Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   D1345_RS19070 Genome accession   NZ_CP031968
Coordinates   4170659..4172365 (-) Length   568 a.a.
NCBI ID   WP_019100887.1    Uniprot ID   A0AAD0RVK9
Organism   Chromobacterium rhizoryzae strain JP2-74     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4165659..4177365
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1345_RS19045 (D1345_19045) yacG 4166874..4167062 (-) 189 WP_019100892.1 DNA gyrase inhibitor YacG -
  D1345_RS19050 (D1345_19050) zapD 4167100..4167858 (-) 759 WP_019100891.1 cell division protein ZapD -
  D1345_RS19055 (D1345_19055) coaE 4167897..4168508 (-) 612 WP_043591353.1 dephospho-CoA kinase -
  D1345_RS19060 (D1345_19060) pilD 4168498..4169367 (-) 870 WP_043591355.1 A24 family peptidase Machinery gene
  D1345_RS19065 (D1345_19065) pilC 4169364..4170587 (-) 1224 WP_107732856.1 type II secretion system F family protein Machinery gene
  D1345_RS19070 (D1345_19070) pilF 4170659..4172365 (-) 1707 WP_019100887.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D1345_RS19075 (D1345_19075) - 4172586..4173716 (-) 1131 WP_107732857.1 porin -
  D1345_RS19080 (D1345_19080) - 4173958..4175466 (+) 1509 WP_107732858.1 deoxyribodipyrimidine photo-lyase -
  D1345_RS19085 (D1345_19085) - 4175521..4175904 (-) 384 WP_240333834.1 helix-hairpin-helix domain-containing protein -
  D1345_RS19090 (D1345_19090) ccsA 4175936..4176757 (-) 822 WP_019103784.1 cytochrome c biogenesis protein CcsA -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62252.84 Da        Isoelectric Point: 5.4436

>NTDB_id=312968 D1345_RS19070 WP_019100887.1 4170659..4172365(-) (pilF) [Chromobacterium rhizoryzae strain JP2-74]
MEASAGISGLGRALVQNNMLLQQDADAILRQSQASHISFVEQLIQSKKMTAQDVAMFASKTFGYPLLDMSAIDLDQLPKG
VVDDELIASRRLLPLLKRGNKLYVGTSDPTQTSAYHAITFKTGMTVEIVVVEDDKLGKMLGKYSEDATAAIKALENEDFG
DLEFADPDAPTDTGPQPDVDDAPVVKFIHKVLLDAINGGASDIHFEPYEKFYRIRYRVDGVLKEISQPPLVLKEKIASRI
KVISKLDISEKRVPQDGRMKLVISKARAIDFRVSTLPTLYGEKIVMRILDSSAANLDIEQLGFEPEQKRLLLEAIGRPYG
MVLVTGPTGSGKTVSLYTCLNLLNKPDINIATAEDPVEINLPGINQVNVNERAGLTFASALKAFLRQDPDVIMVGEIRDF
ETADIAIKAAQTGHMVFSTLHTNNAPATLTRMLNMGVAPFNIASSVLLIMAQRLARRLCAHCKKPAEIPEEALLRAGFKQ
EELDGSWKPYAPVGCEECRNTGYRGRTGIYEVMPITDAMTRLIMQNGTAIDIADLARKEGMVDLRHAGLLKVKAGLTSLT
EIEAVTNE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=312968 D1345_RS19070 WP_019100887.1 4170659..4172365(-) (pilF) [Chromobacterium rhizoryzae strain JP2-74]
ATGGAAGCTTCGGCTGGGATTTCCGGCTTGGGTCGGGCCTTGGTGCAAAACAATATGCTGTTGCAGCAGGATGCGGACGC
CATTCTGCGCCAGTCTCAGGCGTCGCATATCAGCTTCGTCGAGCAGCTGATTCAGAGCAAGAAGATGACGGCGCAAGATG
TTGCGATGTTCGCGTCCAAGACCTTTGGTTACCCCTTGTTGGACATGTCGGCCATTGATCTTGATCAGTTGCCCAAGGGC
GTGGTTGATGACGAGTTGATCGCCAGCCGGCGCTTGCTGCCCCTGCTCAAGCGCGGCAACAAGCTGTATGTAGGCACCTC
CGATCCCACCCAGACCTCGGCCTATCACGCCATCACCTTCAAGACCGGTATGACGGTGGAAATCGTGGTGGTGGAGGATG
ATAAGCTGGGCAAGATGCTTGGCAAGTACAGCGAGGACGCCACCGCCGCAATCAAGGCTCTGGAGAACGAGGATTTTGGC
GATCTTGAATTCGCCGATCCGGATGCGCCGACGGATACCGGTCCACAGCCAGATGTCGACGATGCGCCGGTGGTCAAGTT
CATTCATAAGGTGTTGTTGGACGCTATCAATGGAGGCGCGTCCGATATTCATTTCGAGCCCTATGAGAAGTTTTACCGCA
TTCGCTACCGGGTAGACGGGGTGTTGAAGGAGATTTCACAGCCGCCTCTGGTATTGAAGGAAAAGATCGCCTCTCGGATC
AAGGTGATTTCCAAGTTGGATATTTCGGAGAAGCGGGTGCCGCAGGACGGTCGGATGAAGCTGGTCATTTCCAAGGCGCG
GGCGATCGACTTCCGGGTCAGCACCTTGCCCACCTTGTACGGCGAGAAGATCGTGATGCGTATTCTGGACTCGTCGGCGG
CGAATCTGGATATCGAGCAGCTGGGCTTCGAGCCGGAGCAGAAACGGTTGTTGCTGGAGGCTATCGGACGCCCCTACGGG
ATGGTGTTGGTTACCGGTCCCACCGGCAGTGGTAAAACGGTGTCGCTGTATACCTGTCTTAATTTGTTGAACAAGCCGGA
CATCAATATCGCCACGGCGGAAGACCCTGTGGAAATCAACTTGCCGGGCATCAATCAGGTCAACGTCAACGAGCGAGCCG
GCTTGACCTTCGCTTCGGCCTTGAAGGCCTTCCTGCGTCAGGATCCGGACGTGATCATGGTGGGTGAGATCCGGGATTTC
GAAACGGCGGACATCGCGATCAAGGCGGCTCAGACTGGCCATATGGTGTTTTCCACTCTACACACCAATAACGCGCCGGC
GACTTTGACCCGGATGCTGAATATGGGGGTGGCGCCGTTCAATATTGCCAGCTCGGTGCTGCTGATCATGGCGCAGCGTT
TGGCGCGTCGTCTGTGCGCGCATTGCAAGAAACCTGCGGAGATTCCGGAGGAGGCATTGCTGCGGGCGGGATTCAAGCAA
GAGGAGTTGGACGGAAGCTGGAAGCCCTATGCGCCGGTAGGGTGCGAGGAGTGCCGCAACACTGGCTACCGCGGCCGTAC
CGGCATTTACGAAGTGATGCCGATTACCGACGCGATGACGCGTTTGATCATGCAGAATGGCACCGCCATCGATATCGCCG
ACTTGGCGCGCAAGGAGGGCATGGTGGACTTGCGCCACGCCGGTTTGCTGAAGGTGAAGGCGGGCTTGACCTCGTTGACC
GAGATCGAAGCGGTGACTAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

56.637

99.472

0.563
  pilB Acinetobacter baumannii D1279779

54.059

100

0.551
  pilB Acinetobacter baylyi ADP1

53.996

99.12

0.535
  pilB Legionella pneumophila strain ERS1305867

51.839

100

0.521
  pilB Vibrio cholerae strain A1552

49.46

97.887

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

47.331

98.944

0.468
  pilB Vibrio campbellii strain DS40M4

45.826

99.12

0.454
  pilF Thermus thermophilus HB27

38.121

99.296

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.722

98.944

0.373

Multiple sequence alignment