -
  LL1196_RS11720 (LL1196_2352) - 2253849..2256140 (+) 2292 WP_021165010.1 Xaa-Pro dipeptidyl-peptidase -
  LL1196_RS13745 - 2256142..2256853 (+) 712 Protein_2281 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
  LL1196_RS11735 (LL1196_2354) - 2257003..2257845 (+) 843 WP_011677124.1 amino acid ABC transporter substrate-binding protein -
  LL1196_RS11740 (LL1196_2355) - 2257908..2258609 (-) 702 WP_021212002.1 FAD-dependent oxidoreductase -
  LL1196_RS11745 (LL1196_2356) gltX 2258721..2260172 (-) 1452 WP_021216281.1 glutamate--tRNA ligase -
  LL1196_RS11750 (LL1196_2357) - 2260314..2260808 (-) 495 WP_015082904.1 cysteine hydrolase family protein -
  LL1196_RS11755 (LL1196_2358) - 2260812..2261354 (-) 543 WP_015082905.1 cysteine hydrolase family protein -
  LL1196_RS11760 (LL1196_2359) - 2261463..2262347 (+) 885 WP_021216280.1 LysR family transcriptional regulator -
  LL1196_RS11765 (LL1196_2360) - 2262380..2263066 (-) 687 WP_031289126.1 phosphatase PAP2 family protein -
  LL1196_RS11770 (LL1196_2361) - 2263542..2264036 (+) 495 WP_021212004.1 GNAT family N-acetyltransferase -
  LL1196_RS11775 (LL1196_2362) - 2264127..2264450 (-) 324 WP_021212005.1 DUF1048 domain-containing protein -
  LL1196_RS11780 (LL1196_2363) - 2264447..2264785 (-) 339 WP_011677133.1 DUF1048 domain-containing protein -
  LL1196_RS11785 (LL1196_2364) - 2264778..2265134 (-) 357 WP_011677134.1 PadR family transcriptional regulator -
  LL1196_RS11790 (LL1196_2365) - 2265257..2265886 (-) 630 WP_011677135.1 hypothetical protein -
  LL1196_RS11795 (LL1196_2366) - 2265944..2267029 (-) 1086 WP_021216279.1 PIN/TRAM domain-containing protein -
  LL1196_RS11800 (LL1196_2367) - 2267296..2267979 (+) 684 WP_015082909.1 YoaK family protein -
  LL1196_RS11805 (LL1196_2368) - 2268036..2268926 (+) 891 WP_205536420.1 IS982-like element IS982B family transposase -
  LL1196_RS11810 (LL1196_2369) radA 2269027..2270388 (-) 1362 WP_011677138.1 DNA repair protein RadA Machinery gene

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49897.33 Da        Isoelectric Point: 5.2965

>NTDB_id=314096 LL1196_RS11810 WP_011677138.1 2269027..2270388(-) (radA) [Lactococcus cremoris strain 1196]
MAKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMSGERSKPMKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIKVIGVTTLTECLKLVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=314096 LL1196_RS11810 WP_011677138.1 2269027..2270388(-) (radA) [Lactococcus cremoris strain 1196]
ATGGCGAAGAAAAAATCATCTTTTGTTTGTCAAAATTGTGGTTATAAATCTGCAAAGTATCTTGGGCGATGCCCAAATTG
TGGAGAGTGGTCCTCTTTTGTTGAAGAAGTTGAAGTACAGGAAGTCAAAAATCAGCGAGTGTCGATGTCTGGTGAACGTT
CAAAACCAATGAAATTAGATGAAGTTGAGCTTTTTGATACACCACGTATTGAAACAGATCTTGACGAATTTAATCGTGTT
TTAGGTGGTGGTGTTGTTCCTGGTTCACTCGTTTTGATTGGTGGAGACCCAGGAATAGGAAAATCTACGTTGCTTTTGCA
AGTATCGACTCAGCTTGCATCAAGAGGACGGGTTCTCTATGTGAGTGGTGAAGAATCTGCTCAACAAATTAAGCTTAGAG
CGGAACGTTTGGGCGATATTGATCGAGATTTTTATCTTTACGCCGAGACTAACATGCAGTCAATTCGTGCTGAAGTTGAA
CGTTTGAAGCCAAATTTTTTGATTATTGATTCGATCCAAACTATTATGACACCTGAAATTCAATCAACTCAAGGCTCTGT
GAGTCAAGTTCGAGAAGTGACAGGTGAACTCATGCAAATTGCCAAAACGAATGATATTGCTACTTTTATCGTTGGTCATG
TGACGAAAGAAGGTCAATTGGCAGGACCAAGAATGCTAGAGCACATGGTTGATACCGTTCTTTATTTTGAAGGAGAAAGA
AATAATACTTTTAGAATTCTTCGAGCAGTTAAAAACCGGTTTGGGTCAACCAATGAAATTGGTATATTCGAAATGCAAGG
GCATGGTTTAGTTGAAGTAACTAATCCTTCTGAGGTTTTCTTGGAAGAGCGATTAGAGGGTTCAACTGGTTCTGCTATTG
TTTGTGCACTCGAAGGGACACGTCCGATATTAGTTGAAATACAGGCTTTAACTACACCAACAATGTTTGGAAATGCTAAG
AGAACAACTTCTGGTTTAGATTTTAATAGAGTGAGCCTGATTATGGCAGTACTTGAAAAACGGACAGGCTTATTGATGCA
AAATCAAGATGCTTACCTTAAATCAGCTGGGGGAGTAAAATTAGATGAGCCAGCCATTGACCTTGCGGTTGCTGTCGCTG
TAGCCTCAAGTTATAAAGAACTTCCGACGGATGCGCGTGAATGTTTTATTGGTGAAATTGGCTTAACAGGAGAGATTCGT
CGTGTGACGCGAATTGAACAAAGACTGAATGAAGCTGCTAAATTAGGATTCAAAAAAGTCTATGCTCCTAAAAATTCAAT
TGTGGGAATTGATATTCCTGAACAAATAAAAGTAATTGGGGTAACAACCCTAACTGAATGTCTAAAATTAGTATTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A165G707

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.015

100

0.81
  radA Streptococcus pneumoniae D39

81.015

100

0.81
  radA Streptococcus pneumoniae R6

81.015

100

0.81
  radA Streptococcus pneumoniae TIGR4

81.015

100

0.81
  radA Streptococcus mitis SK321

81.015

100

0.81
  radA Streptococcus mitis NCTC 12261

80.795

100

0.808
  radA Bacillus subtilis subsp. subtilis str. 168

61.707

100

0.623

Multiple sequence alignment