Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   E3154_RS09695 Genome accession   NZ_CP038398
Coordinates   1994626..1996050 (-) Length   474 a.a.
NCBI ID   WP_000132546.1    Uniprot ID   -
Organism   Escherichia coli O157:H7 strain DEC4E     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1989626..2001050
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3154_RS09665 (E3154_09880) yeeZ 1989681..1990505 (+) 825 WP_000754737.1 SDR family oxidoreductase -
  E3154_RS09670 (E3154_09885) yeeY 1990551..1991480 (+) 930 WP_000803334.1 LysR substrate-binding domain-containing protein -
  E3154_RS09675 (E3154_09890) yoeI 1991695..1991757 (+) 63 WP_010723108.1 membrane protein YoeI -
  E3154_RS09680 (E3154_09895) plaP 1991747..1993105 (+) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  E3154_RS09685 (E3154_09900) tsuA 1993284..1994342 (+) 1059 WP_000492327.1 thiosulfate utilization transporter TsuA/YeeE -
  E3154_RS09690 (E3154_09905) tsuB 1994356..1994583 (+) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  E3154_RS09695 (E3154_09910) sbcB 1994626..1996050 (-) 1425 WP_000132546.1 exodeoxyribonuclease I Machinery gene
  E3154_RS09700 (E3154_09915) - 1996232..1997410 (+) 1179 WP_001007946.1 site-specific integrase -
  E3154_RS09705 (E3154_09920) - 1997391..1997582 (-) 192 WP_000132739.1 AlpA family phage regulatory protein -
  E3154_RS09710 (E3154_09925) - 1997660..1997950 (-) 291 WP_096980766.1 hypothetical protein -
  E3154_RS09715 (E3154_09930) - 1998011..1999224 (+) 1214 WP_162829202.1 IS3-like element IS1203 family transposase -
  E3154_RS09720 (E3154_09940) - 1999261..1999428 (+) 168 Protein_1925 phage tail fiber C-terminal domain-containing protein -
  E3154_RS09725 (E3154_09945) - 1999430..1999699 (+) 270 WP_001023353.1 phage tail fiber C-terminal domain-containing protein -
  E3154_RS09730 (E3154_09950) - 1999840..2000715 (+) 876 WP_000491542.1 hypothetical protein -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 54397.63 Da        Isoelectric Point: 5.2440

>NTDB_id=313935 E3154_RS09695 WP_000132546.1 1994626..1996050(-) (sbcB) [Escherichia coli O157:H7 strain DEC4E]
MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENE
AAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWRG
NTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDAD
RLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVDK
RIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=313935 E3154_RS09695 WP_000132546.1 1994626..1996050(-) (sbcB) [Escherichia coli O157:H7 strain DEC4E]
ATGACAGACACAGATAAGCAACCTACCTTCCTCTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCC
TGCACAGTTCGCAGCCATTCGCACCGATGACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCGG
ATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCTCGGGCGAAAGGAGAAAACGAA
GCCGCATTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGA
CGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCT
GGGATTTACTGGATGTTATGCGTGCCTGCTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGTCTA
CCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGTGTA
CGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAACCACGCCTGTTTGATTATCTCTTTACCCATCGTAATAAAC
ACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGCGGC
AATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGATAT
TTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCG
CCGTTCCGGTTAAACTGGTACATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGAC
CGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGTGGT
GGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAATGGCTTTTTCAGTGACG
CCGATCGTGCAGCAATGAAAATTGTGCTTGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGATAAA
CGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCT
GGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTACAAGGCTATGCAGAAGAGATTCAGATGCTGGCGCAGCAGTATG
CCGACGATAAAGAAAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATTGTCTAA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(12-187)

Exonuclease C-terminal (ExoI_SH3)

(211-471)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.936

99.156

0.584