Detailed information    

insolico Bioinformatically predicted

Overview

Name   recR   Type   Machinery gene
Locus tag   ECD_RS02215 Genome accession   NC_012971
Coordinates   463451..464056 (+) Length   201 a.a.
NCBI ID   WP_001195025.1    Uniprot ID   P0A7H7
Organism   Escherichia coli BL21(DE3)     
Function   promote later steps in plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 458451..469056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECD_RS02185 (ECD_00417) rsmS 459156..459317 (-) 162 WP_000051153.1 pleiotropic regulatory protein RsmS -
  ECD_RS02190 (ECD_00418) priC 459331..459858 (-) 528 WP_000844874.1 primosomal replication protein N'' -
  ECD_RS02195 (ECD_00419) ybaN 459928..460305 (+) 378 WP_001188905.1 DUF454 family protein -
  ECD_RS02200 (ECD_00420) apt 460458..461009 (+) 552 WP_000127356.1 adenine phosphoribosyltransferase -
  ECD_RS02205 (ECD_00421) dnaX 461138..463069 (+) 1932 WP_000122013.1 DNA polymerase III subunit gamma/tau -
  ECD_RS02210 (ECD_00422) ybaB 463122..463451 (+) 330 WP_000467098.1 YbaB/EbfC family nucleoid-associated protein -
  ECD_RS02215 (ECD_00423) recR 463451..464056 (+) 606 WP_001195025.1 recombination mediator RecR Machinery gene
  ECD_RS02220 (ECD_00424) htpG 464166..466040 (+) 1875 WP_000678201.1 molecular chaperone HtpG -
  ECD_RS02225 (ECD_00425) adk 466221..466865 (+) 645 WP_001220233.1 adenylate kinase -
  ECD_RS02230 (ECD_00426) hemH 467101..468063 (+) 963 WP_001250117.1 ferrochelatase -
  ECD_RS02235 (ECD_00427) aes 468060..469019 (-) 960 WP_000801849.1 acetyl esterase -

Sequence

Protein


Download         Length: 201 a.a.        Molecular weight: 21963.00 Da        Isoelectric Point: 4.9097

>NTDB_id=31312 ECD_RS02215 WP_001195025.1 463451..464056(+) (recR) [Escherichia coli BL21(DE3)]
MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLAQALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQEN
GQICVVESPADIYAIEQTGQFSGRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF

Nucleotide


Download         Length: 606 bp        

>NTDB_id=31312 ECD_RS02215 WP_001195025.1 463451..464056(+) (recR) [Escherichia coli BL21(DE3)]
ATGCAAACCAGCCCGCTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCG
TATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTCGGAAA
TCGGCCACTGCGCCGATTGCCGCACTTTCACCGAACAGGAAGTCTGTAACATCTGTTCGAATCCGCGTCGTCAGGAAAAC
GGTCAAATCTGCGTGGTGGAGAGTCCGGCGGACATCTACGCCATTGAGCAGACGGGGCAGTTTTCAGGTCGTTATTTTGT
GTTGATGGGGCATCTGTCACCGCTGGACGGCATCGGTCCGGATGATATCGGGCTTGATCGTCTGGAACAGCGTCTGGCAG
AGGAAAAAATCACTGAAGTGATCCTCGCCACCAATCCCACGGTTGAAGGTGAAGCTACCGCTAACTACATTGCCGAGCTT
TGCGCGCAATATGACGTGGAAGCCAGCCGAATCGCTCATGGCGTACCGGTTGGCGGCGAACTGGAAATGGTCGATGGCAC
CACGCTGTCACACTCCCTTGCCGGGCGTCATAAGATTCGTTTTTAA

Domains

Predicted by InterProScan.

RecR protein (RecR_ZnF)

(41-77)

Toprim domain (Toprim_4)

(81-172)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P0A7H7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recR Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.226

99.005

0.448
  recR Bacillus subtilis subsp. subtilis str. 168

44.724

99.005

0.443

Multiple sequence alignment