Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   Ahae5227_RS02835 Genome accession   NZ_CP031988
Coordinates   594767..595567 (+) Length   266 a.a.
NCBI ID   WP_026056343.1    Uniprot ID   A0AAW4JFL0
Organism   Acinetobacter haemolyticus strain 5227     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 589767..600567
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Ahae5227_RS02810 (Ahae5227_02845) - 590677..591993 (+) 1317 WP_171402604.1 glycosyltransferase family 4 protein -
  Ahae5227_RS02815 (Ahae5227_02850) - 592017..592568 (+) 552 WP_004640826.1 phosphatase PAP2 family protein -
  Ahae5227_RS02820 (Ahae5227_02855) iscX 592652..592852 (+) 201 WP_004640824.1 Fe-S cluster assembly protein IscX -
  Ahae5227_RS02825 (Ahae5227_02860) ndk 592962..593393 (+) 432 WP_004640823.1 nucleoside-diphosphate kinase -
  Ahae5227_RS02830 (Ahae5227_02865) rlmN 593514..594749 (+) 1236 WP_005083389.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  Ahae5227_RS02835 (Ahae5227_02870) pilF 594767..595567 (+) 801 WP_026056343.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  Ahae5227_RS02840 (Ahae5227_02875) - 595558..596352 (+) 795 WP_004640817.1 helix-turn-helix domain-containing protein -
  Ahae5227_RS02845 (Ahae5227_02880) ispG 596374..597489 (+) 1116 WP_005083384.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  Ahae5227_RS02850 (Ahae5227_02885) hisS 597486..598778 (+) 1293 WP_008942386.1 histidine--tRNA ligase -
  Ahae5227_RS02855 (Ahae5227_02890) - 598803..599501 (+) 699 WP_004640812.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30220.21 Da        Isoelectric Point: 9.6050

>NTDB_id=313168 Ahae5227_RS02835 WP_026056343.1 594767..595567(+) (pilF) [Acinetobacter haemolyticus strain 5227]
MNTTRLKTTIAITVVLMAGGLTACQSTPSTKDPEKAVQVRTQLAAEYIRTRDLDSAKRSLDQALKLNSRDANANMMMGIL
LQQEGSKLNMEKADAYFKRAISSEPDNAQARNNYGTYLYQMQRYNEAIEQFTRAGATLGYDQRFQSLENLGRIYLLLGDV
TNAEKSFKQALQANRNATISMLELSEIFYLQQNNRDASQLYEQYVRSVGQRNQGARALWIGIRIARANADQLGMQVLVNQ
LRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=313168 Ahae5227_RS02835 WP_026056343.1 594767..595567(+) (pilF) [Acinetobacter haemolyticus strain 5227]
TTGAATACAACTCGACTAAAAACGACCATAGCGATAACAGTTGTACTTATGGCAGGTGGCTTGACAGCTTGCCAATCGAC
ACCATCTACGAAAGATCCAGAGAAAGCTGTTCAGGTTCGCACCCAATTGGCTGCGGAATATATTCGTACGCGTGATTTGG
ACTCTGCAAAACGTTCACTTGACCAAGCATTGAAACTTAATTCCCGTGATGCAAATGCCAATATGATGATGGGTATTTTG
CTGCAACAAGAGGGAAGTAAGCTCAATATGGAAAAAGCAGATGCTTATTTTAAGCGAGCGATTTCATCTGAGCCAGACAA
TGCTCAAGCGCGTAATAATTACGGTACATACTTATATCAGATGCAGCGTTATAATGAAGCAATTGAGCAGTTCACGCGTG
CTGGTGCAACCTTAGGTTATGATCAACGATTTCAATCACTGGAAAACTTAGGGCGAATCTATTTATTATTGGGCGATGTG
ACGAATGCTGAAAAATCATTCAAACAGGCGCTGCAAGCAAATCGAAATGCAACCATTTCAATGTTAGAGTTGTCGGAAAT
TTTCTATTTGCAACAAAACAATAGAGATGCATCCCAGTTATATGAACAATATGTTCGTAGCGTGGGACAGCGCAACCAAG
GTGCTAGAGCACTTTGGATCGGGATACGCATTGCTCGAGCGAATGCGGATCAGTTAGGAATGCAAGTGTTAGTCAACCAA
TTACGCGCTTTATTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_12)

(108-175)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

76.58

100

0.774
  pilF Acinetobacter baylyi ADP1

68.045

100

0.68

Multiple sequence alignment