Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   D1367_RS21310 Genome accession   NZ_CP031941
Coordinates   4785260..4786786 (-) Length   508 a.a.
NCBI ID   WP_118168136.1    Uniprot ID   -
Organism   Nostoc sphaeroides strain Kutzing En     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4780260..4791786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1367_RS21275 - 4780458..4781312 (-) 855 WP_118168130.1 Uma2 family endonuclease -
  D1367_RS21280 - 4781754..4782221 (+) 468 WP_118168131.1 hypothetical protein -
  D1367_RS21285 - 4783070..4783198 (-) 129 WP_118168132.1 HNH endonuclease -
  D1367_RS21290 - 4783191..4783430 (-) 240 WP_118168133.1 hypothetical protein -
  D1367_RS21300 - 4783682..4783960 (-) 279 WP_118168134.1 hypothetical protein -
  D1367_RS21305 - 4784286..4785035 (-) 750 WP_118168135.1 Uma2 family endonuclease -
  D1367_RS21310 radA 4785260..4786786 (-) 1527 WP_118168136.1 DNA repair protein RadA Machinery gene
  D1367_RS21315 rpaB 4786957..4787685 (+) 729 WP_118168137.1 response regulator transcription factor RpaB -
  D1367_RS21325 - 4787782..4788420 (+) 639 WP_118168138.1 cofactor assembly of complex C subunit B -
  D1367_RS21330 - 4789210..4789728 (+) 519 WP_118168139.1 DUF456 domain-containing protein -
  D1367_RS21335 - 4790534..4791193 (+) 660 WP_118168140.1 PEP-CTERM sorting domain-containing protein -

Sequence

Protein


Download         Length: 508 a.a.        Molecular weight: 54593.48 Da        Isoelectric Point: 4.8256

>NTDB_id=312846 D1367_RS21310 WP_118168136.1 4785260..4786786(-) (radA) [Nostoc sphaeroides strain Kutzing En]
MAKPKTFFVCNECGAESPQWFGKCPGCGTYNSLEEQITIQSSVDVPSRGGVSGWQSTQSNGKSHAKPAKARASLTFDQIT
DRQIARWESGYEELDRVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSNQLAQKYRILYVTGEESGQQVKLRASRLGVSKTL
SVLNEEDVKVVVDPTLPIDPDSIGADLYVLPETDLEEILREIDSLKPNVAVIDSIQTVFFPALTSAPGSVAQVRECTAAL
MKVAKHEDVTMLIVGHVTKEGAIAGPKVLEHLVDTVLYFEGDRFASHRLLRTVKNRFGATHEIGIFEMVAEGLREVSNPS
ELFLGNRDDPAPGTAIVVACEGTRPIVVELQALVSPTSYPSPRRAGTGVDYNRLVQILAVLEKRVGIPMSKLDSYVASAG
GLNVEEPAVDLGIAIAIVASFRDRIVDPGTVLIGEVGLGGQVRSVSQMELRLKEAAKLGFKRAIVPKGTKFPDLNIEILP
VSKVIDAIIAAIPHQELTADDLEPDEDE

Nucleotide


Download         Length: 1527 bp        

>NTDB_id=312846 D1367_RS21310 WP_118168136.1 4785260..4786786(-) (radA) [Nostoc sphaeroides strain Kutzing En]
ATGGCAAAGCCAAAAACCTTTTTTGTGTGTAATGAATGTGGAGCAGAATCGCCCCAGTGGTTTGGTAAATGTCCAGGTTG
CGGCACTTACAACTCTCTAGAAGAACAGATCACTATTCAATCCTCAGTAGATGTACCCAGTCGAGGGGGAGTGAGTGGTT
GGCAATCAACTCAAAGTAATGGCAAATCTCACGCTAAACCAGCTAAAGCGCGAGCCTCCTTAACATTTGACCAAATTACC
GATCGCCAAATTGCCCGATGGGAGTCTGGTTATGAAGAATTGGATCGGGTGCTTGGGGGTGGGGTTGTCCCTGGCTCGAT
GGTACTGATTGGCGGCGATCCGGGTATTGGTAAATCGACTTTATTACTGCAAGTATCTAATCAATTAGCACAGAAATACC
GCATCCTTTACGTAACTGGTGAAGAATCGGGACAGCAGGTAAAATTACGAGCTTCCCGTTTAGGAGTATCAAAAACCCTA
AGTGTGCTTAATGAGGAGGATGTTAAGGTAGTAGTAGATCCTACACTTCCTATAGACCCTGACAGTATAGGTGCAGATTT
ATATGTACTGCCAGAAACAGATTTAGAAGAGATTTTACGGGAAATAGATTCTCTCAAGCCAAACGTGGCGGTGATTGATA
GTATCCAAACAGTATTTTTTCCGGCGTTGACTTCTGCACCCGGTTCAGTAGCTCAGGTACGGGAATGTACGGCAGCATTG
ATGAAGGTGGCGAAGCACGAAGATGTCACTATGCTGATTGTCGGACACGTCACCAAAGAAGGAGCGATCGCCGGGCCAAA
AGTTTTAGAACATTTAGTTGATACAGTGTTGTATTTTGAAGGCGATCGCTTTGCCTCCCATCGGTTATTACGGACAGTAA
AAAACCGTTTTGGTGCAACTCACGAAATCGGCATTTTTGAAATGGTGGCTGAGGGACTACGGGAAGTCTCCAATCCCTCA
GAGCTATTTTTAGGCAACCGTGATGATCCCGCACCCGGTACGGCAATTGTGGTTGCTTGCGAAGGTACTCGCCCCATCGT
TGTAGAGTTGCAAGCTTTGGTGAGTCCCACCAGCTACCCCTCACCCCGGCGGGCTGGAACTGGCGTAGACTACAACCGCC
TAGTGCAAATTCTCGCCGTCTTAGAAAAACGGGTGGGAATTCCCATGTCCAAGTTAGATTCTTACGTTGCTTCTGCGGGT
GGGTTGAATGTAGAAGAACCGGCGGTAGATTTGGGAATTGCGATCGCGATCGTAGCCAGTTTCCGCGATCGCATCGTCGA
TCCCGGTACAGTATTAATCGGTGAAGTTGGATTAGGCGGACAAGTGCGATCGGTTTCCCAAATGGAACTGCGGTTAAAAG
AAGCTGCTAAGTTGGGATTTAAAAGAGCGATCGTGCCAAAAGGAACAAAATTCCCCGACTTGAATATTGAAATATTACCC
GTCTCTAAAGTTATAGATGCAATTATCGCCGCCATCCCGCATCAAGAATTGACAGCCGACGATTTAGAACCCGATGAAGA
TGAGTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(402-467)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

KaiC (ATPase)

(89-296)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.304

97.047

0.488
  radA Streptococcus mitis SK321

46.436

96.654

0.449
  radA Streptococcus pneumoniae Rx1

47.974

92.323

0.443
  radA Streptococcus pneumoniae D39

47.974

92.323

0.443
  radA Streptococcus pneumoniae R6

47.974

92.323

0.443
  radA Streptococcus pneumoniae TIGR4

47.974

92.323

0.443
  radA Streptococcus mitis NCTC 12261

47.974

92.323

0.443

Multiple sequence alignment