Detailed information NTG0031343

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: ECD_RS13525
• Genome accession: NC_012971
• Coordinates: 2706527..2707519 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli BL21(DE3)
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 2701527..2712519

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ECD_RS13505 (ECD_02587)	otnK	2702302..2703564 (+)	1263	WP_000590371.1	3-oxo-tetronate kinase	-
ECD_RS13510 (ECD_02588)	ygbL	2703561..2704199 (+)	639	WP_001278994.1	aldolase	-
ECD_RS13515 (ECD_02589)	ygbM	2704204..2704980 (+)	777	WP_001136925.1	HPr family phosphocarrier protein	-
ECD_RS13520 (ECD_02590)	ygbN	2705069..2706433 (+)	1365	WP_000104459.1	GntP family transporter	-
ECD_RS13525 (ECD_02591)	rpoS	2706527..2707519 (-)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
ECD_RS13530 (ECD_02592)	nlpD	2707582..2708676 (-)	1095	WP_224692580.1	murein hydrolase activator NlpD	-
ECD_RS13535 (ECD_02593)	pcm	2708861..2709487 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
ECD_RS13540 (ECD_02594)	surE	2709481..2710242 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
ECD_RS13545 (ECD_02595)	truD	2710223..2711272 (-)	1050	WP_000568943.1	tRNA pseudouridine(13) synthase TruD	-
ECD_RS13550 (ECD_02596)	ispF	2711269..2711748 (-)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
ECD_RS13555 (ECD_02597)	ispD	2711748..2712458 (-)	711	WP_000246144.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=31343 ECD_RS13525 WP_000081550.1 2706527..2707519(-) (rpoS) [Escherichia coli BL21(DE3)]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=31343 ECD_RS13525 WP_000081550.1 2706527..2707519(-) (rpoS) [Escherichia coli BL21(DE3)]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGTGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715