Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   E4U87_RS09860 Genome accession   NZ_CP038402
Coordinates   2013782..2015206 (-) Length   474 a.a.
NCBI ID   WP_000132546.1    Uniprot ID   -
Organism   Escherichia coli O157:H7 strain BB24-1     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2008782..2020206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4U87_RS09830 (E4U87_10050) yeeZ 2008837..2009661 (+) 825 WP_000754765.1 SDR family oxidoreductase -
  E4U87_RS09835 (E4U87_10055) yeeY 2009707..2010636 (+) 930 WP_000803334.1 LysR substrate-binding domain-containing protein -
  E4U87_RS09840 (E4U87_10060) yoeI 2010851..2010913 (+) 63 WP_010723108.1 membrane protein YoeI -
  E4U87_RS09845 (E4U87_10065) plaP 2010903..2012261 (+) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  E4U87_RS09850 (E4U87_10070) tsuA 2012440..2013498 (+) 1059 WP_000492327.1 thiosulfate utilization transporter TsuA/YeeE -
  E4U87_RS09855 (E4U87_10075) tsuB 2013512..2013739 (+) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  E4U87_RS09860 (E4U87_10080) sbcB 2013782..2015206 (-) 1425 WP_000132546.1 exodeoxyribonuclease I Machinery gene
  E4U87_RS09865 (E4U87_10085) - 2015388..2016566 (+) 1179 WP_001007946.1 site-specific integrase -
  E4U87_RS09870 (E4U87_10090) - 2016547..2016738 (-) 192 WP_000132739.1 AlpA family phage regulatory protein -
  E4U87_RS09875 (E4U87_10095) - 2016816..2017160 (-) 345 WP_001281188.1 hypothetical protein -
  E4U87_RS09880 (E4U87_10100) - 2017348..2017698 (-) 351 WP_000610373.1 DUF551 domain-containing protein -
  E4U87_RS09885 - 2017695..2018051 (-) 357 Protein_1955 hypothetical protein -
  E4U87_RS09890 (E4U87_10110) - 2018385..2018563 (-) 179 Protein_1956 hypothetical protein -
  E4U87_RS09895 (E4U87_10115) - 2018565..2019512 (-) 948 WP_001289930.1 ead/Ea22-like family protein -
  E4U87_RS09900 (E4U87_10120) - 2019509..2019730 (-) 222 WP_000763383.1 TraR/DksA family transcriptional regulator -
  E4U87_RS09905 (E4U87_10125) - 2019829..2020044 (-) 216 WP_000188870.1 hypothetical protein -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 54397.63 Da        Isoelectric Point: 5.2440

>NTDB_id=314012 E4U87_RS09860 WP_000132546.1 2013782..2015206(-) (sbcB) [Escherichia coli O157:H7 strain BB24-1]
MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENE
AAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWRG
NTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDAD
RLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVDK
RIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=314012 E4U87_RS09860 WP_000132546.1 2013782..2015206(-) (sbcB) [Escherichia coli O157:H7 strain BB24-1]
ATGACAGACACAGATAAGCAACCTACCTTCCTCTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCC
TGCACAGTTCGCAGCCATTCGCACCGATGACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCGG
ATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCTCGGGCGAAAGGAGAAAACGAA
GCCGCATTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGA
CGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCT
GGGATTTACTGGATGTTATGCGTGCCTGCTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGTCTA
CCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGTGTA
CGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAACCACGCCTGTTTGATTATCTCTTTACCCATCGTAATAAAC
ACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGCGGC
AATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGATAT
TTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCG
CCGTTCCGGTTAAACTGGTACATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGAC
CGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGTGGT
GGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAATGGCTTTTTCAGTGACG
CCGATCGTGCAGCAATGAAAATTGTGCTTGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGATAAA
CGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCT
GGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTACAAGGCTATGCAGAAGAGATTCAGATGCTGGCGCAGCAGTATG
CCGACGATAAAGAAAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATTGTCTAA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(12-187)

Exonuclease C-terminal (ExoI_SH3)

(211-471)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.936

99.156

0.584