Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CDG61_RS03340 Genome accession   NZ_CP032143
Coordinates   605469..606317 (+) Length   282 a.a.
NCBI ID   WP_087551349.1    Uniprot ID   -
Organism   Acinetobacter sp. WCHAc010052     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 600469..611317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG61_RS03310 (CDG61_03310) - 600511..601320 (+) 810 WP_087551378.1 XAC2610-related protein -
  CDG61_RS03315 (CDG61_03315) - 601423..602709 (+) 1287 WP_087551352.1 glycosyltransferase family 4 protein -
  CDG61_RS03320 (CDG61_03320) - 602742..603293 (+) 552 WP_087551351.1 phosphatase PAP2 family protein -
  CDG61_RS03325 (CDG61_03325) iscX 603384..603584 (+) 201 WP_087513069.1 Fe-S cluster assembly protein IscX -
  CDG61_RS03330 (CDG61_03330) ndk 603706..604137 (+) 432 WP_087513068.1 nucleoside-diphosphate kinase -
  CDG61_RS03335 (CDG61_03335) rlmN 604259..605494 (+) 1236 WP_087551350.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  CDG61_RS03340 (CDG61_03340) pilF 605469..606317 (+) 849 WP_087551349.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  CDG61_RS03345 (CDG61_03345) - 606308..607096 (+) 789 WP_087551348.1 helix-turn-helix domain-containing protein -
  CDG61_RS03350 (CDG61_03350) ispG 607122..608243 (+) 1122 WP_087551347.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  CDG61_RS03355 (CDG61_03355) hisS 608247..609542 (+) 1296 WP_087551346.1 histidine--tRNA ligase -
  CDG61_RS03360 (CDG61_03360) - 609572..610270 (+) 699 WP_087551345.1 YfgM family protein -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 31465.75 Da        Isoelectric Point: 9.8301

>NTDB_id=313860 CDG61_RS03340 WP_087551349.1 605469..606317(+) (pilF) [Acinetobacter sp. WCHAc010052]
MRYCVRKARSRGSQLGKSVLKIAFITTVCISTVLISGCQTPATIGQKDPEKAVKVRSQLAAEYIRSGDLDAAKRALDQAL
ESDPRDANANMMMGILLQQEGSKISMEKADSYFRRSISSDSKNAQARNNYGTYLYQVERYNEAVEQLSIAGATLGYDQRS
RALENLGRVYLKLGDQASAEKAFRQALQANRNSFVSMLELSEILYLNQQAESAGKLYEQFVRAVGQKNQGARALWIGIRI
ARANGDNLGMQVLVNQLRALFPDSQEYQSYLKLQHSTEAVWK

Nucleotide


Download         Length: 849 bp        

>NTDB_id=313860 CDG61_RS03340 WP_087551349.1 605469..606317(+) (pilF) [Acinetobacter sp. WCHAc010052]
ATGAGATATTGCGTTCGCAAGGCTAGATCAAGGGGAAGTCAATTGGGTAAGTCTGTTTTAAAAATTGCATTTATAACAAC
AGTCTGTATATCAACAGTGTTGATTTCAGGCTGTCAGACTCCCGCTACAATTGGACAGAAAGATCCTGAAAAAGCAGTCA
AAGTCAGATCTCAGCTGGCGGCTGAATACATTCGCAGTGGTGATCTTGATGCAGCGAAACGAGCACTGGATCAGGCACTT
GAGTCAGATCCACGTGATGCAAATGCCAATATGATGATGGGTATCCTGTTACAGCAGGAAGGCAGTAAAATCAGCATGGA
AAAAGCTGACAGCTATTTCCGCCGTTCCATCTCATCAGATTCTAAAAATGCGCAGGCGCGTAACAACTATGGTACGTACT
TATATCAGGTTGAGCGTTATAATGAGGCGGTTGAACAACTGAGTATTGCGGGAGCAACGCTTGGATATGATCAGCGTTCA
AGAGCACTTGAAAATCTTGGACGGGTATATCTGAAGCTGGGAGATCAGGCGAGTGCTGAAAAAGCATTCAGACAGGCACT
CCAGGCAAACCGTAATTCTTTTGTTTCAATGCTGGAATTATCAGAAATTCTTTATCTGAACCAGCAGGCTGAATCCGCAG
GGAAGCTGTATGAGCAGTTTGTCCGTGCAGTCGGTCAGAAAAATCAGGGAGCGCGTGCATTATGGATCGGTATCCGCATC
GCACGGGCCAATGGCGATAATCTGGGAATGCAGGTGCTGGTCAATCAGTTACGGGCTCTGTTTCCAGACAGCCAGGAATA
CCAAAGTTATTTGAAATTACAGCACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(59-94)

Tetratricopeptide repeat (TPR_12)

(124-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

73.764

93.262

0.688
  pilF Acinetobacter baylyi ADP1

68.775

89.716

0.617

Multiple sequence alignment