Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CDG60_RS15160 Genome accession   NZ_CP032134
Coordinates   2997475..2998227 (-) Length   250 a.a.
NCBI ID   WP_227542888.1    Uniprot ID   -
Organism   Acinetobacter chinensis strain WCHAc010005     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2992475..3003227
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG60_RS15140 (CDG60_15140) - 2993522..2994220 (-) 699 WP_087513062.1 tetratricopeptide repeat protein -
  CDG60_RS15145 (CDG60_15145) hisS 2994250..2995545 (-) 1296 WP_087513063.1 histidine--tRNA ligase -
  CDG60_RS15150 (CDG60_15150) ispG 2995549..2996670 (-) 1122 WP_087513064.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  CDG60_RS15155 (CDG60_15155) - 2996696..2997484 (-) 789 WP_087513065.1 DUF4115 domain-containing protein -
  CDG60_RS15160 (CDG60_15160) pilF 2997475..2998227 (-) 753 WP_227542888.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  CDG60_RS15165 (CDG60_15165) rlmN 2998298..2999533 (-) 1236 WP_087513067.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  CDG60_RS15170 (CDG60_15170) ndk 2999655..3000086 (-) 432 WP_087513068.1 nucleoside-diphosphate kinase -
  CDG60_RS15175 (CDG60_15175) iscX 3000208..3000408 (-) 201 WP_087513069.1 Fe-S cluster assembly protein IscX -
  CDG60_RS15180 (CDG60_15180) - 3000498..3001049 (-) 552 WP_087513070.1 phosphatase PAP2 family protein -
  CDG60_RS15185 (CDG60_15185) - 3001082..3002368 (-) 1287 WP_087513071.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 250 a.a.        Molecular weight: 27941.50 Da        Isoelectric Point: 9.0612

>NTDB_id=313832 CDG60_RS15160 WP_227542888.1 2997475..2998227(-) (pilF) [Acinetobacter chinensis strain WCHAc010005]
MLISGCQTPATIGQKDPEKAVKVRSQLAAEYIRSGDLDAAKRALDQALESDPRDANANMMMGILLQQEGSKISMEKADSY
FRRSISSDSKNAQARNNYGTYLYQVERYNEAVEQLSIAGATLGYDQRSRALENLGRVYLKLGDQASAEKAFRQALQANRN
SFVSMLELSEILYLNQQAESAGKLYEQFVRAVGQKNQGARALWIGIRIARANGDNLGMQVLVNQLRALFPDSQEYQSYLK
LQHSTEAVWK

Nucleotide


Download         Length: 753 bp        

>NTDB_id=313832 CDG60_RS15160 WP_227542888.1 2997475..2998227(-) (pilF) [Acinetobacter chinensis strain WCHAc010005]
ATGTTGATTTCAGGCTGTCAGACTCCCGCTACAATTGGACAGAAAGATCCTGAAAAAGCAGTCAAAGTCAGATCTCAGCT
GGCGGCTGAATACATTCGCAGTGGTGATCTTGATGCAGCGAAACGTGCACTGGATCAGGCACTTGAGTCAGATCCACGTG
ATGCGAATGCCAATATGATGATGGGTATCCTGTTACAGCAGGAAGGCAGCAAAATCAGCATGGAAAAAGCTGACAGCTAT
TTCCGCCGTTCCATCTCATCAGATTCCAAAAATGCGCAGGCGCGTAACAACTATGGTACGTACTTATATCAGGTTGAGCG
TTATAATGAGGCGGTTGAACAACTGAGTATTGCGGGAGCAACGCTTGGATATGATCAGCGTTCAAGAGCACTTGAAAATC
TTGGACGGGTATATCTGAAGCTGGGAGATCAGGCGAGTGCTGAAAAAGCATTCAGACAGGCACTCCAGGCAAACCGTAAT
TCTTTTGTTTCAATGCTGGAATTATCAGAAATTCTTTATCTGAACCAGCAGGCTGAATCCGCAGGGAAGCTGTATGAACA
GTTTGTCCGTGCAGTCGGTCAGAAAAATCAGGGAGCGCGTGCATTATGGATCGGTATCCGCATCGCACGGGCCAATGGCG
ATAATCTGGGAATGCAGGTGCTGGTCAATCAGTTACGGGCTCTGTTTCCAGACAGCCAGGAATACCAAAGTTATTTGAAA
TTACAGCACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(92-159)

Tetratricopeptide repeat (TPR_16)

(27-62)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

76

100

0.76
  pilF Acinetobacter baylyi ADP1

69.6

100

0.696

Multiple sequence alignment